A5057089904- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Radioactive element chemistry and processing
- Soil and Unsaturated Flow
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Groundwater flow and contamination studies
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Epigenetics and DNA Methylation
- Nuclear materials and radiation effects
- Chemical Synthesis and Characterization
- Diabetes and associated disorders
- Systemic Lupus Erythematosus Research
- Helminth infection and control
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Groundwater and Isotope Geochemistry
- Radioactive contamination and transfer
- Acute Lymphoblastic Leukemia research
- Glass properties and applications
- Analytical Chemistry and Sensors
- Geochemistry and Elemental Analysis
- Coral and Marine Ecosystems Studies
- Coal and Its By-products
California Institute of Technology
2008-2021
Japan Atomic Energy Agency
1993-2006
Health First
2006
J-Power (Japan)
1994-1998
University of Florida
1996-1997
Brigham and Women's Hospital
1988-1991
Harvard University Press
1989
Oregon State University
1983-1987
Virginia Commonwealth University
1985
Because TNF and IL-1 can initiate immunologic inflammatory events alone or synergistically, a local increase in the levels of one both these cytokines vivo may cause irreparable tissue damage. The purpose this study was to evaluate beta gene expression kidneys MRL-Ipr mice with autoimmune lupus nephritis. mRNA detected renal cortex but not present normal congenic MRL-++ C3H/FeJ mice. nephritis expressed higher amounts compared prior disease. In addition, freshly isolated, unstimulated...
TNF and IL-1 are potent immunologic inflammatory cytokines. We have previously reported increased levels of mRNA for alpha beta in MRL-lpr mice with lupus nephritis. To determine whether the a more general feature nephritis we studied cytokine gene expression female NZB x NZW F1 (NZB/W) by Northern blot analysis. Enhanced steady state beta, but not alpha, were detected renal cortices animals administration or would accelerate injury mortality, injected murine rTNF rIL-1 i.p. into NZB/W...
Abstract T cell development is marked by the loss of alternative lineage choices accompanying specification and commitment to lineage. Commitment occurs between CD4 CD8 double-negative (DN) 2 DN3 stages in mouse early cells. To determine gene regulatory changes that accompany commitment, we sought distinguish characterize earliest committed wild-type DN adult thymocytes. A transitional population, defined first downregulation surface c-Kit expression, was found have lost ability...
Abstract IL-1 is a pleiotropic factor encoded for by at least two genes, alpha and beta, capable of eliciting broad set immunologic inflammatory events. MRL/MP-lpr (MRL-lpr) mice are an appealing model studies renal injury inasmuch as disease in this strain spontaneous, rapid, predictable, regulated the lpr gene. Infiltration macrophages proliferation glomerular mesangial cells prominent features disease. Because both can synthesize IL-1, purpose study was to determine whether enhanced gene...
Cell fate decisions occur through the switch-like, irreversible activation of fate-specifying genes. These events are often assumed to be tightly coupled changes in upstream transcription factors, but could also constrained by cis-epigenetic mechanisms at individual gene loci. Here, we studied Bcl11b, which controls T-cell commitment. To disentangle cis and trans effects, generated mice where two Bcl11b copies tagged with distinguishable fluorescent proteins. Quantitative live microscopy...
PU.1 is essential for early stages of mouse T cell development but antagonizes it if expressed constitutively. Two separable mechanisms are involved: attenuation and diversion. Dysregulated expression inhibits pro-T survival, proliferation, passage through β-selection by blocking transcription factors, signaling molecules, Rag gene expression, which a rearranged antigen receptor transgene cannot rescue. However, Bcl2 transgenic cells protected from this may even undergo β-selection, as shown...
Choice of a T lymphoid fate by hematopoietic progenitor cells depends on sustained Notch–Delta signaling combined with tightly regulated activities multiple transcription factors. To dissect the regulatory network connections that mediate this process, we have used high-resolution analysis gene expression trajectories from beginning to end specification, tests short-term Notch dependence these changes, and analyses effects overexpression two essential factors, namely PU.1 GATA-3....
We propose a new multiclass weighted loss function for instance segmentation of cluttered cells. are primarily motivated by the need developmental biologists to quantify and model behavior blood T-cells which might help us in understanding their regulation mechanisms ultimately researchers quest developing an effective immuno-therapy cancer treatment. Segmenting individual touching cells regions is challenging as feature distribution on shared borders cell foreground similar thus...
Abstract Although the promoter/enhancer of IL-2 gene mediates inducible reporter expression in vitro, it cannot drive consistent transgenic mice. The location and existence any regulatory elements that could open locus vivo have remained unknown, preventing analysis regulation developmental contexts. In this study, we report identification such a region, marked by novel DNase-hypersensitive sites upstream murine promoter unstimulated stimulated T cells. Inclusion most these an 8.4-kb green...
Abstract A transgene with 8.4-kb of regulatory sequence from the murine IL-2 gene drives consistent expression a green fluorescent protein (GFP) reporter in all cell types that normally express IL-2. However, quantitative analysis this shows different T subsets within same mouse show divergent abilities to as compared endogenous genes. TCRγδ cells, well αβTCR-NKT exhibit higher vivo levels than TCRαβ cells. This deviates patterns normal and an IL-2-GFP knock-in. Peripheral cells accumulate...