A5052526983- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Advanced Chemical Sensor Technologies
- Colorectal Cancer Screening and Detection
- Pancreatic and Hepatic Oncology Research
- Radioactive contamination and transfer
- Blood transfusion and management
- Nuclear Physics and Applications
- Epigenetics and DNA Methylation
- Isotope Analysis in Ecology
- Medical Imaging and Pathology Studies
- Biochemical and Molecular Research
- SARS-CoV-2 detection and testing
- Colorectal Cancer Treatments and Studies
- Cervical Cancer and HPV Research
- Biosensors and Analytical Detection
- Vascular Procedures and Complications
- X-ray Spectroscopy and Fluorescence Analysis
- Advanced Proteomics Techniques and Applications
- Infectious Aortic and Vascular Conditions
- Colorectal Cancer Surgical Treatments
- Genetic factors in colorectal cancer
- Endometrial and Cervical Cancer Treatments
- MicroRNA in disease regulation
Imperial College London
2018-2024
St Mary's Hospital
2021
St. Mary's Hospital
2021
St. Mary’s Hospital
2020
St Mary's Hospital
2019
The London College
2018
Laiko General Hospital of Athens
2005
Clearance of surgical margins in cervical cancer prevents the need for adjuvant chemoradiation and allows fertility preservation. In this study, we determined capacity rapid evaporative ionization mass spectrometry (REIMS), also known as intelligent knife (iKnife), to discriminate between healthy, preinvasive, invasive tissue. Cervical tissue samples were collected from women with human papilloma virus (HPV) ± intraepithelial neoplasia (CIN), or cancer. A handheld diathermy device generated...
The genomic landscape of colorectal cancer (CRC) is shaped by inactivating mutations in tumour suppressors such as APC, and oncogenic mutant KRAS. Here we used genetically engineered mouse models, multimodal mass spectrometry-based metabolomics to study the impact common genetic drivers CRC on metabolic intestine. We show that untargeted profiling can be applied stratify intestinal tissues according underlying alterations, use spectrometry imaging identify tumour, stromal normal adjacent...
In this study, we integrate rapid evaporative ionization mass spectrometry (REIMS) with the Harmonic scalpel, an advanced laparoscopic surgical instrument that utilizes ultrasound energy to dissect and coagulate tissues. It provides unparalleled manipulation capability surgeons has superseded traditional electrosurgical tools particularly in abdominal surgery, but is yet be validated REIMS. The REIMS platform coupled device was shown produce tissue-specific lipid profiles through analysis of...
Abstract Instantaneous, continuous, and reliable information on the molecular biology of surgical target tissue could significantly contribute to precision, safety, speed intervention. In this work, we introduced a methodology for chemical identification in robotic surgery using rapid evaporative ionisation mass spectrometry. We developed aerosol evacuation system that is compatible with platform enabling consistent intraoperative sample collection assessed feasibility during head neck...
Introduction: Delays in the diagnosis and treatment of endometrial cancer negatively impact patient survival. The aim this study was to establish whether rapid evaporative ionisation mass spectrometry using iKnife can accurately distinguish between normal malignant biopsy tissue samples real time, enabling point-of-care (POC) diagnoses. Methods: Pipelle were obtained from consecutive women needing biopsies for clinical reasons. A Waters G2-XS Xevo Q-Tof spectrometer used conjunction with a...
Transanal minimally invasive surgery (TAMIS) is deployed for organ preservation in early rectal cancer and significant polyps. Rapid evaporative ionisation mass spectrometry (REIMS) provides biochemical tissue analysis, which could be applied intraoperatively to give real-time feedback the surgeon decrease risk of an involved margin. However, accuracy feasibility this approach have not been established.In prospective observational study, patients undergoing resection adenomas or carcinomas...
Objective: Rapid evaporative ionization mass spectrometry (REIMS) is a metabolomic technique analyzing tissue metabolites, which can be applied intraoperatively in real-time. The objective of this study was to profile the lipid composition colorectal tissues using REIMS, assessing its accuracy for real-time recognition and risk-stratification. Summary Background Data: Metabolic dysregulation hallmark feature carcinogenesis; however, it remains unknown if leveraged clinical applications...
Ambient Ionisation Mass Spectrometry techniques: Desorption Electrospray (DESI) and Laser – Rapid Evaporative (LD-REIMS) were used to detect the SARS-CoV-2 in dry nasal swabs. 45 patients studied from samples collected between April June 2020 a clinical feasibility study. Diagnostic accuracy was calculated as 86.7% 84% for DESI LD-REIMS respectively. Results can be acquired seconds providing robust quick analysis of COVID-19 status which could carried out without need centralised laboratory....
Rapid evaporative ionization mass spectrometry (REIMS) is a direct tissue metabolic profiling technique used to accurately classify tissues using pre-built spectral databases. The reproducibility of the analytical equipment, methodology and classification algorithms has yet be evaluated over multiple sites, which an essential step for developing this future clinical applications. In study, we harmonized REIMS single-source reference material across four sites with identical equipment:...
Abstract Introduction The aim of this study was to determine the diagnostic accuracy REIMS for dysplasia in human adenomas and early colorectal cancer changes mucosal lipid chemistry during initiation. Moreover, we attempted develop a proof concept first man novel REIMS-based endoscope in-vivo chemical phenotyping based on real time analysis phosphatidic acid (PA) phosphatidylethanolamine (PE) metabolism. Experimental procedure A prospective, observational cross-sectional cohort performed...