A5044025252- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Biomedical Research and Pathophysiology
- Renal Diseases and Glomerulopathies
- SARS-CoV-2 and COVID-19 Research
- Cancer Immunotherapy and Biomarkers
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Bipolar Disorder and Treatment
- Asthma and respiratory diseases
- Advanced biosensing and bioanalysis techniques
- Mast cells and histamine
- Virus-based gene therapy research
CureVac (Germany)
2014-2024
Center for Integrated Protein Science Munich
2021
Ludwig-Maximilians-Universität München
2021
University of Göttingen
2009-2020
Universitätsmedizin Göttingen
2020
Paul Ehrlich Institut
2016
Friedrich-Alexander-Universität Erlangen-Nürnberg
2006-2011
Abstract mRNA represents a promising new vaccine technology platform with high flexibility in regard to development and production. Here, we demonstrate that vaccines based on sequence optimized, chemically unmodified formulated optimized lipid nanoparticles (LNPs) are highly immunogenic well tolerated non-human primates (NHPs). Single intramuscular vaccination of NHPs LNP-formulated mRNAs encoding rabies or influenza antigens induced protective antibody titers, which could be boosted...
Protein‐ and peptide‐based tumor vaccines depend on strong adjuvants to induce potent immune responses. Here, we demonstrated that a recently developed novel adjuvant based non‐coding, long‐chain RNA molecule, termed RNAdjuvant ® , profoundly increased immunogenicity of both antigen formats. induced balanced, long‐lasting responses resulted in anti‐tumor activity. A direct comparison Poly(I:C) showed superior efficacy our enhance antigen‐specific multifunctional CD8 + T‐cell mediate by...
Abstract The vigorous response of IgG-switched memory B cells to recurring pathogens involves enhanced signalling from their B-cell antigen receptors (BCRs). However, the molecular signal amplification mechanisms memory-type BCRs remained unclear. Here, we identify immunoglobulin tail tyrosine (ITT) motif in cytoplasmic segments membrane-bound IgGs (mIgGs) as principle device higher vertebrates and decipher its microanatomy. We show that different families protein kinases act upstream...
The random nature of T-cell receptor-β (TCR-β) recombination needed to generate immunological diversity dictates that two-thirds alleles will be out-of-frame. Transcripts derived from nonproductive rearrangements are cleared by the nonsense-mediated mRNA decay (NMD) pathway, process which cells selectively degrade transcripts harboring premature termination codons. Here, we demonstrate fetal thymus in transgenic mice ubiquitously express a dominant-negative form Rent1/hUpf1, an essential...
Abstract Secondary antibody responses are marked by faster kinetics, improved affinity and a switch from IgM to other immunoglobulin isotypes, most notably IgG, compared with primary responses. These changes protect reinfection represent the principle of vaccination strategies. Yet, molecular mechanisms that underlie B-cell memory unclear. Here we show, inactivating tail tyrosine (ITT) signalling motif membrane-bound IgG1 in mouse, ITT facilitates maintenance reactivation IgG-switched B...
B-lymphocyte development is dictated by the protein products of functionally rearranged Ig heavy (H) and light (L) chain genes. rearrangement begins in pro-B cells at IgH locus. If generate a productive allele, they assemble pre-B cell receptor complex, which signals their differentiation into clonal expansion. Pre-B are also thought to contribute allelic exclusion preventing further rearrangements. Here we show two independent mouse models that accumulation stabilized μH mRNA does not...
Regulation throughout B cell maturation and activation prevents autoreactive cells from entering germinal center (GC) reactions. This study shows that a subset of in V(H)3H9 micro IgH transgenic mice escapes these serial checkpoints proceeds into splenic GC. GC isolated all express the heavy chain, some co-express light chains yield an anti-dsDNA specificity have somatic mutations, consistent with their origin. Nonetheless, tolerance is ultimately preserved as serum titers antibodies are not...
Abstract Inaccurate VDJ rearrangements generate a large number of progenitor (pro)-B cells with two nonproductive IgH alleles. Such lack essential survival signals mediated by surface IgM heavy chain (μH chain) expression and are normally eliminated. However, secondary upstream VH gene segments into assembled exons have been described in mice transgenic for productive μH chains, process known as replacement. If replacement was independent signals, it could also modify thus rescue pro-B...
Immunotherapy has revolutionized cancer treatment in recent years. Although currently approved checkpoint inhibitors (CPIs) yield remarkable anti-tumoral responses several types, a substantial proportion of patients do not benefit from such therapies. Local activation innate immune signaling pathways is promising approach to overcome the immunosuppressive tumor microenvironment, induce anti-tumor immunity, and improve efficacy CPI Here, we assessed mode action RNA-based stimulator CV8102 for...
Meeting abstracts Purified recombinant proteins and peptides, which are currently under development in various anti-cancer vaccination approaches, lack sufficient immunogenicity. Therefore, potent adjuvants needed to induce strong persistent anti-tumor immunity. However, only few
<h3>Background</h3> Adoptive T cell therapy (ACT) has demonstrated remarkable efficacy in treating hematological malignancies. However, its clinical benefit solid tumors remains limited and short-lasting. Despite supplying large numbers of cells for initial tumor reduction, ACT faces challenges such as inadequate penetration, functionality durability, well the immunosuppressive microenvironment. To address these limitations, vaccination is being explored trials a strategy to enhance...
Abstract Immunoglobulin β (Ig‐β) is a critical signal transducer of precursor B cell and receptors. B29, the gene coding for Ig‐β, switched on in progenitor cells expressed until terminal stage antibody‐producing plasma cells. Although several cis ‐acting elements transcription factors required B29 expression have been characterized lines, vivo significance individual motifs located 1.2‐kb promoter region remained unclear. To address whether this drives lineage‐specific mice as efficiently...
Abstract Purified recombinant proteins and peptides, which are currently under development in various anti-cancer vaccination approaches, lack sufficient immunogenicity. Therefore, potent adjuvants needed to induce strong persistent anti-tumor immunity. However, only few licensed, most of primarily enhance antibody, but not T cell responses. Here, we demonstrate that a novel, well defined, thoroughly characterized RNA-based adjuvant mediates balanced long-lasting humoral cellular immune Our...