A5100332174- Genetic Associations and Epidemiology
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- Genetics and Neurodevelopmental Disorders
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Cryospheric studies and observations
- Genetics, Aging, and Longevity in Model Organisms
- Alzheimer's disease research and treatments
- Regional Development and Environment
- Landslides and related hazards
- Neuroscience and Neuropharmacology Research
- Climate change and permafrost
- Clusterin in disease pathology
- Receptor Mechanisms and Signaling
- Bioinformatics and Genomic Networks
- Educational Reforms and Innovations
- Winter Sports Injuries and Performance
- Genomics and Rare Diseases
- Medical Research and Treatments
- Neural dynamics and brain function
- Digital Media and Visual Art
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2025
Wenzhou Medical University
2025
State Forestry and Grassland Administration
2025
Guangxi University of Science and Technology
2025
Universidad del Noreste
2025
NorthShore University HealthSystem
2017-2024
East China Jiaotong University
2024
Chinese Academy for Environmental Planning
2023
Minzu University of China
2023
University of Chicago
2020-2023
The mixed linear model has been widely used in genome-wide association studies (GWAS), but its application to multi-locus GWAS analysis not explored and assessed. Here, we implemented a fast random-SNP-effect EMMA (FASTmrEMMA) for GWAS. is built on random single nucleotide polymorphism (SNP) effects new algorithm. This algorithm whitens the covariance matrix of polygenic K environmental noise, specifies number nonzero eigenvalues as one. first chooses all putative quantitative trait...
Osteoarthritis (OA) is a prevalent degenerative disease that lacks effective therapy. Oxidative stress one of the major factors contributing to OA; however, treatments targeting oxidative are still lacking. In current study, we established an stress-induced cell death model in chondrocytes vitro and screened drugs may suppress death. Ethyl gallate (EG) was identified as most potent drug against out more than 600 natural product library. Application without appropriate delivery system for OA...
Effects of allele-specific open chromatin Genetic variants in noncoding regions the genome may underlie development disease. However, we are just beginning to tease apart function such associated with neuropsychiatric Using five types neural progenitor cells derived from 20 human induced pluripotent stem cell lines, Zhang et al. looked at (ASoC) variants. Many ASoC overlapped genomic elements, as transcription factor binding sites, and loci identified genome-wide association studies for...
Applying tissue-specific deconvolution of transcriptional networks to identify their master regulators (MRs) in neuropsychiatric disorders has been largely unexplored. Here, using two schizophrenia (SCZ) case-control RNA-seq datasets, one on postmortem dorsolateral prefrontal cortex (DLPFC) and another cultured olfactory neuroepithelium, we deconvolved the identified TCF4 as a top candidate MR that may be dysregulated SCZ. We validated through enrichment analysis TCF4-binding sites induced...
The mechanistic tie between genome-wide association study (GWAS)-implicated risk variants and disease-relevant cellular phenotypes remains largely unknown. Here, using human induced pluripotent stem cell (hiPSC)-derived neurons as a neurodevelopmental model, we identify multiple schizophrenia (SZ) that display allele-specific open chromatin (ASoC) are likely to be functional. Editing the strongest ASoC SNP, rs2027349, near
ABSTRACT Oenococcus oeni, the only species of lactic acid bacteria capable fully completing malolactic fermentation under challenging wine conditions, continues to intrigue researchers owing its remarkable adaptability, particularly in combating stress. However, mechanism underlying superior adaptation stresses still remains elusive due lack viable genetic manipulation tools for this species. In study, we conducted genomic sequencing and resistance phenotype analysis 255 O. oeni isolates...
Schizophrenia (SCZ) is a debilitating psychiatric disorder with large genetic contribution; however, its neurodevelopmental substrates remain largely unknown. Modeling pathogenic processes in SCZ using human induced pluripotent stem cell-derived neurons (iNs) has emerged as promising strategy. Copy number variants confer high risk for SCZ, duplication of the 16p11.2 locus increasing 14.5-fold.
Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase cytoplasm (GAPCs) have remained little investigated subjected to adverse environmental conditions. In this study, we cloned characterized GAPC isoform TaGAPC2 wheat. Over-expression TaGApC2-6D Arabidopsis led enhanced root length, reduced reactive oxygen species (ROS)...
Schizophrenia (SZ) is a common and debilitating psychiatric disorder with limited effective treatment options. Although highly heritable, risk for this polygenic depends on the complex interplay of hundreds rare variants. Translating growing list genetic loci significantly associated disease into medically actionable information remains an important challenge. Thus, establishing platforms which to validate impact variants in cell-type-specific donor-dependent contexts critical. Towards this,...
<title>Abstract</title> Despite genome-wide association studies of late-onset Alzheimer’s disease (LOAD) having identified many genetic risk loci 1-6, the underlying mechanisms remain largely unknown. Determining causal variants and their LOAD-relevant cellular phenotypes has been a challenge. Leveraging our approach for identifying functional GWAS showing allele-specific open chromatin (ASoC) 7, we systematically putative LOAD in human induced pluripotent stem cells (iPSC)-derived neurons,...
When pouring concrete overhead, a pump truck boom’s vibration has big effect on how accurately the is poured. This especially true during fixed-point pouring, where likely to cause position deviate, which lowers quality of construction. It difficult forecast dynamic reaction boom in construction setting because constantly shifting external factors (wind speed, pipeline stress pumping, etc.), makes it guarantee casting accuracy. study suggests non-random analysis technique for booms based...
Abstract Despite hundreds of genetic risk loci identified for neuropsychiatric disorders (NPD), most causal variants/genes remain unknown. A major hurdle is that disease variants may act in specific biological contexts, e.g., during neuronal activation, which difficult to study vivo at the population level. Here, we conducted a single-cell multiomics activation (stimulation) human iPSC-induced excitatory and inhibitory neurons from 100 donors, uncovered abundant stimulation-specific NPD. We...
This study investigates the construction of female identity through multimodal (visual-textual) resources in Xiaohongshu digital advertising, focusing on YSLs 1966 lipstick advertising video. Integrating Multimodal Discourse Analysis (MDA), Gender Performativity Theory, and Russells Circumplex Model Emotion, a mixed-methods experiment engaged 10 participants 30 valid questionnaires results with interview to compare three video versions: image-only, text-only, full-modality. Results...
Abstract Background Genome-wide association studies (GWAS) of Alzheimer’s disease (AD) have identified a plethora risk loci. However, the variants/genes and underlying mechanisms not been extensively studied. Methods Bulk ATAC-seq was performed in induced pluripotent stem cells (iPSCs) differentiated various brain cell types to identify allele-specific open chromatin (ASoC) SNPs. CRISPR-Cas9 editing generated isogenic pairs, which were then into glutamatergic neurons (iGlut). Transcriptomic...
Cell type-specific pathway-based polygenic risk scores (PRSs) may better inform disease biology and improve the precision of PRS-based clinical prediction. For microRNA-137 (MIR137), a leading neuropsychiatric gene post-transcriptional master regulator, we conducted cell set PRS analysis in both European Han Chinese schizophrenia (SZ) samples. We found that neuronal MIR137-target genes explains SZ than derived from iPSC or reported sets showing MIR137-altered expression. Compared with whole...
Abstract Although the predictive effect of emotion on language achievement has been substantially established, little is known about whether could, in turn, shape a constellation emotions second language/L2 learning, especially field languages other than English. Given this, grounded control-value theory, this tentative study aims to fill gap by investigating (enjoyment, boredom, and anxiety) digging into mediating relationships between them underlying L2 Chinese learning mechanism through...
Chromatin accessibility to transcription factors (TFs) strongly influences gene and cell differentiation. However, a mechanistic understanding of the transcriptional control during neuronal differentiation human induced pluripotent stem cells (hiPSCs), promising cellular model for mental disorders, remains elusive. Here, we carried out additional analyses on our recently published open chromatin regions (OCRs) profiling at different stages hiPSC We found that dynamic changes OCR highlighted...
Microglia are the primary innate immune cell type in brain that have been implicated pathogenesis of several neurodegenerative and neuropsychiatric disorders, most notably Alzheimer's disease (AD) schizophrenia. generated from human induced pluripotent stem cells (hiPSCs) represent a promising vitro cellular model for studying neuroimmune interactions involved these disorders. Among methods generating -hiPSC-derived microglia (iMG) - varying duration resultant purity recent protocol by...
Summary Genome-wide association studies (GWAS) of Alzheimer’s disease (AD) have identified a plethora risk loci. However, the variants/genes and underlying mechanisms remain largely unknown. For strong AD-associated locus near Clusterin ( CLU ), we tied an AD protective allele to role neuronal in promoting neuron excitability through lipid-mediated neuron-glia communication. We putative causal SNP that impacts neuron-specific chromatin accessibility transcription-factor(s), with upregulating...
Abstract Efficient, accurate molecular characterization of genetically modified (GM) organisms is challenging, especially for those transgenic events transferred with genes/elements recipient species. Herein, we decipher the comprehensive one novel GM rice event G281 which was native promoters and an RNA interference (RNAi) expression cassette using paired-end whole genome sequencing (PE-WGS) TranSeq approach. Our results show that transgenes integrate at chromosome 3 locus 16,439,674...