A5017984677- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Water Relations and Carbon Dynamics
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Genetically Modified Organisms Research
- Plant Taxonomy and Phylogenetics
- Greenhouse Technology and Climate Control
- Lignin and Wood Chemistry
- Enzyme-mediated dye degradation
- Plant Pathogens and Fungal Diseases
- Forest ecology and management
- Tree Root and Stability Studies
- Plant Genetic and Mutation Studies
- Animal Genetics and Reproduction
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Seed Germination and Physiology
- Bioenergy crop production and management
- Irrigation Practices and Water Management
- Biofuel production and bioconversion
- Mycotoxins in Agriculture and Food
- Plant responses to water stress
Institut Jean-Pierre Bourgin
2012-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2023
AgroParisTech
2012-2023
Université Paris-Saclay
2017-2023
Centre National de la Recherche Scientifique
2015-2019
Département Biologie et Amélioration des Plantes
2008
Laboratoire de Biologie et Modélisation de la Cellule
2008
Institut National de la Recherche Agronomique
2006
Brachypodium distachyon (Brachypodium) has been proposed as a model for grasses, but there is limited knowledge regarding its lignins and no data on lignin-related mutants. The cinnamyl alcohol dehydrogenase (CAD) genes involved in lignification are promising targets to improve the cellulose-to-ethanol conversion process. Down-regulation of CAD often induces reddish coloration lignified tissues. Based this observation, we screened chemically induced population mutants (Bd21-3 background) red...
To test whether natural variation in Arabidopsis could be used to dissect out the genetic basis of responses drought stress, we characterised a number accessions. Most accessions belong core collection that was shown maximise diversity captured for given individual thaliana. We measured total leaf area (TLA), Electrolyte Leakage (EL), Relative Water Content (RWC), and Cut Rosette Loss (CRWL) control mild water deficit conditions. A Principal Component Analysis revealed which traits explain...
While Brachypodium distachyon (Brachypodium) is an emerging model for grasses, no expression atlas or gene coexpression network available. Such tools are of high importance to provide insights into the function genes. We present a detailed atlas, capturing in its major organs at different developmental stages. The data were integrated large-scale database ( www.gene2function.de), enabling identification duplicated pathways and conserved processes across 10 plant species, thus allowing...
Abstract The oxidation of monolignols is a required step for lignin polymerization and deposition in cell walls. In dicots, both peroxidases laccases are known to participate this process. Here, we provide evidence that also involved the lignification Brachypodium distachyon, model plant temperate grasses. Transcript quantification data as well situ immunolocalization experiments demonstrated at least two (LACCASE5 LACCASE6) present lignifying tissues. A mutant with misspliced LACCASE5...
Fusarium head blight (FHB) is a cereal disease caused by graminearum, fungus able to produce type B trichothecenes on cereals, including deoxynivalenol (DON), which harmful for humans and animals. Resistance FHB quantitative, the mechanisms underlying resistance are poorly understood. has been related ability conjugate DON into glucosylated form, deoxynivalenol-3-O-glucose (D3G), secondary metabolism UDP-glucosyltransferases (UGTs). However, functional analyses have never performed within...
ABSTRACT To test whether the inhibition of leaf expansion by high evaporative demand is a result hydraulic processes, we have followed both elongation rate (LER) and cell turgor in leaves maize plants either normally watered or water‐saturated soil which resistance at soil–root interface was abolished. Cell measured situ with pressure probe elongating zone first sixth leaves, LERs same were continuously transducers following displacements marks along growing leaves. Both variables displayed...
Abstract Background Drought is a major social and economic problem resulting in huge yield reduction the field. Today's challenge to develop plants with reduced water requirements stable yields fluctuating environmental conditions. Arabidopsis thaliana an excellent model for identifying potential targets plant breeding. tolerance field was successfully conferred crops by transferring genes from this species. While involved genomics programme, which aims identify new responsible response...
Even for easy-to-transform species or genotypes, the creation of transgenic edited plant lines remains a significant bottleneck. Thus, any technical advance that accelerates regeneration and transformation process is welcome. So far, methods to produce Brachypodium distachyon (Bd) transgenics span at least 14 weeks from start tissue culture recovery regenerated plantlets.We have previously shown embryogenic somatic tissues grow in scutellum immature zygotic Bd embryos within 3 days vitro...
Plant somatic embryogenesis (SE) is a natural process of vegetative propagation. It can be induced in tissue cultures to investigate developmental transitions, create transgenic or edited lines, multiply valuable crops. We studied the induction SE scutellum monocots with Brachypodium distachyon as model system. Towards in-depth analysis initiation, we determined earliest stages at which scutellar cells acquired an embryogenic fate, then switched morphogenetic mode regeneration sequence...