- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Agricultural Productivity and Crop Improvement
- Plant Taxonomy and Phylogenetics
- Plant tissue culture and regeneration
- Agriculture and Biological Studies
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Bioenergy crop production and management
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Rice Cultivation and Yield Improvement
- Plant Pathogens and Resistance
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Crop Yield and Soil Fertility
- Silicon Effects in Agriculture
- Phytase and its Applications
- Horticultural and Viticultural Research
- Agriculture, Plant Science, Crop Management
- Food composition and properties
- Mycotoxins in Agriculture and Food
- Botanical Research and Chemistry
Vavilov Institute of General Genetics
2015-2024
Engelhardt Institute of Molecular Biology
2013-2023
Siberian Branch of the Russian Academy of Sciences
2021-2023
Institute of Cytology and Genetics
2008-2023
Russian Academy of Sciences
1996-2015
Agriculture and Agri-Food Canada
2010
N.I. Vavilov Research Institute of Plant Industry
1986-2010
Institut National de la Recherche Agronomique
2007
Kansas State University
1996-1999
Abstract Key message Here, we provide an updated set of guidelines for naming genes in wheat that has been endorsed by the research community. The last decade seen a proliferation genomic resources wheat, including reference- and pan-genome assemblies with gene annotations, which new opportunities to detect, characterise, describe influence traits interest. expansion genetic information supported growth community catalysed strong interest control agronomically important traits, such as...
Summary Bread wheat ( Triticum aestivum ) is one of the most important crops worldwide. However, because its large, hexaploid, highly repetitive genome it a challenge to develop efficient means for molecular analysis and genetic improvement in wheat. To better understand composition evolution hexaploid homoeologous genomes evaluate potential BAC‐end sequences (BES) marker development, we have followed chromosome‐specific strategy generated 11 Mb random BES from chromosome 3B, largest bread...
Abstract Bread wheat (Triticum aestivum) inflorescences, or spikes, are characteristically unbranched and normally bear one spikelet per rachis node. Wheat mutants on which supernumerary spikelets (SSs) develop particularly useful resources for work towards understanding the genetic mechanisms underlying inflorescence architecture and, ultimately, yield components. Here, we report characterization of genetically unrelated leading to identification FRIZZY PANICLE (FZP) gene, encoding a member...
Four hundred and sixty polyploid wheat accessions 39 triticale forms from 37 countries of Europe, Asia, USA were scored by C-banding for the presence translocations. Chromosomal rearrangements detected in 70 208 tetraploid wheat, 69 252 hexaploid 3 forms. Altogether, 58 types major chromosomal identified studied material; they are discussed relative to 11 additional translocation described other authors. Six chromosome modifications unknown origin also observed. Among all aberrations single...
Introgressions from crop wild relatives (CWRs) have been used to introduce beneficial traits into cultivated plants. traditionally detected using cytological methods. Recently, single nucleotide polymorphism (SNP)-based methods proposed detect introgressions in crosses for which both parents are known. However, unknown material, no method was available and predict the putative donor species. Here, we present a We demonstrate utility of this 10 publicly wheat genome sequences identify nine...
Genome differentiation in 12 diploid Aegilops species was analyzed using situ hybridization with the highly repetitive DNA sequences pSc119 and pAs1 C-banding. Chromosomes of all these hybridized probe; however, level labeling patterns differed among genomes. Only four (Ae. squarrosa, Ae. comosa, heldreichii, uniaristata) showed distinct pAs1. The were species-specific chromosome-specific. Differences (ISH) patterns, also observed by C-banding, exist between karyotypes comosa suggesting that...
The distribution of the 5S and 18S-5.8S-26S (18S-26S) ribosomal RNA (rRNA) gene families on chromosomes all diploid Aegilops species was studied by in situ hybridization with pTa71 (18S-26S rDNA) pTa794 (5S DNA clones. One major 18S-26S rDNA locus found nucleolus organizer region (NOR) each tauschii uniaristata two loci were detected remaining species. In addition to NORs, from one nine minor observed; their numbers chromosomal locations species-specific. Some polymorphic, whereas others...
The evolution of 2 tandemly repeated sequences Spelt1 and Spelt52 was studied in Triticum species representing evolutionary lineages wheat Aegilops sect. Sitopsis, putative donors their B/G genomes. Using fluorescence situ hybridization we observed considerable polymorphisms the patterns repeats between within species. Between 28 subtelomeric sites probe were detected Ae. speltoidies, depending on accession. From 8 to 12 Timopheevi group (GAt genome), whereas number signals emmer/aestivum...
Karyotypes of 3 diploid wheat species containing different variants the A-genome, <i>Triticum boeoticum </i>(A<sup>b</sup>),<i> T. monococcum </i>(A<sup>b</sup>), and <i>T. urartu </i>(A<sup>u</sup>), were examined using C-banding FISH with DNA probes representing 5S 45S rDNA families, microsatellite sequences GAA<sub>n</sub> GTT<sub>n</sub>, already known satellite pSc119.2, Spelt52,...
Five diploid Aegilops species of the Sitopsis section: Ae. speltoides, longissima, sharonensis, searsii, and bicornis, two tetraploid peregrina (=Ae. variabilis) kotschyi (Aegilops section) hexaploid vavilovii (Vertebrata carry S-genomes. The B- G-genomes polyploid wheat are also derivatives S-genome. Evolution S-genome was studied using Giemsa C-banding fluorescence in situ hybridization (FISH) with DNA probes representing 5S (pTa794) 18S-5.8S-26S (pTa71) rDNAs as well nine tandem repeats:...
The genus Trifolium (Leguminosae), which includes several important forage legumes of temperate and subtropical regions, is characterized by small chromosomes uniform size. Cytogenetic mapping the two multigene families coding for 18S-5.8S-26S rRNA 5S in white clover ( T. repens) seven closely related species/subspecies was carried out using fluorescence situ hybridization (FISH). 18S-26S rDNA, generally confined to nucleolus organizer regions (NORs), consistently located proximally on...
Deschampsia antarctica Desv. (Poaceae) (2n = 26) is one of the two vascular plants adapted to harshest environment Antarctic. Although species a valuable model for study environmental stress tolerance in plants, its karyotype still poorly investigated. We firstly conducted comprehensive molecular cytogenetic analysis D. collected on four islands Maritime karyotypes were studied by Giemsa C- and DAPI/C-banding, Ag-NOR staining, multicolour fluorescence situ hybridization with repeated DNA...
We present a comprehensive survey of cytogenetic and genomic diversity the GGAtAt genepool wheat, thereby unlocking these plant genetic resources for wheat improvement. Wheat yields are stagnating around world new sources genes resistance or tolerances to abiotic traits required. In this context, tetraploid wild relatives among key candidates Despite its potential huge value breeding, is largely neglected. Understanding population structure, native distribution range, intraspecific variation...
This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) the supposed ancestors of hexaploid intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive probes proved suitable identification individual chromosomes JJ, StSt PP...
B chromosomes (Bs) are dispensable components of the genome exhibiting non-Mendelian inheritance. Chromosome counts and flow cytometric analysis grass species Aegilops speltoides revealed a tissue-type specific distribution roughly 570 Mbp large chromosomes. To address question whether organelle-to-nucleus DNA transfer is mechanism that drives evolution Bs, in situ hybridization was performed with labelled organellar DNA. The observed B-specific accumulation chloroplast- mitochondria-derived...
Markers linked to agronomic traits are of the prerequisite for molecular breeding. Genotyping-by-sequencing (GBS) data enables detect small polymorphisms including single nucleotide (SNPs) and short insertions or deletions (InDels) that can be used, instance, marker-assisted selection, population genetics, genome-wide association studies. Here, we aim at detecting large chromosomal modifications in barley wheat based on GBS data. These could duplications, deletions, substitutions...
Emmer wheat, Triticum dicoccon schrank (syn. T. dicoccum (schrank) schÜbl.), is one of the earliest domesticated crops, harboring a wide range genetic diversity and agronomically valuable traits. The crop, however, currently largely neglected. We provide wealth karyotypic information from comprehensive collection emmer wheat related taxa. In addition to C-banding polymorphisms, we identified 43 variants chromosomal rearrangements in dicoccon; among them 26 (60.4%) were novel. T7A:5B...
Species belonging to the genus Aegilops L. are an important source of genetic material for expanding variability wheat. Ae. triuncialis is allotetraploid in this which was originated from hybridization umbellulata and markgrafii (Greuter) Hammer. Although karyotype thoroughly examined by conventional chromosome staining Giemsa C-banding, it still poorly characterized using FISH markers. The objective study test fluorescence situ (FISH) patterns (2n = 4x 28, C(t)C(t)U(t)U(t)) chromosomes...