A5079278683- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Immune Cell Function and Interaction
- interferon and immune responses
- Aquatic life and conservation
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Identification and Quantification in Food
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- melanin and skin pigmentation
- Viral Infections and Vectors
- Barrier Structure and Function Studies
- Dermatology and Skin Diseases
- Insect Utilization and Effects
- Marine animal studies overview
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- Vector-Borne Animal Diseases
- Dietary Effects on Health
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Pharmacological Effects of Natural Compounds
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
Institute of Hydrobiology
2021-2025
Chinese Academy of Sciences
2021-2025
Huazhong Agricultural University
2017-2022
Temple University
2014
The olfactory organ of vertebrates receives chemical cues present in the air or water and, at same time, they are exposed to invading pathogens. Nasal-associated lymphoid tissue (NALT), which serves as a mucosal inductive site for humoral immune responses against antigen stimulation mammals, is also teleosts. IgT teleosts responsible similar functions those carried out by IgA mammals. Moreover, teleost NALT known contain B-cells and nasal mucus contains immunoglobulins (Igs). Yet, whether B...
Abstract Our study explored the effect of oral intubation Bacillus subtilis on Aeromonas hydrophila -induced intestinal mucosal barrier function damage and inflammation in grass carp. The mid-intestine tissue was collected for ATPase activity measurement. Intestinal mucosa also ultrastructurally examined with transmission electron microscope (TEM), its permeability determined using Evans blue (EB) D-lactic acid. pro-inflammation cytokine, MyD88 tight junction (TJ) protein mRNA expression...
Teleost skin serves as the first line of defense against invading pathogens, and contain a skin-associated lymphoid tissue (SALT) that elicit gut-like immune responses antigen stimulation. Moreover, exposed to water environment pathogens therein, teleost is also known be colonized by diverse microbial communities. However, little about interactions between microbiota mucosal system, especially dynamic changes under pathogen infection. We hypothesized dramatic communities strong response...
The skin of vertebrates is the outermost organ body and serves as first line defense against external aggressions. In contrast to mammalian skin, that teleost fish lacks keratinization has evolved operate a mucosal surface containing skin-associated lymphoid tissue (SALT). Thus far, IgT representing prevalent Ig in SALT have only been reported upon infection with parasite. However, very little known about types B cells Igs responding bacterial mucosa, well inductive or effector role such...
Abstract Background The visual organ plays a crucial role in sensing environmental information. However, its mucosal surfaces are constantly exposed to selective pressures from aquatic or airborne pathogens and microbial communities. Although few studies have characterized the conjunctival-associated lymphoid tissue (CALT) ocular mucosa (OM) of birds mammals, little is known regarding evolutionary origins functions immune defense microbiota homeostasis OM early vertebrates. Results Our study...
This study investigates the effect of dietary Lactobacillus plantarum supplementation on juvenile coho salmon (Oncorhynchus kisutch). Four groups juveniles (initial weight 103.87 ± 2.65 g) were fed for 10 weeks with four diets containing 0 (control diet), 105 (T1), 107 (T2), and 109 (T3) cfu/g L. plantarum. The main results are as follows: Compared control diet, final weight, specific growth rate (SGR), gain (WGR) T1, T2, T3 diet significantly (p < 0.05) increased, while feed coefficient...
The buccal mucosa (BM) is a critical first line of defense in terrestrial animals. To gain further insights into the evolutionary origins and primordial roles BM teleosts here we show that rainbow trout, teleost fish, contains diffuse mucosal associated lymphoid tissue (MALT) within its cavity. Upon parasite infection, fish immunoglobulin specialized immunity (sIgT) was induced to high degree, parasite-specific sIgT responses were mainly detected mucus. Moreover, trout microbiota prevalently...
The pharyngeal organ is located at the crossroad of respiratory and digestive tracts in vertebrate, it continuously challenged by varying Ags during breathing feeding. In mammals, mucosa (PM) a critical first line defense. However, evolutionary origins ancient roles immune defense microbiota homeostasis PM are still unknown. this study, to our knowledge, we find that diffuse MALT present rainbow trout, an early vertebrate. Importantly, following parasitic infection, detect strong...
The air-filled organs (AOs) of vertebrates (lungs and swim bladders) have evolved unique functions (air-breathing or buoyancy control in water) to adapt different environments. Thus far, immune responses microbes AOs been described exclusively the lungs tetrapods. Similar lungs, bladders (SBs) represent a mucosal surface, feature that leads us hypothesize role for SB immunity. In this study, we demonstrate secretory IgT (sIgT) is key immunoglobulin (Ig) responding viral challenge, only Ig...
Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little known about the response gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp and developed model infection with Aeromonas hydrophila via intraperitoneal injection. Our findings indicated both innate adaptive responses were effectively elicited after A. infection. Upon infection, + B cells strongly induced in head...
Cyprinid herpesvirus 2 (CyHV-2) causes herpesviral hematopoietic necrosis (HVHN) disease outbreaks in farmed fish, which leads to serious economic losses worldwide. Although oral vaccination is considered the most suitable strategy for preventing infectious diseases so far there no commercial vaccine available controlling HVNN gibel carp ( C. auratus gibelio ). In present study, we developed first time an against CyHV-2 by using yeast cell surface display technology and then investigated...
Bacterial sepsis caused by Aeromonas hydrophila (A. hydrophila) and infectious spleen kidney necrosis virus disease (ISKNVD) (ISKNV) frequently result in significant mortality among Chinese perch (Siniperca chuatsi). Co-infection of mandarin fish with A. ISKNV occurs from time to time. In this study, a visual detection method for was developed, using loop-mediated isothermal amplification (LAMP) pre-addition hydroxynaphthol blue. Primers amplifying LAMP the same system were designed based on...
Symbiotic microbiota in vertebrates play critical roles establishing and enhancing host resistance to pathogenic infections as well maintaining homeostasis. The interactions mechanisms of commensal microbiota-mediated mucosal immune systems have been extensively studied mammals and, a lesser extent, birds. However, despite several studies emphasizing the role controlling pathogen teleost fish, limited knowledge exists regarding core by which they contribute against viral infections. Our...
Abstract Mammalian studies have demonstrated that B cell immune responses are regulated by mechanistic target of rapamycin complex 1 (mTORC1) signaling. Teleost fish represent the oldest living bony vertebrates contain bona fide cells. So far, whether regulatory mechanism mTORC1 signaling in cells occurred teleost is still unknown. In this study, we developed a model using (RAPA) treatment to inhibit and role support, found inhibition RAPA decreased phagocytic capacity, proliferation, Ig...
Grass carp reovirus (GCRV), one of the most serious pathogens threatening grass (Ctenopharyngodon idella), can lead to hemorrhagic disease (GCHD). Currently, GCRV be divided into three genotypes, but comparison their pathogenic mechanisms and host responses remain unclear. In this study, we utilized Ctenopharyngodon idella kidney (CIK) model infected with conduct comparative studies on genotypes. We observed a cytopathic effect (CPE) in GCRV-I GCRV-III groups, whereas GCRV-II group did not...