A5048195143- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Light effects on plants
- Bioinformatics and Genomic Networks
- Plant Physiology and Cultivation Studies
- Gene expression and cancer classification
- Plant Parasitism and Resistance
- Plant pathogens and resistance mechanisms
- Metabolomics and Mass Spectrometry Studies
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Phytochemicals and Antioxidant Activities
- Nitrogen and Sulfur Effects on Brassica
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Phytoestrogen effects and research
- Gene Regulatory Network Analysis
Yokohama City University
2014-2023
Kihara Institute for Biological Research
2014-2023
RIKEN
1997-2021
RIKEN Center for Sustainable Resource Science
2005-2020
The University of Tokyo
1998-2014
Plant (United States)
2004-2013
Japan Science and Technology Agency
2009
University of Toronto
2009
Chiba University
2009
Joetsu University of Education
2003
Summary The transcriptional factor DREB/CBF (dehydration‐responsive element/C‐repeat‐binding) specifically interacts with the dehydration‐responsive element (DRE)/C‐repeat (CRT) cis ‐acting (A/GCCGAC) and controls expression of many stress‐inducible genes in Arabidopsis . Transgenic plants overexpressing DREB1A showed activated improved tolerance to not only drought, salinity, freezing but also growth retardation. We searched for downstream transgenic using full‐length cDNA microarray...
Summary We analyzed global gene expression in Arabidopsis response to various hormones and related experiments as part of the AtGenExpress project. The experimental agents included seven basic phytohormones (auxin, cytokinin, gibberellin, brassinosteroid, abscisic acid, jasmonate ethylene) their inhibitors. In addition, was investigated hormone‐related mutants during seed germination sulfate starvation. Hormone‐inducible genes were identified from hormone data. effects each relevance lists...
Abstract Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about underlying molecular mechanisms. Using an Affymetrix GeneChip representing approximately 8,300 Arabidopsis genes, we studied comprehensive transcript profiles over 24 h in response to indole-3-acetic acid (IAA) brassinolide (BL). We identified 409 genes as BL inducible, 276 IAA 637 total. These two hormones regulated only 48 common, suggesting that most...
Abstract Brassinosteroids (BRs) are steroidal plant hormones that essential for growth and development. Although insights into the functions of BRs have been provided by recent studies biosynthesis sensitivity mutants, mode action is poorly understood. With use DNA microarray analysis, we identified BR-regulated genes in wild type (WT; Columbia) Arabidopsis BR-deficient mutant, det2. generally responded more potently det2 mutant than WT, they showed only limited response a BR-insensitive...
IRE1 plays an essential role in the endoplasmic reticulum (ER) stress response yeast and mammals. We found that a double mutant of Arabidopsis IRE1A IRE1B (ire1a/ire1b) is more sensitive to ER inducer tunicamycin than wild-type. Transcriptome analysis revealed genes whose induction was reduced ire1a/ire1b largely overlapped those bzip60 mutant. observed active form bZIP60 protein detected wild-type missing ire1a/ire1b. further demonstrated mRNA spliced by stress, removing 23 ribonucleotides...
Summary Molecular genetic and physiological studies on brassinosteroid (BR)‐related mutants of dicot plants have revealed that BRs play important roles in normal plant growth development. However, little is known about the function BR monocots (grasses), except for phenotypic analysis a rice mutant partially insensitive to signaling. To investigate monocots, we identified characterized BR‐deficient rice, dwarf1 ( brd1 ). The showed range abnormalities organ development growth, most striking...
Summary GAMYB is a component of gibberellin (GA) signaling in cereal aleurone cells, and has an important role flower development. However, it unclear how function regulated. We examined the involvement microRNA, miR159, regulation expression cells In no miR159 was observed with or without GA treatment, suggesting that not involved ‐like genes this tissue. expressed tissues other than aleurone, over‐expressors showed similar but more severe phenotypes gamyb mutant. are co‐expressed anthers,...
Abstract Brassinosteroids (BRs) are steroidal plant hormones that essential for growth and development. There is only limited information on where BRs synthesized used. We studied the organ specificity of BR biosynthesis in Arabidopsis, using two different approaches: analyzed expression BR-related genes real-time quantitative reverse transcriptase-polymerase chain reaction, endogenous gas chromatography-mass spectrometry. Before starting this study, we cloned second BR-6-oxidase (BR6ox2)...
Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about underlying molecular mechanisms. We studied expression of IAA5 IAA19 in response to treatment with indole acetic acid (IAA) or brassinolide (BL), most active BR. Exogenous IAA induced these genes quickly transiently, whereas exogenous BL them gradually continuously. also found that a fusion DR5, synthetic auxin element, GUS (beta-glucuronidase) gene was similar...
Abstract Brassinosteroids (BRs) are steroidal plant hormones that essential for growth and development. It has been proposed BRs synthesized via two parallel pathways, the early late C-6 oxidation pathways according to status. The tomato (Lycopersicon esculentum) Dwarf gene encodes a cytochrome P450 shown catalyze of 6-deoxocastasterone castasterone. We isolated an Arabidopsis ortholog (AtBR6ox gene) tomatoDwarf gene. encoded polypeptide characteristics P450s is classified into CYP85 family....
The plant hormone auxin is a master regulator of growth and development. By regulating rates cell division elongation triggering specific patterning events, indole 3-acetic acid (IAA) regulates almost every aspect perception involves the formation ternary complex consisting an F-box protein TIR1/AFB family receptors, molecule, member Aux/IAA co-repressor proteins. In this study, we identified potent antagonist, α-(phenylethyl-2-oxo)-IAA, as lead compound for receptors by in silico virtual...
Summary The plant hormone, auxin, regulates many aspects of growth and development. Despite its importance, the molecular mechanisms underlying action auxin are largely unknown. To gain a more comprehensive understanding primary responses to we analyzed expression genes in Arabidopsis seedlings treated with indole‐3‐acetic acid (IAA) for 15 min. We identified single gene that is downregulated early, 29 upregulated early. Several types typical transcription factors as early genes, suggesting...
PRIMe (http://prime.psc.riken.jp/), the Platform for RIKEN Metabolomics, is a Web site that has been designed and implemented to support research analysis workflows ranging from metabolome transcriptome analysis. The provides access growing collection of standardized measurements metabolites obtained by using NMR, GC-MS, LC-MS, CE-MS, metabolomics tools related analyses (SpinAssign identification means KNApSAcK searches within metabolite databases). In addition, transcriptomics provide...
Summary Plant secondary metabolites are known to facilitate interactions with a variety of beneficial and detrimental organisms, yet the contribution specific fungal pathogens is poorly understood. Here we show that, respect aliphatic glucosinolate‐derived isothiocyanates, toxicity against pathogenic ascomycete Sclerotinia sclerotiorum depends on side chain structure. Genes associated formation camalexin glucosinolate were induced in Arabidopsis thaliana leaves challenged necrotrophic...
Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages development and regulation SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) microarray analysis using wild-type plants SL-deficient/insensitive mutants understand molecular mechanisms underlying SL biosynthesis signaling. We that there is responsiveness Arabidopsis seedlings, which includes...
Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome p450 monooxygenase encoded by CYP72B1 has been implicated in brassinosteroid catabolism well photomorphogenesis. We expressed yeast, coupled with feeding, established the biochemical function to be hydroxylation of BL give 26-hydroxybrassinolide 26-hydroxycastasterone, respectively. Brassinosteroid feeding experiments wild-type Arabidopsis, a null mutant,...
Active brassinosteroids (BRs), such as brassinolide (BL) and castasterone (CS), are growth-promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase (CYP734A1, formerly CYP72B1), encoded by the BAS1 gene, inactivates BRs modulates photomorphogenesis. was identified overexpressed gene responsible for a dominant, BR-deficient mutant, bas1-D. This mutant isolated in an activation-tagged screen designed to identify redundant genes that might not be classic loss-of-function screens....
Plants possess many metabolic genes for the production of a wide variety phytochemicals in tissue-specific manner. However, systems behind diversity and tissue-dependent regulation still remain unknown due to incomplete characterization produced single plant species. Thus, having metabolome dataset addition genome transcriptome information resources would enrich our knowledge secondary metabolism. Here we analyzed phytochemical accumulation during development model Arabidopsis (Arabidopsis...
The unfolded protein response (UPR) is a cellular highly conserved in eukaryotes to obviate accumulation of misfolded proteins the endoplasmic reticulum (ER). Inositol-requiring enzyme 1 (IRE1) catalyzes cytoplasmic splicing mRNA encoding bZIP transcription factors activate UPR signaling pathway. Arabidopsis IRE1 was recently shown be involved bZIP60 mRNA. In present study, we demonstrated that an mutant with defects two paralogs showed enhanced cell death upon ER stress compared and wild...
Despite its importance in plant growth and development, the auxin biosynthetic pathway has remained elusive. In this study, we analyzed hormone series transcriptome data from AtGenExpress Arabidopsis found that aminoethoxyvinylglycine (AVG) had strongest anti-auxin activity. We also identified other effective compounds such as l-amino-oxyphenylpropionic acid (AOPP) through additional screening. These inhibitors shared characteristics they inhibited pyridoxal enzymes and/or aminotransferases....
Auxin is essential for plant growth and development, this makes it difficult to study the biological function of auxin using auxin-deficient mutants. Chemical genetics have potential overcome difficulty by temporally reducing inhibitors. Recently, indole-3-pyruvate (IPyA) pathway was suggested be a major biosynthesis in Arabidopsis thaliana L. indole-3-acetic acid (IAA), most common member family. In pathway, YUCCA, flavin-containing monooxygenase (YUC), catalyzes last step conversion from...
We examined whether auxin/indole-3-acetic acid (Aux/IAA) proteins, which are key players in auxin-signal transduction, involved brassinosteroid (BR) responses. iaa7/axr2-1 and iaa17/axr3-3 mutants showed aberrant BR sensitivity BR-induced gene expression an organ-dependent manner. Two auxin inhibitors were tested terms of Yokonolide B inhibited responses, whereas p-chlorophenoxyisobutyric did not inhibit DNA microarray analysis revealed that 108 genes up-regulated, while only eight...