A5054986036- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Hippo pathway signaling and YAP/TAZ
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Cancer Research and Treatments
- Biofuel production and bioconversion
- Microtubule and mitosis dynamics
- Enzyme Production and Characterization
- Analytical Chemistry and Chromatography
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Autophagy in Disease and Therapy
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Synthesis and Analysis
- Cancer Mechanisms and Therapy
- Pharmacological Effects of Natural Compounds
- Phagocytosis and Immune Regulation
- Polyamine Metabolism and Applications
- Ubiquitin and proteasome pathways
- Microbial Metabolism and Applications
- Pharmacogenetics and Drug Metabolism
- Pancreatic function and diabetes
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolites in Food Biotechnology
Zhejiang University of Technology
2016-2025
Chemical Synthesis Lab
2025
Biocon (India)
2025
Guangxi Zhuang Autonomous Region Brain Hospital
2025
First People's Hospital of Chongqing
2024
South China Agricultural University
2020-2023
Hangzhou Medical College
2022
Zhejiang Provincial People's Hospital
2022
Science and Technology Department of Sichuan Province
2021
Hunan Provincial Maternal and Child Health Hospital
2010-2021
Neurofibromatosis 2 (NF2) is a tumor suppressor, although the molecular mechanism accounting for this effect remains unknown. Here, we show that merlin exerts its activity by inhibiting phosphatidylinositol 3-kinase (PI3-kinase), through binding to PIKE-L. Wild-type merlin, but not patient-derived mutant (L64P), binds PIKE-L and inhibits PI3-kinase activity. This suppression of results from disrupting PI3-kinase. In addition, as well schwannoma cell growth abrogated single point mutation...
L-methionine has attracted a great deal of attention for its nutritional, pharmaceutical, and clinical applications. In this study, Escherichia coli W3110 was engineered via deletion negative transcriptional regulator MetJ over-expression homoserine O-succinyltransferase MetA together with efflux transporter YjeH, resulting in overproduction which is up to 413.16 mg/L. The partial inactivation the import system MetD disruption metI made E. ΔmetJ ΔmetI/pTrcA*H more tolerant high L-ethionine...
The development of theranostic systems capable diagnosis, therapy, and target specificity is considerably significant for accomplishing personalized medicine. Here, a multifunctional rattle‐type nanoparticle (MRTN) as an effective biological bimodal imaging tumor‐targeting delivery system fabricated, enhanced loading ability hydrophobic anticancer drug (paclitaxel) also realized. rattle structure with Fe 3 O 4 the inner core mesoporous silica shell obtained by one‐step templates removal...
A strategy is presented to control the disassembly of nanogels using near-infrared light. Novel diselenide-cross-linked with a newly synthesized diselenide cross-linker are prepared. Indocyanine green incorporated into trigger cleavage bond through generating reactive oxygen species. The appropriate illumination allows for on-demand degradation and controlled drug release. As service our authors readers, this journal provides supporting information supplied by authors. Such materials peer...
Thionins are important antibacterial peptides in plants. However, the roles of plant thionins, especially defensin-dissimilar alleviating heavy-metal toxicity and accumulation remain unclear. Here, cadmium (Cd)-related functions mechanisms rice thionin OsThi9 were investigated. was significantly upregulated response to Cd exposure. localized cell wall shown bind Cd; these characters help increase tolerance. In Cd-exposed plants, overexpression increased binding, decreasing upward...
Protein engineering is a powerful tool for improving the properties of enzymes. However, large changes in enzyme are still challenging traditional evolution strategies because they usually require multiple amino acid substitutions. In this study, feasible approach by combination fragment swapping and semi-rational design was developed nitrilase. A chimera BaNIT harboring 12 substitutions obtained using nitrilase from Arabis alpine (AaNIT) Brassica rapa (BrNIT) as parent enzymes, which...
Abstract We assessed mpox virus prevalence in blood, pharyngeal, and rectal specimens among persons without characteristic rash presenting for JYNNEOS vaccine. Our data indicate that the utility of risk-based screening skin lesions or via pharyngeal swabs, and/or blood is likely limited.
Monodisperse poly(vinylcaprolactam) (PVCL)-based capsules are prepared by precipitation polymerization of vinylcaprolactam (VCL) onto dimethyldiethoxysilane (DMDES) emulsion droplets and removal the DMDES templates ethanol. Polymer chains in shells can be cross-linked during disulfide-containing cross-linker N,N′-bis(acryloyl) cystamine, which endows with an excellent redox-labile property. Versatility this technique to prepare diverse components is demonstrated copolymerization methacrylic...
Extracellular and intracellular biosynthesis of silver nanoparticles (AgNPs) by Euglena gracilis (EG) strain intermedia (EI) are reported in this study. The obtained showed an absorption peak approximates 420 nm the UV-visible spectrum, corresponding to plasmon resonance AgNPs. According result inductively coupled plasma-atomic emission spectrometer, intakes ions EI EG roughly equal. transmission electron microscope (TEM) analysis successful vivo vitro synthesised AgNPs indicated sizes,...
Nitrilases are a class of enzymes that hydrolyze nitriles to carboxylic acids and ammonia. However, as research has progressed, the hydration activities convert amides also found in nitrilases from different sources, which result difficulties for high-purity production meanwhile endow enzyme with potential valuable biosynthesis. In this study, distance between residues Cys Glu catalytic triad (DC-E), well cyano group nitrile substrates (DCN-E) were determined coaffect reaction pathway. A...
O-Succinyl-L-homoserine (OSH) is an important C4 platform compound with broad applications. Its green and efficient production receiving increasing attention. Herein, the OSH producing chassic cell was constructed by deleting transcriptional negative regulation factor, blocking consumption pathway, inhibiting competitive bypass pathways. The precursor synthesis pathways of aspartic acid homoserine were further strengthened, pentose phosphate pathway glycolysis modified to enhance NADPH...
Abstract The NF2 tumor suppressor gene encodes an intracellular membrane-associated protein, called merlin, which belongs to the band 4.1 family of cytoskeleton-associated proteins that link cell surface glycoproteins actin cytoskeleton. Merlin suppresses phosphatidylinositol 3-kinase (PI3K)/Akt signaling by directly binding and inhibiting stimulatory activity PIKE-L on PI3K. Akt feeds back phosphorylates merlin provokes its polyubiquitination degradation. Here, we show phosphorylation...
The production of fatty acid-derived chemicals has received a great deal attention in recent years. In yeast cells, the main storage forms acids are TAGs. However, conversion TAGs into acid derivatives suffers from practical standpoint. Herein, more direct strategy was applied to accumulate cellular acyl-CoAs Saccharomyces cerevisiae, which activated and used as important precursors for various converting enzymes. dga1 gene deleted block dependent pathway synthesis significant decrease lipid...