A5090267257- Angiogenesis and VEGF in Cancer
- Connexins and lens biology
- Retinal Development and Disorders
- Galectins and Cancer Biology
- Protein Tyrosine Phosphatases
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Ocular Oncology and Treatments
- Glaucoma and retinal disorders
- Retinal and Macular Surgery
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Retinal Diseases and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Receptor Mechanisms and Signaling
- Cancer Cells and Metastasis
- Toxic Organic Pollutants Impact
- Neuropeptides and Animal Physiology
- Estrogen and related hormone effects
- Renin-Angiotensin System Studies
- Chronic Kidney Disease and Diabetes
- Ocular Disorders and Treatments
- Air Quality and Health Impacts
- Phytoestrogen effects and research
- Pharmacological Receptor Mechanisms and Effects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Harvard University
2017-2025
Smith-Kettlewell Eye Research Institute
2017-2025
Massachusetts Eye and Ear Infirmary
2017-2025
Binzhou University
2015-2024
Binzhou Medical University
2015-2024
Central South University
2018-2019
Second Xiangya Hospital of Central South University
2018-2019
China Pharmaceutical University
2018
The University of Texas Health Science Center at San Antonio
2015-2017
Abstract The endothelial glycocalyx, located at the luminal surface of endothelium, plays an important role in regulation leukocyte adhesion, vascular permeability, and homeostasis. Endomucin (EMCN), a component is mucin-like transmembrane glycoprotein selectively expressed by venous capillary endothelium. We have previously shown that knockdown EMCN impairs retinal development vivo growth factor 165 isoform (VEGF165)-induced cell migration, proliferation, tube formation human cells vitro...
The increased PD-L1 expression induces poorer prognosis in melanoma. small molecule inhibitors of PD-1/PD-L1 pathways have been an encouraging drug development strategy because good affinity and oral bioavailability without immunogenicity immunotoxicities antibodies. In this study, we studied the effects PCC0208025 (BMS202), a inhibitor PD-L1, on binding cytokines secretion human CD3+ cells vitro. We also investigated antitumor immunomodulatory activity pharmacokinetics properties B16-F10...
We have previously shown that knockdown of endomucin (EMCN), an integral membrane glycocalyx glycoprotein, prevents VEGF-induced proliferation, migration, and tube formation in vitro angiogenesis vivo. In the endothelium, VEGF mediates most its angiogenic effects through receptor 2 (VEGFR2). To understand role EMCN, we examined effect EMCN depletion on VEGFR2 endocytosis activation. Results showed although stimulation promoted internalization control endothelial cells (ECs), loss prevented...
Connexins play essential roles in lens homeostasis and development. Here, we identified a new role for Cx50 that mediates cell-cell adhesion function. enhanced the adhesive capability of AQP0. Interestingly, expression alone promoted cell at comparable level to AQP0; however, this function was not observed with other connexins, Cx43 Cx46. Moreover, property occurred both homotypic expressed pairing cells heterotypic only one cell, appears be unrelated its forming gap junction channels. KO...
Tris-(2,3-dibromopropyl) isocyanurate (TDBP-TAZTO), an emerging brominated flame retardant, possesses the characteristics of candidate persistent organic pollutants and has displayed toxicity to fish rodents. TDBP-TAZTO can pass through blood brain barrier accumulate in brain. However, neurotoxicity not yet studied We hypothesize that could induce rat hippocampal neurons. The male adult rats were exposed 5 50 mg/kg by gavage, daily for 6 months. resulted cognitive impairment depression-like...
The increased PD-L1 induces poorer prognosis in melanoma. treatment with PD-1/PD-L1 antibodies have a low response rate. combination immunotherapies are the encouraging drug development strategy to receive maximal therapeutic benefit. In this study, we investigated enhanced antitumor and immunomodulatory activity of combined SEP αPD-L1 B16-F10 melanoma-bearing mice. results shown that presented significant synergistic effects, frequency CD8+ CD4+ T cells spleen tumor, cytotoxic CTL spleen,...
To investigate the long-term outcomes and complications of scleral-fixated intraocular lens (SFIOL) implantation without conjunctival peritomies sclerotomy in patients with a history ocular trauma inadequate capsular support during primary pars plana vitrectomy or silicone oil removal.Records who underwent SFIOL removal.Sixty-nine eyes 69 were included this study. The median follow-up period was 34 months (range, 6-99 months). average patient age at time surgery 44 years old 4-80 years). At...
Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of endothelial cell glycocalyx. We have previously shown that EMCN necessary for vascular growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling. To explore structural components are its function molecular mechanism in VEGF-induced functions, we generated a series mouse truncation mutants examined their ability to rescue functions human primary cells (EC) which endogenous...
Tris-(2,3-dibromopropyl) isocyanurate (TDBP-TAZTO), an emerging brominated flame retardant, possesses the characteristics of candidate persistent organic pollutants and has displayed toxicity to fish rodents. TDBP-TAZTO can pass through blood-brain barrier accumulate in brain. might also induce neuronal cell toxicity. However, neurotoxicity mechanisms have not yet been studied. We hypothesize that could mouse hippocampal neurons SH-SY5Y cells. The mice were exposed 5 50 mg/kg by gavage,...
The murine double minute (MDM)2 is a critical negative regulator of the p53 tumor suppressor, and MDM2 SNP309G associated with higher risk proliferative vitreoretinopathy (PVR); in addition, T309G created using clustered regularly interspaced short palindromic repeats (CRISPR)/associated endonuclease (Cas)9 enhances normal rabbit vitreous-induced expression survival primary human retinal pigment epithelial (hRPE) cells vitro. goal this study was to determine whether contributes development...
Galectin-3 (Gal3) is a carbohydrate-binding protein reported to promote angiogenesis by influencing vascular endothelial growth factor-A receptor 2 (VEGFR2) signal transduction. Here we evaluated whether the ability of Gal3 function as an angiogenic factor involved (VEGF). To address this possibility used human retinal microvascular cells (HRECs) determine exogenous requires VEGF activate VEGFR2 signaling and if required for VEGFR2. phosphorylation HREC migration assays, following either...
Endomucin (EMCN), an endothelial-specific glycocalyx component, was found to be highly expressed by the endothelium of renal glomerulus. We reported anti-inflammatory role EMCN and its involvement in regulation vascular endothelial growth factor (VEGF) activity through modulating VEGF receptor 2 (VEGFR2) endocytosis. The goal this study is investigate phenotypic functional effects deficiency using first global knockout mouse model.