A5079557280- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Apelin-related biomedical research
- Stress Responses and Cortisol
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuropeptides and Animal Physiology
- Hormonal Regulation and Hypertension
- Neuroscience of respiration and sleep
- Electrolyte and hormonal disorders
- Neuroscience and Neuropharmacology Research
- Nuclear Receptors and Signaling
- Neonatal Respiratory Health Research
- Cannabis and Cannabinoid Research
- Pancreatic function and diabetes
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Birth, Development, and Health
- Pharmacological Receptor Mechanisms and Effects
- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Neuroendocrine Tumor Research Advances
- Neurotransmitter Receptor Influence on Behavior
University of Bristol
2011-2023
Bristol Royal Infirmary
1998-2000
National Institutes of Health
1989-1998
National Institute of Mental Health
1989-1998
University of Pennsylvania
1996
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1996
National Heart Lung and Blood Institute
1995-1996
Mayo Clinic
1993
Yale University
1992
Monash Medical Centre
1984-1986
Abstract Cannabinoid receptor mRNA was localized in adult rat brain by 35 S‐tailed oligonucleotide probes and situ hybridization histochemistry. Labelling is described as uniform or non‐uniform depending on the relative intensities of individual cells expressing cannabinoid within a given region nucleus. Uniform labelling found hypothalamus, thalamus, basal ganglia, cerebellum brainstem. Non‐uniform that resulted from presence displaying two easily distinguishable signals observed several...
The Concise Guide to PHARMACOLOGY 2021/22 is the fifth in this series of biennial publications. provides concise overviews, mostly tabular format, key properties nearly 1900 human drug targets with an emphasis on selective pharmacology (where available), plus links open access knowledgebase source and their ligands ( www.guidetopharmacology.org ), which more detailed views target ligand properties. Although constitutes over 500 pages, material presented substantially reduced compared...
Recently, the G protein-coupled receptor GPR30 has been identified as a novel oestrogen (ER). The distribution of thus far mapped only in rat central nervous system. This study was undertaken to map mouse brain and rodent peripheral tissues. Immunohistochemistry using an antibody against revealed high levels immunoreactivity (ir) forebrain (e.g. cortex, hypothalamus hippocampus), specific nuclei midbrain pontine locus coeruleus) trigeminal cerebellum Purkinje layer hindbrain adult brain. In...
[Arg8]vasopressin (AVP) stimulates adrenocorticotropic hormone release from the anterior pituitary by acting on V1b AVP receptor. This receptor can be distinguished vascular/hepatic V1a and renal V2 receptors its differential binding affinities for structural analogous of AVP. Recent studies have shown that cloned are structurally related. We isolated a clone encoding rat cDNA library using polymerase chain reaction (PCR)-based methodology. The is protein 421 amino acids has 37-50% identity...
The comparative distributions of the vasopressin V1b receptor (V1bR) and oxytocin (OTR) messenger RNAs (mRNAs) are described in male rat brain using situ hybridization histochemistry. V1bR transcripts were present forebrain hypothalamus less abundant mid- hindbrain regions, similar to gradient observed with OTR transcripts. Microscopic analyses indicated that expressing cells typically demonstrated morphology neurons confirmed gene expression regions including olfactory bulb, supraoptic,...
Vasopressin V1a receptor (V1aR) transcripts were localized in brain, pineal, and superficial brain vascular tissues of adult male rats using hybridization histochemistry an [35S]riboprobe complementary to the messenger ribonucleic acid (mRNA) encoding fifth midseventh transmembrane regions receptor. V1aR mRNA was extensively distributed throughout expressed 1) cells granule cell layers main olfactory bulb, hippocampal dentate gyrus, cerebellum; 2) numerous anatomically distinct nuclei; 3)...
Biological effects of vasopressin (VP) are mediated by four different receptors, two which (the V1a and the oxytocin receptors) have been well characterized in rodent brain, suggesting that these main receptors responsible for central VP. However, transcripts V1b VP receptor (V1bR) detected throughout rat brain RT-PCR situ hybridization, indicating V1bR adds to population receptors. Because there no specific ligands V1bR, protein itself has difficult visualize. In present study, distribution...
The hepatic, vascular-type (V1aR) and the renal, antidiuretic-type (V2R) vasopressin receptor cDNAs were recently cloned from rat liver kidney libraries, respectively. DNA fragments containing region encoding putative 5/6 transmembrane loops of these receptors subcloned, separately, into RNA polymerase promoter-containing vectors which 35S-labeled sense antisense riboprobes synthesized. In situ hybridization histochemistry showed high levels V1aR transcripts in renal medulla among vascular...
We have previously demonstrated low levels of immunoreactive (ir)-beta-endorphin (beta-EP) and ir-ACTH in a subpopulation mouse spleen macrophages, which is consistent with an involvement opioid peptides modulation immune responses. Gel chromatography studies suggested the presence approximately 3.5,000-molecular weight (mol wt) species, putatively beta-EP, 11.5,000-mol-wt beta-lipotropin, higher molecular species (putative beta-EP precursor, pro-opiomelanocortin (POMC). In this study we...
Using radioimmunoassay and immunofluorescence with antibodies to beta-endorphin (beta EP) ACTH, we have shown that a subpopulation of mouse spleen cells, expressing Mac-1, marker macrophage differentiation, contains immunoreactive (ir)-beta EP, ir-ACTH, smaller amounts presumptive higher molecular weight forms both. Neither nonadherent nor adherent or cells from peripheral blood, contained detectable levels these peptides. These findings suggest beta EP ACTH may be synthesized in...
The G protein-coupled apelin receptor (APJ) binds the endogenous peptide and has been shown to have roles in many physiological systems. Thus far, distribution studies predominantly conducted rat there is limited knowledge of cellular APJ mouse or human tissues. As recent functional knock-out mice (APJ KO), this study we undertook characterize mRNA I(125)[Pyr(1)]apelin-13 binding site tissues enable correlation with function. We utilized situ hybridization histochemistry (ISHH) using...
Corticotropin releasing hormone (CRH), a major regulator of pituitary ACTH secretion, also acts as neurotransmitter in the brain. To determine whether CRH is involved regulation hypothalamic function during stress, receptor binding and mRNA levels were studied hypothalamus rats subjected to different stress paradigms: immobilization, physical-psychological model; water deprivation 2% saline intake, osmotic models; i.p. hypertonic injection, combined model. In agreement with distribution...
The ontogenic expression of mRNAs encoding the V1a and V2 vasopressin receptors (V1aR V2R) was visualized in liver kidney embryonic, developing, adult rats using situ hybridization histochemistry. Transcripts were detected at 16 19 days gestational age kidney, with V1aR mRNA predominating developing cortex V2R medulla. transcripts 1-day-old kidneys vascular elements, cells medullary collecting ducts, over mesangial deep glomeruli, consistent a role for regulating cellular growth. Expression...
The role of arginine vasopressin (Avp) as an ACTH secretagogue is mediated by the Avp 1b receptor (Avpr1b) found on anterior pituitary corticotropes. also potentiates actions CRH (Crh) and appears to be important mediator hypothalamic-pituitary-adrenal axis response chronic stress. To investigate in stress, we measured plasma corticosterone (CORT) levels Avpr1b knockout (KO) mice wild-type controls two acute (restraint insulin administration) one form (daily restraint for 14 d) No...
The apelinergic system, comprised of apelin and its G protein-coupled receptor (APJ; APLNR as given in MGI Database), is expressed within key regions the central nervous system associated with arginine vasopressin (AVP) synthesis release well structures involved control drinking behaviour, including magnocellular neurones hypothalamus, circumventricular organs, pituitary gland. This localisation indicative a possible functional role fluid homeostasis. We investigated for APJ regulation...
Two full‐length cDNA clones encoding α‐ and β‐subunits of a GABA A receptor have been isolated from rat cerebral cortex library. The mature α‐subunit protein consists 428 amino acids with calculated M r 48680. This is highly homologous (∼99% acid identity) the bovine brain α 1 ‐subunit [(1988) Nature 335, 76–79]. β‐subunit 448 polypeptide shares ∼ 80% identity previously characterized [(1987) 328, 221–227]. Co‐expression cloned DNA in Xenopus oocytes produces functional ion channel...