A5065733171- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Physiology and Cultivation Studies
- Seed Germination and Physiology
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- 14-3-3 protein interactions
- Engineering and Material Science Research
- Tissue Engineering and Regenerative Medicine
- Lipid metabolism and biosynthesis
- Diverse Educational Innovations Studies
- Genetic diversity and population structure
- Nuclear Structure and Function
- Impact of Light on Environment and Health
- Fungal and yeast genetics research
- Peroxisome Proliferator-Activated Receptors
Washington State University
2014-2025
ORCID
2020
Van Andel Institute
2017
American Society for Horticultural Science
2016
Washington University in St. Louis
2000-2008
Sainsbury Laboratory
2007
RIKEN
1999-2003
Joetsu University of Education
1999-2003
University of Washington
1991-2002
Howard Hughes Medical Institute
1999-2000
Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging confer resistance antibiotic kanamycin or herbicide glufosinate have used generate several tens of thousands transformed From these, over 30 dominant mutants with various phenotypes isolated. Analysis a subset shown overexpressed genes are almost always found immediately adjacent inserted...
Summary PCR‐based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis most widely used approach polymorphisms. However, this technique limited to mutations which create or disrupt restriction enzyme recognition site. This paper presents modification where mismatches in PCR primer are polymorphism based on target mutation. useful following known segregating populations and genetic mapping isolated DNAs...
Abstract Single, double, and triple null combinations of Arabidopsis mutants lacking the photoreceptors phytochrome (phy) A (phyA-201), phyB (phyB-5), cryptochrome (cry) 1 (hy4-2.23n) were examined for de-etiolation responses in high-fluence red, far-red, blue, broad-spectrum white light. Cotyledon unhooking, unfolding, expansion, hypocotyl growth, accumulation chlorophylls anthocyanin 5-d-old seedlings measured under each light condition dark. phyA was major photoreceptor/effector most...
Seed germination is regulated by endogenous hormonal cues and external environmental stimuli such as water, low temperature, light. After germination, the young seedling must rapidly establish its root system photoautotrophic capability appropriate to surrounding environment. Light phytohormone abscisic acid (ABA) both regulate seed development, although how light ABA signals are integrated at molecular level not understood. Here, we found that previously described light-signaling component...
The Arabidopsis bas1-D mutation suppresses the long hypocotyl phenotype caused by mutations in photoreceptor phytochrome B (phyB). adult of phyB-4 double mutants mimics that brassinosteroid biosynthetic and response mutants. has reduced levels brassinosteroids accumulates 26-hydroxybrassinolide feeding experiments. basis for mutant is enhanced expression a cytochrome P450 (CYP72B1). phyB-null allele, but not phyA-null mutation, partially cryptochrome-null mutation. Seedlings with BAS1 are...
Active brassinosteroids, such as brassinolide (BL) and castasterone, are growth promoting plant hormones. An Arabidopsis cytochrome p450 monooxygenase encoded by CYP72B1 has been implicated in brassinosteroid catabolism well photomorphogenesis. We expressed yeast, coupled with feeding, established the biochemical function to be hydroxylation of BL give 26-hydroxybrassinolide 26-hydroxycastasterone, respectively. Brassinosteroid feeding experiments wild-type Arabidopsis, a null mutant,...
Plants perceive subtle changes in light quality and quantity through a set of photoreceptors, including phytochromes cryptochromes. Upon perception, these photoreceptors initiate signal transduction pathways leading to photomorphogenic development. Using activation-tagging mutagenesis identify novel light-signaling components, we have isolated gain-of-function mutant, sob1-D (suppressor phytochrome B-4 [phyB-4] dominant), which suppresses the long-hypocotyl phenotype phyB missense allele,...
Active brassinosteroids (BRs), such as brassinolide (BL) and castasterone (CS), are growth-promoting plant hormones. An Arabidopsis cytochrome P450 monooxygenase (CYP734A1, formerly CYP72B1), encoded by the BAS1 gene, inactivates BRs modulates photomorphogenesis. was identified overexpressed gene responsible for a dominant, BR-deficient mutant, bas1-D. This mutant isolated in an activation-tagged screen designed to identify redundant genes that might not be classic loss-of-function screens....
Leaves and flowers begin life as outgrowths from the edges of shoot apical meristems. Stem cell divisions in meristem center replenish cells that are incorporated into organ primordia at periphery leave meristem. Organ boundaries, regions limited growth separate forming organs meristem, serve to isolate these two domains critical for coordination organogenesis maintenance. Boundary formation maintenance poorly understood processes, despite identification a number boundary-specific...
Significance Members of the AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED ( AHL ) family are involved in various plant biological processes. Our findings reveal a molecular model whereby AHLs interact with each other via and prokaryote conserved (PPC)/domain unknown function #296 (DUF296) domain to form homo-/hetero-complexes, possibly trimers. The complex also interacts nuclear proteins macromolecular that modulates growth development. coordinated action requires an AT-hook motif capable...
Members of the ancient land-plant-specific transcription factor AT-Hook Motif Nuclear Localized (AHL) gene family regulate various biological processes. However, relationships among AHL genes, as well their evolutionary history, still remain unexplored. We analyzed over 500 genes from 19 land plant species, ranging early diverging Physcomitrella patens and Selaginella to a variety monocot dicot flowering plants. classified proteins into three types (Type-I/-II/-III) based on number...
Whitefly-transmitted begomoviruses cause serious damage to many economically important food, feed, and fiber crops. Numerous vegetable crops are severely affected chilli leaf curl virus (ChiLCV) is the most dominant widely distributed begomovirus in (Capsicum annuum) throughout Indian subcontinent. Recently, CRISPR-Cas9 technology was used as a means reduce geminivirus replication infected plants. However, this approach shown have certain limitations such evolution of escape mutants. In...
Upon detecting abiotic or biotic stress, plants generally reduce their growth, enabling resources to be conserved and diverted stress response mechanisms. In Arabidopsis thaliana, the AT-hook motif nuclear-localized (AHL) transcription factor family has been implicated in restricting rosette growth stress. However, mechanism by which AHLs repress rosettes is unknown. this study, we establish that SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) other restrict petiole elongation antagonizing...
Summary SOB3 , which encodes a plant‐specific AT‐hook motif containing protein, was identified from an activation‐tagging screen for suppressors of the long‐hypocotyl phenotype weak phyB allele, phyB‐4 . sob3‐D ( suppressor phyB‐4#3 dominant ) overexpressing seedlings have shorter hypocotyls, and as adults develop larger flowers leaves, are delayed in senescence compared with wild‐type plants. At nucleotide level, is closely related to ESCAROLA ESC ), independent screen. overexpression also...
The Arabidopsis thaliana hypocotyl is a robust system for studying the interplay of light and plant hormones, such as brassinosteroids (BRs), in regulation growth development. Since BRs cannot be transported between tissues, their cellular levels must appropriate given developmental fates. BR homeostasis maintained part by transcriptional feedback-regulation loops that control expression key metabolic enzymes, including BR-inactivation enzymes CYP734A1/CYP72B1/BAS1 CYP72C1/SOB7. In this...
Interactions between signaling pathways help guide plant development. In this study, we found that brassinosteroid (BR) converges with SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) to influence both the transcription of genes involved in cell elongation and hypocotyl growth. Specifically, SOB3 mutant phenotypes, which are readily apparent when seedlings grown dim white light, were attenuated by treatment either brassinolide (BL) or BR biosynthesis inhibitor brassinazole (BRZ). Hypocotyls light a...
Leaf and cotyledon expansion in dicotyledonous plants is a light-dependent developmental process. The unique role of phytochrome B has been tested by investigating cotyledons wild-type phytochrome-deficient mutants Arabidopsis thaliana (L.) Heynh. A relatively rapid method for measuring area was developed to quantify growth large populations (average n [greater than or equal to] 100) mutant seedlings under different light chemical treatments. Three-day-old (La-er) seedlings, grown...
Developing seedlings are well equipped to alter their growth in response external factors order maximize chances of survival. SUPPRESSOR OF PHYTOCHROME B4-#3 (SOB3) and other members the AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) family transcription modulate development Arabidopsis (Arabidopsis thaliana) by repressing hypocotyl elongation young growing light. However, molecular mechanism behind how AHLs influence seedling is largely unknown. We have identified genes associated with...
Heterostyly is a breeding system that promotes outbreeding through combination of morphological and physiological floral traits. In Turnera these traits are governed by single, hemizygous S-locus containing just three genes. We report the gene, BAHD, mutated encodes severely truncated protein in self-compatible long homostyle species. Further, mutant possesses T. krapovickasii BAHD allele with point mutation highly conserved domain BAHD acyl transferases. Wild type TkBAHD alleles were...
Abstract Gibberellic acid (GA) promotes germination, stem/hypocotyl elongation, and leaf expansion during seedling development. Using activation-tagging mutagenesis, we identified a mutation, sob2-D (for suppressor of phytochromeB-4 [phyB-4]#2 dominant), which suppresses the long-hypocotyl phenotype phyB missense allele, phyB-4. This mutant is caused by overexpression an APETALA2 transcription factor, SOB2, also called DRN-like. SOB2/DRN-like transcript not detectable in wild-type or adult...