A5030384982- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Phytochemistry and Bioactivity Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Cholinesterase and Neurodegenerative Diseases
- Retinoids in leukemia and cellular processes
- Plant Virus Research Studies
- Enzyme function and inhibition
- Metal-Catalyzed Oxygenation Mechanisms
- Cancer-related gene regulation
- Toxin Mechanisms and Immunotoxins
- Pluripotent Stem Cells Research
- Plant-based Medicinal Research
- Coenzyme Q10 studies and effects
- Pharmacological Effects of Natural Compounds
- Virus-based gene therapy research
- Neurogenetic and Muscular Disorders Research
- Bacteriophages and microbial interactions
- Amyotrophic Lateral Sclerosis Research
- Redox biology and oxidative stress
- interferon and immune responses
- Genomics and Chromatin Dynamics
- Immune cells in cancer
Case Western Reserve University
2016-2021
University School
2018
Chimeric antigen receptor T cells (CAR-T cell) targeting CD19 are effective against several subtypes of CD19-expressing hematologic malignancies. Centralized manufacturing has allowed rapid expansion this cellular therapy, but it may be associated with treatment delays due to the required logistics. We hypothesized that point care CAR-T on automated CliniMACS Prodigy® device allows reproducible and fast delivery for patients non-Hodgkin’s lymphoma. Here we describe cell results characterize...
Mitochondrial dysfunction is a major cytopathology in Huntington's disease (HD), fatal and inherited neurodegenerative disease. However, the molecular mechanisms by which disease-causing gene, mutant Huntingtin (mtHtt), affects mitochondrial function remains elusive. This study aims to determine role that Mitogen-activated protein kinase 1 (MAPK1) plays over-activation of Dynamin-related (Drp1), fission protein, leads neurodegeneration seen HD. We show MAPK1 binds phosphorylates Drp1 vitro....
SUMMARY Human embryonic stem cell (hESC)-derived neurons have the potential to model neurodegenerative disorders. Here, we demonstrate expression of a mutant gene, superoxide dismutase 1(SOD1), linked familial amyotrophic lateral sclerosis (ALS) in hESC-derived motor neurons. Green fluorescent protein (GFP) under control HB9 enhancer was used identify SOD1-transfected that express human wild-type SOD1 or one three different mutants (G93A, A4V and I113T) SOD1. Neurons transfected with...
Plant vital nanoparticle PVX shows a binding affinity towards malignant B cells and traffics to metastatic lymphoma sites in mice challenged with cell lymphoma, thus enabling NHL-specific drug delivery.
Abstract Acute myeloid leukemia (AML) is characterized by a differentiation block in progenitors resulting aberrant proliferation of immature cells. Therapeutics that induce leukemic cells would overcome the high toxicity and low potency conventional chemotherapy. Securinine, plant-derived alkaloid from Securinega suffruticosa, has been shown to be promising AML agent through an unclear mechanism, but limited its (~20μM) toxicity. We performed lead optimization chemistry on securinine led...
Human embryonic stem cell (hESC)-derived neurons have the potential to model neurodegenerative disorders.Here, we demonstrate expression of a mutant gene, superoxide dismutase 1 (SOD1), linked familial amyotrophic lateral sclerosis (ALS) in hESC-derived motor neurons.Green fluorescent protein (GFP) under control HB9 enhancer was used identify SOD1-transfected that express human wildtype SOD1 or one three different mutants (G93A, A4V and I113T) SOD1.Neurons transfected with exhibited reduced...
Acute Myeloid Leukemia (AML) is a rapidly developing cancer of the bone marrow, with five‐year survival rate 27.4%. Current methods treatment are toxic to older patients, creating need for new options that more optimal patient's health. One potential securinine, natural compound from Securinega suffruticosa plant inhibits AML cell growth. Novel securinine derivatives were tested through viability assays, and 250 was found exhibit anti‐cancer properties at higher potency lower toxicity than...