A5101953705- Sarcoma Diagnosis and Treatment
- Cardiac tumors and thrombi
- Viral-associated cancers and disorders
- Hepatocellular Carcinoma Treatment and Prognosis
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- Peptidase Inhibition and Analysis
- Radiomics and Machine Learning in Medical Imaging
- Radiation Therapy and Dosimetry
- Cancer Research and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Medical Image Segmentation Techniques
- Neurological disorders and treatments
- Adrenal and Paraganglionic Tumors
- Dermatological and Skeletal Disorders
- Genetic Neurodegenerative Diseases
- Pituitary Gland Disorders and Treatments
- Spondyloarthritis Studies and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Nerve injury and regeneration
- MRI in cancer diagnosis
- Advanced Radiotherapy Techniques
German Cancer Research Center
2020
Universitätsklinikum Erlangen
2016-2018
Friedrich-Alexander-Universität Erlangen-Nürnberg
2016-2018
University Hospital Heidelberg
2014
Heidelberg University
2014
The application of the tumor-specific genomic fusion sequence as noninvasive biomarker for therapy monitoring in Ewing sarcoma (EwS) has been evaluated.EwS xenograft mouse models were used to explore detectability small plasma volumes and correlation EWSR1-FLI1 copy numbers with tumor burden. Furthermore, 234 blood samples from 20 EwS patients analyzed before during multimodal treatment. EWSR1 levels patients' quantified using droplet digital PCR compared calculated MRI or CT imaging...
Glioblastoma (GBM) is a typically lethal type of brain tumor with median survival 15 months postdiagnosis. This negative prognosis prompted the exploration alternative treatment options. In particular, reliance GBM on angiogenesis triggered development anti-VEGF (vascular endothelial growth factor) blocking antibodies such as bevacizumab. Although its application in human only increased progression-free periods but did not improve overall survival, physicians and researchers still utilize...
The aim of the present study is to differentiate subtypes hippocampal sclerosis (HS) using ex vivo ultra-high field magnetic resonance imaging (MRI). Included were 14 surgically resected hippocampi patients with medically intractable temporal lobe epilepsy. histologically categorized into (HS type 1 (n = 10), HS 2 2) and no-HS 2)) subsequently scanned on a preclinical 7T MRI acquiring T2-weighted morphology, relaxometry diffusion tensor imaging. On morphological images, pyramidal cell layer...
ABSTRACT Enthesitis is a key feature of several different rheumatic diseases. Its pathophysiology only partially known due to the lack access human tissue and shortage reliable animal models for enthesitis. Here, we aimed develop model that mimics effector phase enthesitis reliably leads inflammation new bone formation. was induced by local injection monosodium urate (MSU) crystals into metatarsal entheses wild-type (WT) or oxidative-burst-deficient (Ncf1**) mice. Quantitative variables...
The transgenic rat model of Huntington disease expressing a fragment mutant HTT (tgHD rat) has been thoroughly characterized and reproduces hallmark symptoms human adult-onset HD. Pursuing the optimization this for evaluation translational therapeutic approaches, F344 inbred strain was considered as advantageous genetic background expression HD construct. In present study, novel congenic line SPRDtgHD HD, carrying 51 CAG repeats, generated on background. To assess behavioral phenotype,...
Purpose Adamantinomatous craniopharyngiomas (ACP) as benign sellar brain tumors are challenging to treat. In order develop robust in vivo drug testing methodology, the murine orthotopic craniopharyngioma model (PDX) was characterized by magnetic resonance imaging (MRI) and histology xenografts from three patients (ACP1-3). Methods ACP PDX, multiparametric MRI conducted assess morphologic characteristics such contrast-enhancing tumor volume (CETV) well functional parameters dynamic...
Machine learning (ML) algorithms permit the integration of different features into a model to perform classification or regression tasks with an accuracy exceeding its constituents. This protocol describes development ML algorithm predict growth breast cancer bone macrometastases in rat before any abnormalities are observable standard imaging methods. Such can facilitate detection early metastatic disease (i.e., micrometastasis) that is regularly missed during staging examinations. The...
Background: Cell free circulating tumor DNA (ctDNA) has been shown to represent a biomarker in several common cancers of adulthood, taking advantage recurrent point-mutations. We evaluated the use causative genomic fusion sequence as non-invasive marker pediatric EwS.
<p>Tumor engraftment in NMRI nu/nu and NSG mice after s.c. (A) i.v. (B) injection of <i>EWSR1-FLI1</i> positive cell lines EwS type 1 (A673 TC-71) or 2 (VH-64 RD-ES). (C) Localization metastases cells mice. T2-weighted images from ultra high field MRI show the presence organ ex vivo (Lu, Lung; H, Heart; B, Bone; M, Muscle; Li, Liver; K, Kidney; O, Ovary; *, Tumor). H&E stain CD99+ immunostaining tumor infiltration with proliferation rate (60%).</p>
<p>ctDNA levels calculated by normalization of the <i>EWSR1-FLI1</i> signal to single copy gene <i>albumin</i> compared with plasma volume used for DNA extraction. Plasma sample quality was monitored reference (<i>albumin</i>) per volume.</p>
<p>Individual genomic <i>EWSR1-FLI1</i> or <i>EWSR1-ERG</i> fusion sequences. Repeat regions are highlighted in gray.</p>
<p>ctDNA levels calculated by normalization of the <i>EWSR1-FLI1</i> signal to single copy gene <i>albumin</i> compared with plasma volume used for DNA extraction. Plasma sample quality was monitored reference (<i>albumin</i>) per volume.</p>
<p>Individual genomic <i>EWSR1-FLI1</i> or <i>EWSR1-ERG</i> fusion sequences. Repeat regions are highlighted in gray.</p>
<p>Tumor engraftment in NMRI nu/nu and NSG mice after s.c. (A) i.v. (B) injection of <i>EWSR1-FLI1</i> positive cell lines EwS type 1 (A673 TC-71) or 2 (VH-64 RD-ES). (C) Localization metastases cells mice. T2-weighted images from ultra high field MRI show the presence organ ex vivo (Lu, Lung; H, Heart; B, Bone; M, Muscle; Li, Liver; K, Kidney; O, Ovary; *, Tumor). H&E stain CD99+ immunostaining tumor infiltration with proliferation rate (60%).</p>
<div>Abstract<p><b>Purpose:</b> The application of the tumor-specific genomic fusion sequence as noninvasive biomarker for therapy monitoring in Ewing sarcoma (EwS) has been evaluated.</p><p><b>Experimental Design:</b> EwS xenograft mouse models were used to explore detectability small plasma volumes and correlation <i>EWSR1-FLI1</i> copy numbers with tumor burden. Furthermore, 234 blood samples from 20 patients analyzed before...
<div>Abstract<p><b>Purpose:</b> The application of the tumor-specific genomic fusion sequence as noninvasive biomarker for therapy monitoring in Ewing sarcoma (EwS) has been evaluated.</p><p><b>Experimental Design:</b> EwS xenograft mouse models were used to explore detectability small plasma volumes and correlation <i>EWSR1-FLI1</i> copy numbers with tumor burden. Furthermore, 234 blood samples from 20 patients analyzed before...