A5073478356- Hemoglobin structure and function
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Neonatal Health and Biochemistry
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Erythrocyte Function and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Catalysis and Hydrodesulfurization Studies
- Photoreceptor and optogenetics research
- RNA modifications and cancer
- Biofuel production and bioconversion
- Wastewater Treatment and Nitrogen Removal
- Microbial bioremediation and biosurfactants
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Pesticide and Herbicide Environmental Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Invertebrate Immune Response Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- RNA Research and Splicing
Illinois Institute of Technology
2015-2025
The Ohio State University
2022
Yale University
1974-2003
Kyoto University
1998
Kraft Heinz (United States)
1994
Indiana University Bloomington
1984-1985
The activity of ribonuclease P on precursor tRNA substrates from Escherichia coli can be abolished by pretreatment this enzyme with micrococcal nuclease or pancreatic A, as well proteases and thermal denaturation. Highly purified RNase exhibits one prominent RNA polypeptide component when examined in polyacrylamide gels containing sodium dodecyl sulfate. buoyant density CsCl P, 1.71 g/ml, is characteristic a protein-RNA complex. inhibited various molecules. presence discrete appears to...
The obligate aerobic bacterium, Vitreoscilla, synthesizes elevated quantities of a homodimeric hemoglobin (VHb) under hypoxic growth conditions. Expression VHb in heterologous hosts often enhances and product formation. A role facilitating oxygen transfer to the respiratory membranes is one explanation its cellular function. Immunogold labeling both Vitreoscilla recombinant Escherichia coli bearing gene clearly indicated that has cytoplasmic (not periplasmic) localization concentrated near...
The bacterium, Vitreoscilla, can induce the synthesis of a homodimeric hemoglobin under hypoxic conditions. Expression VHb in heterologous bacteria often enhances growth and increases yields recombinant proteins production antibiotics, especially oxygen-limiting There is evidence that interacts with bacterial respiratory membranes cytochrome bo proteoliposomes. We have examined whether there are binding sites for on cytochrome, using yeast two-hybrid system as bait testing every Vitreoscilla...
Abstract The characteristics of growth and synthesis plasmid‐encoded protein were studied for strains recombinant E. coli JM103 which carried the β‐lactamase gene on plasmids different sizes. used included vector pUC8 its derivatives containing varying‐sized inserts Drosophila DNA (not expressed in ). Luria broth (LB) a minimal medium (M9) supplemented some cases with additional inorganic phosphate as media. There was no evidence segregational instability these experiments, where antibiotic...
RNase P can cleave in vitro a bacteriophage phi80-induced RNA which is 62 nucleotides long [M3 RNA, G. Pieczenik et al. (1972) Arch. Biochem. Biophys. 152, 152-165] to yield two specific fragments 25 and 37 long. As the case for another substrate of P; precursor Escherichia coli 4.5S cleavage site M3 at end double-stranded region immediately adjacent single-stranded segment. Similar nucleotide sequences span both substrates. These other features reaction with are different from some aspects...
In previous work from our laboratories a synthetic gene encoding peptide ("Sulpeptide 1" or "S1") with high proportion of methionine and cysteine residues had been designed to act as sulfur sink was inserted into the dsz (desulfurization) operon Rhodococcus erythropolis IGTS8. described here this construct (dszAS1BC) intact (dszABC) cloned vector pRESX under control (Rhodococcus) kstD promoter were transformed desulfurization-negative strain CW25 qingshengii. The resulting strains...
Abstract The gene ( vgb ) encoding the hemoglobin (VHb) of Vitreoscilla sp. was cloned into a broad host range vector and stably transformed Burkholderia (formerly Pseudomonas strain DNT, which is able to degrade metabolize 2,4‐dinitrotoluene (DNT). Vgb maintained expressed in functional form this recombinant (YV1). When growth YV1, both tryptic soy broth minimal salts containing DNT yeast extract, compared with that untransformed strain, YV1 grew significantly better on cell mass basis A...
Ethanol production from whey powder was investigated by using free as well alginate immobilized E. coli and expressing Vitreoscilla hemoglobin (VHb) in both shake flask fermenter cultures. Media with varying levels of (lactose contents 3%, 5%, 8% or 15%) yeast extract (0.3% 0.5%) were evaluated fermentation times 48–96 h. Immobilization VHb expression resulted higher ethanol all media; the increases ranged 2% to 89% for immobilization 182% expression. It determined that growth medium...
As the source of first reported class non-mammalian hemoglobin, Vitreoscilla sp. C1 is a historically important microorganism that has offered clues to understanding how bacteria can thrive at low oxygen tension, with potential applications wastewater and sludge bioengineering. However, processes enable this bacterium in such environments remain unclear. In study, we analyzed published genome predict core metabolic pathways used by support cell growth under hypoxic conditions, compared them...