A5092640207- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Biochemical and Molecular Research
- Pancreatic and Hepatic Oncology Research
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- Dialysis and Renal Disease Management
KU Leuven
2023-2024
The genomic landscape of colorectal cancer (CRC) is shaped by inactivating mutations in tumour suppressors such as APC, and oncogenic mutant KRAS. Here we used genetically engineered mouse models, multimodal mass spectrometry-based metabolomics to study the impact common genetic drivers CRC on metabolic intestine. We show that untargeted profiling can be applied stratify intestinal tissues according underlying alterations, use spectrometry imaging identify tumour, stromal normal adjacent...
Abstract Defining the number and abundance of different cell types in tissues is important for understanding disease mechanisms as well diagnostic prognostic purposes. Typically, this achieved by immunohistological analyses, sorting, or single-cell RNA-sequencing. Alternatively, cell-specific DNA methylome information can be leveraged to deconvolve fractions from a bulk mixture. However, comprehensive benchmarking deconvolution methods modalities was not yet performed. Here we evaluate 16...
Maternal dietary insufficiencies can reshape the fetal epigenome during gestation, contributing to birth defects and developmental disorders. Vitamin C (VitC) is a critical co-factor for Ten-Eleven-Translocation (TET) DNA demethylases, but impact of its deficiency on embryonic development has gone largely unappreciated. Here, we show that maternal VitC in L-gulonolactone oxidase ( Gulo )-deficient mice, which like humans are unable synthesize VitC, cause highly penetrant delays malformations...
Abstract Background and Aims Patients treated with hemodialysis demonstrate a significant cognitive decline, likely explained by cerebrovascular disease hypoperfusion. Despite the morbidity mortality associated, no method exists to detect subclinical cerebral injury. This prevents early diagnosis precludes mitigation strategies. Following cell death, free DNA (cfDNA) is released into peripheral blood. Since methylation cell-type specific, cfDNA analysis can reveal cellular origin of specific...
<title>Abstract</title> Defining the number and abundance of different cell types in tissues is important for understanding disease mechanisms as well diagnostic prognostic purposes. Typically, this achieved by immunohistological analyses, sorting, or single-cell RNA-sequencing. Alternatively, cell-specific DNA methylome information can be leveraged to deconvolute fractions from a bulk mixture. However, comprehensive benchmarking deconvolution methods modalities was not yet performed. Here...