A5008923633- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- Counseling Practices and Supervision
- Genomics and Phylogenetic Studies
- Medicinal Plants and Neuroprotection
- Catalysis and Hydrodesulfurization Studies
- Single-cell and spatial transcriptomics
- Primary Care and Health Outcomes
- Peptidase Inhibition and Analysis
- Genomics and Rare Diseases
- Plant and Fungal Interactions Research
- Monoclonal and Polyclonal Antibodies Research
- Quinazolinone synthesis and applications
- Genetic Associations and Epidemiology
- Mast cells and histamine
- Microbial Metabolic Engineering and Bioproduction
- T-cell and B-cell Immunology
- Psychotherapy Techniques and Applications
- Medical Imaging and Pathology Studies
- Parasitic Infections and Diagnostics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cholinesterase and Neurodegenerative Diseases
- Tuberculosis Research and Epidemiology
Stanford University
2021-2024
NorthShore University HealthSystem
2018-2021
Palo Alto University
2021
University of Chicago
2018-2020
Université de Sherbrooke
2019
Genomic Health (United States)
2018
Illinois Institute of Technology
2014-2017
California Institute of Technology
2008-2014
Goddard Space Flight Center
2008-2013
University of Florida Health Science Center
1994-1998
Abstract INTRODUCTION Alzheimer's disease (AD) is the predominant dementia globally, with heterogeneous presentation and penetrance of clinical symptoms, variable presence mixed pathologies, potential subtypes, numerous associated endophenotypes. Beyond difficulty designing treatments that address core pathological characteristics disease, therapeutic development challenged by uncertainty which endophenotypic areas specific targets implicated those endophenotypes to prioritize for further...
Abstract GATA-3 expression is crucial for T cell development and peaks during commitment to the lineage, midway through CD4−CD8− (double-negative [DN]) stages 1–3. We used RNA interference conditional deletion reduce protein acutely at specific points differentiation in vitro. Even moderate reduction killed DN1 cells, delayed progression DN2 stage, skewed gene regulation, blocked appearance of DN3 phenotype. Although a Bcl-2 transgene rescued survival improved generation, it did not restore...
During previous cooperative numerical taxonomic studies of slowly growing mycobacteria, the International Working Group on Mycobacterial Taxonomy described a number strains whose status was ambiguous. A new study DNA, RNA, and proteins from 66 these organisms performed to correlate their properties with phenotypic clustering behavior; results this permitted 51 studied be assigned known species. The methods used characterize semantides included nucleotide sequencing assessment levels...
The relationship of C1 -inhibitor (C1 -INH) concentration and apparent functional activity was investigated in 111 plasma samples from 21 patients with the common form hereditary angioedema (HAE). Functional -INH analyzed by means a modified version immunodiffusion assay. Down to C1-INH level approximately 0.075 g/1 (38% normal) functions were found within normal range, while below this adequacy could no longer be ascertained. When C4 concentrations, considered reflect C1-INH, related C1-...
The sub-3 Mbp genomes from microsporidian species of the Encephalitozoon genus are smallest known among eukaryotes and paragons genomic reduction compaction in parasites. However, their diminutive stature is not characteristic all Microsporidia, whose genome sizes vary by an order magnitude. This large variability suggests that different evolutionary forces applied on group as a whole. In this study, we have compared codon usage bias (CUB) between eight taxonomically distinct genomes:...
Abstract INTRODUCTION Tropomyosin related kinase B (TrkB) and C (TrkC) receptor signaling promotes synaptic plasticity interacts with pathways affected by amyloid beta (Aβ) toxicity. Upregulating TrkB/C could reduce Alzheimer's disease (AD)‐related degenerative signaling, memory loss, dysfunction. METHODS PTX‐BD10‐2 (BD10‐2), a small molecule partial agonist, was orally administered to aged London/Swedish‐APP mutant mice (APP L/S ) wild‐type controls. Effects on hippocampal long‐term...
In previous work from our laboratories a synthetic gene encoding peptide ("Sulpeptide 1" or "S1") with high proportion of methionine and cysteine residues had been designed to act as sulfur sink was inserted into the dsz (desulfurization) operon Rhodococcus erythropolis IGTS8. described here this construct (dszAS1BC) intact (dszABC) cloned vector pRESX under control (Rhodococcus) kstD promoter were transformed desulfurization-negative strain CW25 qingshengii. The resulting strains...
The availability of amyloid beta (Aβ) targeting therapies for Alzheimer's disease (AD) is increasing the demand scalable biomarkers that are sensitive to early cerebral Aβ accumulation.
A cooperative study was conducted by the International Working Group on Mycobacterial Taxonomy to correlate agglutination serovar designations of Mycobacterium avium, intracellulare, and scrofulaceum strains with species ascriptions these organisms according molecular criteria cultural properties assess reproducibility determinations for a set 63 reference species. Among criteria, level agreement between results obtained nucleic acid probes T-catalase serology 94% M. avium intracellulare....
The biologic basis for the elevated histochemical reduction of nitroblue tetrazolium dye (NBT) in neutrophils from patients with acute bacterial infection or polycythemia vera was studied. A precipitin reaction followed mixing NBT heparin. reduced after phagocytosis this complex (H-NBT) by polymorphonuclear leukocytes (PMNs). Ingestion required divalent cations and facilitated presence complement. H-NBT incubated normal but not C2-deficient human serum converted native C3 to its inactive form.
Huntington's disease (HD) is caused by an expansion of the CAG repeat in huntingtin gene leading to preferential neurodegeneration striatum. Disease-modifying treatments are not yet available HD patients and their development would be facilitated translatable pharmacodynamic biomarkers. Multi-modal magnetic resonance imaging (MRI) plasma cytokines have been suggested as onset/progression biomarkers, but ability detect treatment efficacy understudied. This study used R6/2 mouse model assess...
We investigated the effects of thromboxane synthetase inhibitor 7-(1-imidazolyl)heptanoic acid (7-IHA) and fatty cyclooxygenase inhibitors indomethacin or ibuprofen in treatment fecal peritonitis rat. The gentamicin alone combination with reduction arachidonic metabolism by either essential deficiency was also investigated. 7-IHA (60 mg/kg), administered i.p. 30 min before instillation a suspension, significantly reduced plasma levels immunoreactive (i) TxB2 from 1066 +/- 194 pg/ml (N = 14)...
The LCRD will demonstrate optical communications relay services between a geosynchronous satellite and Earth over an extended period, thereby gain the knowledge experience base that enable NASA to design, procure, operate cost-effective future systems networks. is next step in eventually providing service on Next Generation Tracking Data Relay Satellites (TDRS). some technologies, concepts of operations, advanced networking technologies applicable Deep Space missions. In this paper we...
Clostridium sporogenes PA 3679 is a non-toxic endospore former that widely used as surrogate for botulinum by the food processing industry to validate thermal strategies. produces spores of exceptionally high heat resistance without neurotoxins, permitting use in inoculated pack studies while ensuring safety facilities. To identify genes associated with this resistance, genomes C. isolates were compared several other strains. The most significant difference was acquisition second spoVA...
ABSTRACT Clostridium sporogenes PA 3679 is a nonpathogenic, nontoxic model organism for proteolytic botulinum used in the validation of conventional thermal food processes due to its ability produce highly heat-resistant endospores. Because public safety importance, uncertain taxonomic classification and genetic diversity are concerns. Therefore, isolates C. were obtained from various sources characterized using pulsed-field gel electrophoresis (PFGE) whole-genome sequencing. The...
Microglia are the primary innate immune cell type in brain that have been implicated pathogenesis of several neurodegenerative and neuropsychiatric disorders, most notably Alzheimer's disease (AD) schizophrenia. generated from human induced pluripotent stem cells (hiPSCs) represent a promising vitro cellular model for studying neuroimmune interactions involved these disorders. Among methods generating -hiPSC-derived microglia (iMG) - varying duration resultant purity recent protocol by...
Rhogocytes are morphologically distinct cells distributed throughout connective tissues of crustaceans and molluscs. Using light microscopy, rhogocytes the vetigastropod Megathura crenulata were identified by their ovoid shape, cytoplasm filled with spherical inclusions which contained lysosomal enzymes, based on uptake neutral red staining LysoTracker dye. most abundant in digestive gland (2,824 rhogocytes/mm2), followed tissue layer surrounding middle posterior esophagus intestine (1,431...
Alzheimer's disease (AD) is a pervasive neurodegenerative disorder that leads to memory and behavior impairment severe enough interfere with daily life activities. Understanding this pathogenesis can drive the development of new targets strategies prevent treat AD. Recent advances in high-throughput single-cell RNA sequencing technology (scRNA-seq) have enabled generation massive amounts transcriptomic data at level provided remarkable insights into understanding molecular disease. In study,...