Background: Adipose-derived stem cells and other stromal vascular fraction were used more often for cell therapy, even though limitations such as poor retention rate, complicated expensive isolation processes, the use of specific laboratory equipment need to be overcome. Methods: Here, authors developed a novel but simple method generating an injectable mixture native adipose extracellular matrix. It is purely mechanical process in which lipoaspirate processed into matrix/stromal gel. The...

Introduction Glycation products accumulate during aging of slowly renewing tissue, including skin, and are suggested as an important mechanism underlying the skin process. Adipose-derived cells widely used in clinic to treat ischemic diseases enhance wound healing. Interestingly, adipose-derived stem (ASCs) also effective anti-aging therapy, although their effects remains unknown. The purpose present study was examine effect ASCs a D-galactose-induced animal model clarify mechanism....

Abstract Background Adipose-derived stem cells (ASCs) represent the most advantageous choice for soft tissue regeneration. Studies proved recruitment of ASCs post injury was mediated by chemokine CXCL12, but mechanism which CXCL12 is generated after remains unclear. Migrasomes are newly discovered membrane-bound organelles that could deliver spatially and temporally in vivo. In this study, we sought to investigate whether migrasomes participate ASC-mediated Methods Discrepant asymmetrical...

IntroductionAdipose tissue transplantation is one of the standard procedures for soft-tissue augmentation, reconstruction, and rejuvenation. However, it unknown as to how graft survives after transplantation. We thus seek out investigate roles different cellular components in survival graft. Materials & MethodsThe ratios stromal vascular fraction (SVF) from human adipose were evaluated using flow cytometry. Human liposuction aspirates that either mixed with marked SVF cells or PBS...

The major drawback of adipose grafting is its clinical unpredictability, which leads to surgeon and patient dissatisfaction. mechanisms underlying angiogenesis regeneration the graft tissue are still unclear.Mouse was processed using two different methods (fragmental integral) used identify mode graft. Cross-grafting from normal mice transgenic expressing green fluorescent protein observe origin cells during regeneration.Almost all CD31 endothelial new vessels were derived recipient. in...

Background: The apoptosis of mature adipocytes after fat grafting can result in chronic inflammation, absorption, and fibrosis, leading to unpredictable outcomes. Selective elimination may better outcomes a different underlying retention mode. authors previously developed adipocyte–free product, stromal vascular fraction gel, derived from lipoaspirate, which eliminates preserves the fraction. This study investigated regeneration mode gel grafting. Methods: Nude mice were grafted with...

BACKGROUND The clinical outcomes of fat grafting vary and are technique-dependent. Stromal vascular fraction (SVF) gel is a novel, mechanically processed product with high concentrations adipose tissue–derived stem cells other SVF cells. This study evaluated the volumization rejuvenation effects SVF-gel. OBJECTIVE METHODS retrospective, single-center included 126 patients who underwent SVF-gel 78 conventional lipoinjection for various indications from March 2015 to February 2017. Patient...

Progressive hemifacial atrophy (Parry-Romberg disease) is rare and involves all skin layers subcutaneous soft hard tissue. Autologous fat grafting has revolutionized the field of soft-tissue reconstruction augmentation, but long-term maintenance unpredictable. Stromal vascular fraction (SVF)-supplemented cell therapy offers new hope for improving graft survival, with good results, efficacy outcome in clinic are rarely studied using objective data.To compare viability SVF-supplemented grafts...

Our objective was to explore the mechanism of cell-assisted adipose transplantation by using freshly isolated human stromal vascular fraction (SVF) cells and observe dynamic changes graft after transplantation. The SVF from liposuction aspirates, 0.5 ml tissue mixed with 1 × 10(6) or culture medium then injected nude mice subcutaneously. At 1, 4, 7, 14, 30, 60, 90 days transplantation, samples were harvested for 1) general observation retention rate; 2) whole-mount stain; 3) H&E 4)...

Preterm birth (PTB) is a live delivered before 37 weeks of gestation (GW). About one-third PTBs result from the preterm premature rupture membranes (PPROM). Up to present, pathogenic mechanisms underlying PPROM are not clearly understood. Here, we investigated differential expression long chain non-coding RNAs (lncRNAs) in placentas with PPROM, and their possible involvement pathways leading PPROM. A total number 1954, 776, 1050 lncRNAs were identified microarray (group A), which compared...

Tissue engineering chamber (TEC) makes it possible to generate significant amounts of mature, vascularized, stable, and transferable adipose tissue. However, little is known about the role in tissue engineering. Therefore, investigate inflammatory response change mechanotransduction started by TEC after implantation, we placed a unique model on surface groin fat pads rats study expression cytokines development TEC. The number infiltrating cells was counted, vascular endothelial growth factor...

Preterm premature rupture of membranes (PPROM) is responsible for one third all preterm births (PTBs). We have recently demonstrated that long noncoding RNAs (lncRNAs) are differentially expressed in human placentas derived from PPROM, PTB, the (PROM), and full-term birth (FTB), determined major biological pathways involved PPROM.Here, we further investigated relationship lncRNAs, which spontaneous PTB (sPTB) PPROM found to overlap a coding locus, with differential expression transcribed...

Introduction Adipocytes can dedifferentiate into fibroblast-like cells in vitro and thereby acquire proliferation multipotent capacities to participate the repair of various organs tissues. Whether dedifferentiation occurs under physiological or pathological conditions vivo is unknown. Methods A tissue expander was placed inguinal fat pads rats gradually expanded by injection water. Samples were collected at time points, morphological, histological, cytological, ultrastructural, gene...

A novel type of injectable hydrogel is demonstrated as a persistent filler and scaffold for effective repair small facial depressed defects.

Prolonged withdrawal from opioids leads to negative emotions. Kappa opioid receptor (KOR) plays an important role in addiction and affective disorders. However, the underlying mechanism of KOR withdrawal-related depression is still lacking. We found that escitalopram treatment had a limited effect improving symptoms heroin-dependent patients. In mice, we demonstrated prolonged (4 weeks) but not acute (24 h) morphine induced depressive-like behaviors. The number c-Fos positive cells...

Abstract Background Large-area soft tissue defects are challenging to reconstruct. Clinical treatment methods hampered by problems associated with injury the donor site and requirement for multiple surgical procedures. Although advent of decellularized adipose (DAT) offers a new solution these problems, optimal regeneration efficiency cannot be achieved because stiffness DAT altered in vivo adjusting its concentration. This study aimed improve physically altering better repair large-volume...

Evidence suggests that complex interactions among the gut microbiome, metabolic abnormalities, and brain have important etiological therapeutic implications in major depressive disorder (MDD). However, influence of microbiome-gut-brain cross-talk on cognitive impairment MDD remains poorly characterized. We performed serum metabolomic profiling 104 patients with 77 healthy controls (HCs), also fecal metagenomic sequencing a subset these individuals, including 79 60 HCs. The findings were...

BACKGROUND Although fat is transplanted into several layers, including subcutaneous, fat, and muscle there little (clinical) scientific basis for these procedures. OBJECTIVE To determine the optimal recipient layer transplantation. MATERIALS AND METHODS Fat harvested from inguinal pads of green fluorescent protein (GFP) mice was grafted subcutaneous intramuscular planes C57 mice. Specimens collected after 1, 4, 8, 12, 16 weeks were stained with hematoxylin eosin to angiogenesis fibrosis in...

Abstract Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown successful outcome wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF‐gel), an adipose‐derived stem cell adipose native matrix‐enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF‐gel (Gel‐CM) on healing compared with conventional tissue (Adi‐CM) (SVF‐CM). In vitro three CMs keratinocytes fibroblasts was...

Background: Autologous fat grafting is frequently used for volume augmentation and tissue regeneration. The uniform physical biological characteristics of grafts, however, limit their optimal effects in various situations. Subjecting to different mechanical processes results adipose-derived products with distinct components features. present study describes a novel facial strategy, adipose component transplantation (ACT), which yields that can be applied specific injection sites. Methods:...

Abstract Background Perioperative hypothermia is common during liposuction and can lead to delayed recovery, shivering patient discomfort. While electric warming systems are effective, their use may be limited in certain settings. Objectives To evaluate the effectiveness of reflective blankets maintaining core temperature liposuction, compared with no warming. Methods In this randomized controlled trial, 90 patients undergoing elective under general anesthesia were assigned three groups...