The present experiment was conducted to investigate the effect of heat stress (HS) andstocking density (SD) on growth performance, breast meat quality, and intestinal barrier functionin broiler chickens. Experimental treatments included two different ambient temperatures (20 °C:thermoneutral conditions, or 27.8 °C: HS conditions) SD (low: 9 birds/m2 andhigh: 18 birds/m2) in a 2 × factorial arrangement. A total 1140 21-day-old chickens wereallotted 1 4 with five replicates. At end (35 days...

The objective of the current experiment was to investigate effect stocking density and sex on growth performance, meat quality, intestinal barrier function in broiler chickens. conducted a completely randomized design with 2 × 4 factorial arrangement consisting four different densities battery cages. A total 540 1-d-old Ross 308 chickens were allotted one eight treatments five replicates. Within each sex, birds raised at 15.2, 20.2, 25.3, or 30.4 birds/m2 from 1 28 d age. Different achieved...

The objective of this study was to investigate the effects dietary Bacillus subtilis supplementation on growth performance, jejunal lesion scores, oocyst shedding, and cytokine tight junction protein expression in broiler chickens infected with Eimeria maxima. A total 196 male day-old Ross 708 broilers were given a nonexperimental diet until 14 D age. Then, all randomly assigned one seven treatments: 2 basal diets (CON NC); CON + virginiamycin (AB1); bacitracin methylene disalicylate (BMD;...

The study was conducted to investigate the effects of different Eimeria inoculation doses on growth performance, gut ecosystem, and body composition broilers in floor pens for 35 days. A total 750 15-day-old were allocated five experimental groups with six replicate pens. included unchallenged control (CON); dose 1 (ED1): E. acervulina: 31,250/E. maxima: 6250/E. tenella: 6250; 2 (ED2): 62,500/E. 12,500/E. 12,500; 3 (ED3): 125,000/E. 25,000/E. 25,000; 4 (ED4): 250,000/E. 50,000/E. 50,000. On...

The objective of this study was to investigate the effects different doses Eimeria maxima (EM) oocysts on growth performance and intestinal health in broiler chickens challenged with a dual infection model necrotic enteritis (NE) using EM NetB+Clostridium perfringens (CP). A total 432 fourteen-d-old male Cobb 500 were divided into 6 groups replicates each. six as follows: Control, non-challenged; T0+, CP at 1 × 109 colony forming unit; T5K+, T0+ + 5,000 oocysts; T10K+, 10,000 T20K+; 20,000...

Chickens have undergone genetic improvements in the past few decades to maximize growth efficiency. However, necrotic enteritis (NE), an enteric disease primarily caused by C. perfringens, remains a significant problem poultry production. A study investigated differences intestinal health between non-selected meat-type Athens Canadian Random Bred (ACRB) and modern Cobb 500 (Cobb) broilers when challenged with experimental NE. The utilized 2 × 3 factorial arrangement, consisting of two main...

This study investigated transcriptomic differences between the caudal and cranial areas in breast muscle grower phase of broiler chickens (D 21 D 28). A total 66 one-day-old were allotted to 6 cage pens 11 birds per pen. On 28, one bird pen was randomly selected, samples collected Pectoralis major. RNA sequencing conducted, followed by screening for differentially expressed genes (DEGs; P < 0.05) multivariate analyses. 24,498 identified, 8,831 had greater than 100 mean read count. 21, there...

An experiment was conducted to investigate the changes in gastrointestinal physiology, including intestinal leakage, immune response, oxidative stress, along with performance traits, of Hy-Line W-36 laying hens following Eimeria infection at peak egg production. A total 360 hens, 25 wk age, were assigned randomly 5 treatment groups, each consisting 6 replicate cages, a nonchallenged control group. The other 4 groups inoculated graded levels mixed species as high, medium-high, medium-low, and...

Two studies were conducted to evaluate the effects of maltol as a postbiotic on innate immunity, gut health, and enteric infection. In first study, an in vitro culture system was used immune response chicken macrophage cells (CMC), integrity intestinal epithelial (IEC), anti-parasitic activity against Eimeria maxima , differentiation quail muscle (QMC) primary embryonic (PMC). All seeded 24-well plates treated with at concentrations 0.1, 1.0, 10.0 μg. CMC IEC stimulated by lipopolysaccharide...

Mouse monoclonal antibodies (mAbs) reactive with Clostridium perfringens collagen adhesin protein (CNA) and necrotic enteritis B-like toxin (NetB) were developed. The best capture/detection mAb pairs for CNA NetB selected based on their affinity specificity to develop sandwich enzyme-linked immunosorbent assays (ELISAs) detect proteins, respectively, in jejunal digesta samples from commercial broiler farms the United States. Prior analysis of flocks, sensitivity ELISAs validated using sera,...

Necrotic enteritis (NE) is a widespread infectious disease caused by Clostridium perfringens that inflicts major economic losses on the global poultry industry. Due to regulations antibiotic use in production, there an urgent need for alternative strategies mitigate negative effects of NE. This paper presents passive immunization technology utilizes hyperimmune egg yolk immunoglobulin Y (IgY) specific immunodominant antigens C. perfringens. Egg IgYs were generated immunizing hens with four...

Water-miscible vitamin E (WVE) has been known to be more bioavailable than conventional lipid-soluble (LVE) in human foods because of its greater solubility the gastrointestinal tract. However, no data regarding comparison efficacy WVE LVE were available poultry diets. The objective current experiment was investigate effect (VE) sources and inclusion levels diets on growth performance, meat quality, alpha-tocopherol retention, intestinal inflammatory cytokine expression broiler chickens. A...

Vitamin D

Two in vivo and vitro studies were conducted to investigate the effects of supplementation a natural polyherbal mixture (PHM) manufactured from selected herbs broiler chickens challenged with coccidiosis. For trial, E. tenella maxima sporozoites used test how PHM affected viability at 24, 48, 72 h. The treatments as follows: negative control phosphate buffered saline (NC-PBS), solvent containing 1 % dimethyl sulfoxide (DMSO), salinomycin 12 mg/kg DMSO PBS (SAL), 500 (PHM). total 288...

A study was conducted to investigate the effects of tannic acid (TA) supplementation on growth performance, gut health, antioxidant capacity, microbiota, and meat yield quality in broilers raised for 42 days. total 700 one-day-old male broiler chickens (Cobb500) were allocated into 5 treatments with 7 replicates 20 birds per pen. There five treatments: 1) 0 (TA0: basal diet without TA); 2) 0.25 (TA0.25: diet+0.25 g/kg 3) 0.5 (TA0.5: diet+0.5 4) 1 (TA1: diet+1 5) 2 (TA2: diet+2 TA). The...

Mycotoxins, particularly deoxynivalenol (DON), are common contaminants in feed ingredients such as corn distiller's dried grains with solubles (DDGS) and pose significant risks to poultry health. This study investigated the effects of feeding naturally DON contaminated DDGS on growth performance, body composition, immunological response, gastrointestinal health young pullets. A total 360, 4-week-old Hy-Line W36 pullets were randomly assigned diets increasing levels (0, 5, 10, 15, 20%) over...

Two studies were conducted to evaluate the effects of indole-3-carboxylate (ICOOH) as a postbiotic on maintaining intestinal homeostasis against avian coccidiosis. In first study, an in vitro culture system was used investigate ICOOH proinflammatory cytokine response chicken macrophage cells (CMCs), gut integrity epithelial (IECs), differentiation quail muscle (QMCs), and primary embryonic (PMCs) anti-parasitic effect Eimeria maxima . Cells be tested seeded 24-well plates treated with at...

Two experiments were conducted to evaluate the effects of dietary sophorolipids (SLs) supplementation as antibiotic alternatives on growth performance and gut health chickens infected with Eimeria maxima. In experiment 1, 336 (zero-day-old) male broilers used. The weighed randomly allocated following 6 treatments groups 7 chickens/cage 8 cages/treatment: control group that received a basal diet (NC), positive was challenged E. maxima (PC), PC+C18:1 lactonic diacetyled SL (SL1), deacetyled...