A5006858016- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Lignin and Wood Chemistry
- Plant Reproductive Biology
- Biofuel production and bioconversion
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Biochemical and biochemical processes
- Enzyme-mediated dye degradation
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Bioenergy crop production and management
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- African Botany and Ecology Studies
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant Taxonomy and Phylogenetics
- Yeasts and Rust Fungi Studies
Biopolymères Interactions Assemblages
2014-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
1998-2025
Laboratoire D'étude des Résidus et Contaminants Dans les Aliments
2024
Institut Jean-Pierre Bourgin
2014-2023
AgroParisTech
2013-2023
Institute for Structural Research
2023
Centre National de la Recherche Scientifique
2014-2020
Université Paris-Saclay
2015-2020
University of British Columbia
2018-2020
Institut National de la Recherche Agronomique
2002-2015
Plants are built of various specialized cell types that differ in their wall composition and structure. The walls certain tissues (xylem, sclerenchyma) characterized by the presence heterogenous polymer lignin plays an essential role physiology. This phenolic is composed different monomeric subunits - monolignols linked together several covalent bonds. Numerous studies have shown monolignol biosynthesis polymerization to form tightly controlled tissues. However our understanding genetic...
Over the past several years, Brachypodium distachyon (Brachypodium) has emerged as a tractable model system to study biological questions relevant grasses. To place its relevance in larger context of plant biology, we outline here expanding adoption grass and compare this early history another model, Arabidopsis thaliana. In context, followed an accelerated path which development genomic resources, most notably whole genome sequence, occurred concurrently with generation other experimental...
Abstract Secondary growth of the vasculature results in thickening plant structures and continuously produces xylem tissue, major biological carbon sink. Little is known about developmental control this quantitative trait, which displays two distinct phases Arabidopsis thaliana hypocotyls. The later phase accelerated expansion resembles secondary trees triggered upon flowering by an unknown, shoot-derived signal. We found that flowering-dependent hypocotyl a general feature herbaceous plants...
Plants have convergently evolved to use monolignol ferulate conjugates produce lignins containing chemically labile backbone esters.
Abstract Bread wheat (Triticum aestivum) inflorescences, or spikes, are characteristically unbranched and normally bear one spikelet per rachis node. Wheat mutants on which supernumerary spikelets (SSs) develop particularly useful resources for work towards understanding the genetic mechanisms underlying inflorescence architecture and, ultimately, yield components. Here, we report characterization of genetically unrelated leading to identification FRIZZY PANICLE (FZP) gene, encoding a member...
Grass lignins contain substantial amounts of p-coumarate (pCA) that acylate the side-chains phenylpropanoid polymer backbone. An acyltransferase, named p-coumaroyl-CoA:monolignol transferase (OsPMT), could monolignols with pCA in vitro was recently identified from rice. In planta, such monolignol-pCA conjugates become incorporated into lignin via oxidative radical coupling, thereby generating observed appendages; however p-coumarates also arabinoxylans grasses. To test authenticity PMT as a...
Brachypodium distachyon (Brachypodium) has been proposed as a model for grasses, but there is limited knowledge regarding its lignins and no data on lignin-related mutants. The cinnamyl alcohol dehydrogenase (CAD) genes involved in lignification are promising targets to improve the cellulose-to-ethanol conversion process. Down-regulation of CAD often induces reddish coloration lignified tissues. Based this observation, we screened chemically induced population mutants (Bd21-3 background) red...
Grass lignins can contain up to 10% 15% by weight of p-coumaric esters. This acylation is performed on monolignols under the catalysis p-coumaroyl-coenzyme A monolignol transferase (PMT). To study impact p-coumaroylation lignification, we first introduced Brachypodium distachyon Bradi2g36910 (BdPMT1) gene into Arabidopsis (Arabidopsis thaliana) control constitutive maize (Zea mays) ubiquitin promoter. The resulting was far lower than that from mature grass stems and had no stem lignin...
Lignin is an important phenolic biopolymer that provides strength and rigidity to the secondary cell walls of tracheary elements, sclereids, fibers in vascular plants. precursors, called monolignols, are synthesized exported wall where they polymerized into lignin by oxidative enzymes such as laccases peroxidases. In Arabidopsis thaliana, a peroxidase (PRX64) laccase (LAC4) shown localize differently within domains interfascicular fibers: PRX64 localizes middle lamella whereas LAC4...
The new model plant for temperate grasses, Brachypodium distachyon offers great potential as a tool functional genomics. We have established sodium azide-induced mutant collection and TILLING platform, called “BRACHYTIL”, the inbred line Bd21-3. consists of DNA isolated from 5530 different families. Phenotypes were reported organized in phenotypic tree that is freely available online. tilling platform was validated by isolation mutants seven genes belonging to multigene families lignin...
Abstract The oxidation of monolignols is a required step for lignin polymerization and deposition in cell walls. In dicots, both peroxidases laccases are known to participate this process. Here, we provide evidence that also involved the lignification Brachypodium distachyon, model plant temperate grasses. Transcript quantification data as well situ immunolocalization experiments demonstrated at least two (LACCASE5 LACCASE6) present lignifying tissues. A mutant with misspliced LACCASE5...
Significance Bioethanol produced from waste biomass crops has the potential to provide a sustainable alternative petroleum-based transportation fuel that does not compete with human food supply. The main obstacle this approach is resistance of digestion. Thus, expensive energetic pretreatment and high enzyme inputs are needed increase In study, we screened population randomly mutated plants for digestibility aim identifying novel factors impact on trait. We found number mutants no...
Abstract Grasses have varying inflorescence shapes; however, little is known about the genetic mechanisms specifying such shapes among tribes. Here, we identify grass-specific TCP transcription factor COMPOSITUM 1 (COM1) expressing in meristematic boundaries of different grasses. COM1 specifies branch-inhibition barley (Triticeae) versus branch-formation non-Triticeae Analyses cell size, walls and transcripts reveal regulates growth, thereby affecting wall properties signaling specifically...
Daylength sensing in many plants is critical for coordinating the timing of flowering with appropriate season. Temperate climate-adapted grasses such as Brachypodium distachyon flower during spring when days are becoming longer. The photoreceptor PHYTOCHROME C essential long-day (LD) B . PHYC required LD activation a suite genes photoperiod pathway including PHOTOPERIOD1 ( PPD1 ) that, turn, result FLOWERING LOCUS T FT1 )/ FLORIGEN , which causes flowering. Thus, phyC mutants extremely...
Cereal crop by-products are a promising source of renewable raw material for the production biofuel from lignocellulose. However, their enzymatic conversion to fermentable sugars is detrimentally affected by lignins. Here characterization Brachypodium Bd5139 mutant provided with single nucleotide mutation in caffeic acid O-methyltransferase BdCOMT6 gene reported. This BdCOMT6-deficient displayed moderately altered lignification mature stems. The lignin-related was also found be expressed...