A5100662622- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Associations and Epidemiology
- Cancer-related gene regulation
- Congenital heart defects research
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Multiple Myeloma Research and Treatments
- Pluripotent Stem Cells Research
- Bioinformatics and Genomic Networks
- Genomic variations and chromosomal abnormalities
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Immune cells in cancer
- Genetics and Neurodevelopmental Disorders
- Hemoglobinopathies and Related Disorders
- Obesity, Physical Activity, Diet
- Statistical Methods in Clinical Trials
- Genetic and phenotypic traits in livestock
- Global Cancer Incidence and Screening
- Traumatic Brain Injury and Neurovascular Disturbances
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Genetic diversity and population structure
Augusta University
2016-2025
Dalian Medical University
2024
Chongqing Medical University
2024
Second Affiliated Hospital of Chongqing Medical University
2024
The First People's Hospital of Xiaoshan District, Hangzhou
2024
Xinxiang Medical University
2024
Center for Tuberculosis Control of Guangdong Province
2024
Augusta University Health
2012-2023
University of California, San Diego
2017-2023
Shanxi Medical University
2021
Mutations in lysosomal-associated membrane protein 2 (LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome-lysosome fusion human cardiomyocytes (CMs). Remarkably, LAMP-2B functions independently of syntaxin 17 (STX17), a that is essential non-CMs. Instead, interacts autophagy related 14 (ATG14) and vesicle-associated 8 (VAMP8) its...
DNA methylation, mediated by the combined action of three methyltransferases (DNMT1, DNMT3A, and DNMT3B), is essential for mammalian development a major contributor to cellular transformation. To elucidate how methylation targeted, we mapped genome-wide localization all DNMTs examined relationships among these markers, histone modifications, nucleosome structure in pluripotent human tumor cell line its undifferentiated differentiated states. Our findings reveal strong link between...
Abstract Ovarian injury because of chemotherapy can decrease the levels sexual hormones and potentia generandi patients, thereby greatly reducing quality life. The goal this study was to investigate which transplantation method for human umbilical cord mesenchymal stem cells ( HUMSC s) recover ovarian function that has been damaged by chemotherapy. A rat model established using an intraperitoneal injection cyclophosphamide. Membrane‐labelled s were subsequently injected directly into ovary...
The presence of polyfunctional CD4+ T cells is often associated with favorable antitumor immunity. We report here that persistent activation signal transducer and activator transcription 5 (STAT5) in tumor-specific drives the development cells. showed ectopic expression a constitutively active form murine STAT5A (CASTAT5) enabled to undergo robust expansion, infiltrate tumors vigorously, elicit CD8+ cell responses adoptive transfer model system. Integrated epigenomic transcriptomic analysis...
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a powerful platform for biomedical research. However, they immature, which is barrier to modeling adult-onset cardiovascular disease. Here, we sought develop simple method that could drive cultured hiPSC-CMs toward maturity across number of phenotypes, with the aim utilizing mature model human were in fatty acid-based medium and plated on micropatterned surfaces. These cells display many characteristics adult...
Quantification of inflammation in tissue samples can be a time-intensive bottleneck therapeutic discovery and preclinical endeavors. We describe versatile rapid approach to quantitatively assay macrophage burden intact samples. Perfluorocarbon (PFC) emulsion is injected intravenously, the droplets are effectively taken up by monocytes macrophages. These 'in situ' labeled cells participate inflammatory events vivo resulting PFC accumulation at loci. Necropsied tissues or organs subjected...
Anaplastic thyroid carcinoma (ATC) accounts for only 3% of cancers, yet strikingly, it almost 40% cancer deaths. Currently, no effective therapies exist. In an effort to identify ATC-specific therapeutic targets, we analyzed global gene expression data from multiple studies dysregulated genes.The National Center Biotechnology Information Gene Expression Omnibus database was searched high-throughput microarray human ATC tissue along with normal and/or papillary (PTC) tissue. levels in were...
In recent years, the immune-potentiating effects of some widely used chemotherapeutic agents have been increasingly appreciated. This provides a rationale for combining conventional chemotherapy with immunotherapy strategies to achieve durable therapeutic benefits. Previous studies implicated immunomodulatory melphalan, an alkylating agent commonly treat multiple myeloma, but underlying mechanisms remain obscure. present study, we investigated impact melphalan on endogenous immune cells as...
Fluorine-19 MRI is an emerging cellular imaging approach, enabling lucid, quantitative "hot-spot" with no background signal. The utility of 19F-MRI to detect inflammation and cell therapy products in vivo could be expanded by improving the intrinsic sensitivity probe molecular design. We describe a metal chelate based on salicylidene-tris(aminomethyl)ethane core, solubility perfluorocarbon (PFC) oils, potent accelerator 19F longitudinal relaxation time (T1). Shortening T1 can increase image...
Congenital heart defects (CHDs) are frequent in children with Down syndrome (DS), caused by trisomy of chromosome 21. However, the underlying mechanisms poorly understood. Here, using a human-induced pluripotent stem cell (iPSC)-based model and Dp(16)1Yey/+ (Dp16) mouse DS, we identified downregulation canonical Wnt signaling downstream increased dosage interferon (IFN) receptors (IFNRs) genes on 21 as causative factor cardiogenic dysregulation DS. We differentiated human iPSCs derived from...
Abstract Background Leukemias driven by activated, chimeric FGFR1 kinases typically progress to AML which have poor prognosis. Mouse models of this syndrome allow detailed analysis cellular and molecular changes occurring during leukemogenesis. We used these determine the effects leukemia development on immune cell composition in microenvironment progression. Methods Single RNA sequencing (scRNA-Seq) was characterize associated neutrophils define gene expression cells Results scRNA-Seq...
Abnormalities in Ca2+ homeostasis are associated with cardiac arrhythmias and heart failure. Triadin plays an important role cardiomyocytes. Alternative splicing of a single triadin gene produces multiple isoforms. The cardiac-predominant isoform, mouse MT-1 or human Trisk32, is encoded by exons 1 to 8. In humans, mutations the that lead reduction Trisk32 levels can cause dysfunction arrhythmias. Decreased also common patients However, mechanisms maintain isoform composition remain...
To evaluate the role of infiltrating macrophages in murine models single and double mutation head neck tumors using a novel fluorine-19 (19 F) MRI technology.Tumor cell lines single-hit/SCC4 or double-hit/Cal27, with mutations TP53 & FHIT, respectively, were injected bilaterally into flanks (n = 10) female mice. With established, perfluorocarbon nanoemulsion was intravenously, which labels situ predominantly monocytes macrophages. Longitudinal spin density-weighted 19 F data enabled...
Indoleamine 2,3 dioxygenase-1 (IDO-1) is an enzyme in the kynurenine pathway which augments tumor-induced immune tolerance. Previous studies childhood acute myeloid leukemia (AML) have shown a negative correlation of IDO-1 mRNA expression with outcomes. The aim our study was to develop practical and objective immunohistochemical technique quantify on diagnostic bone marrow biopsies AML patients order facilitate its use routine clinical practice. extracted from specimens 29 patients. protein...
The aim of the present study was to determine best anthropometric discriminators type 2 diabetes mellitus (T2DM) among White and Black males females in a large US sample.We used Atherosclerosis Risk Communities baseline data (1987-89) from 15 242 participants (1827 with T2DM) aged 45-65 years. Anthropometric measures included body shape index (ABSI), adiposity (BAI), mass index, waist circumference (WC), waist: height ratio (WHtR), hip (WHR). All were standardized Z-scores. Using logistic...
To determine which anthropometric measures are the strongest discriminators of incident type 2 diabetes (T2DM) among White and Black males females in a large U.S. cohort.We used Atherosclerosis Risk Communities study data from 12,121 participants aged 45-64 years without at baseline who were followed for over 11 years. Anthropometric included body shape index (ABSI), adiposity (BAI), mass (BMI), waist circumference (WC), to hip ratio (WHR), height (WHtR), (WHHR). All repeated each visit...
The COVID-19 pandemic caused by the novel SARS-CoV-2 coronavirus has drastically altered global realities. Harnessing national scale data from may better inform policy makers in decision making surrounding reopening of society. We examined country-level, daily-confirmed, case World Health Organization (WHO) to understand comparative dynamics associated with ongoing at a scale.Observational study.We included 20 countries Europe, Americas, Africa, Eastern Mediterranean and West Pacific...
Due to their high-quality characteristics, Chinese hamster ovary (CHO) cells have become the most widely used and reliable host for production of recombinant therapeutic proteins in biomedical field. Previous studies shown that m6A reader YTHDF3, which contains YTH domain, can affect a variety biological processes by regulating translation stability target mRNAs. This study investigates effect YTHDF3 on transgenic CHO cells. The results indicate stable overexpression significantly enhances...
Objective: Clinical experience and patient-level physiological studies indicate that albuterol transiently reduces oxygen saturation, irrespective of the underlying respiratory condition causing wheezing. Three revisions Cochrane review, Bronchodilators for Bronchiolitis (Review), have found temporarily increases saturation. Rarely, these findings were even statistically significant, but direction effect is consistently opposite from studies. In this study, we attempted to resolve apparent...
Abstract Summary: The ‘omic’ data such as genomic data, transcriptomic proteomic and single nucleotide polymorphism have been rapidly growing. omic are large-scale high-throughput data. Such challenge traditional statistical methodologies require multiple tests. Several multiple-testing procedures Bonferroni procedure, Benjamini–Hochberg (BH) procedure Westfall–Young developed, among which some control family-wise error rate the others false discovery (FDR). These valid in cases cannot be...