A5014087664- Drug Transport and Resistance Mechanisms
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Pharmacological Effects and Toxicity Studies
- Trace Elements in Health
- Acute Myeloid Leukemia Research
- Polysaccharides and Plant Cell Walls
- Endoplasmic Reticulum Stress and Disease
- Ion channel regulation and function
- Nanoparticle-Based Drug Delivery
- Adenosine and Purinergic Signaling
- Microbial Metabolites in Food Biotechnology
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Receptor Mechanisms and Signaling
- Histone Deacetylase Inhibitors Research
- DNA and Nucleic Acid Chemistry
- Autophagy in Disease and Therapy
- Retinoids in leukemia and cellular processes
- Hepatitis B Virus Studies
- Ion Transport and Channel Regulation
- Pharmacological Receptor Mechanisms and Effects
Slovak Academy of Sciences
2016-2025
Centre of Biosciences of the Slovak Academy of Sciences
2008-2025
Bioscience (Slovakia)
2017-2025
Slovak Research and Development Agency
2008-2012
Institute of Chemistry of the Slovak Academy of Sciences
1998-2008
JC-1, a cationic fluorescent dye when added to living cells, is known be localized exclusively in mitochondria, particularly good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 these organelles leads the formation J-aggregates (with specific red fluorescence emission maximum at 590 nm), which addition typical green J-monomers (emission ∼529 nm). lack ΔΨ depression and decrease J-aggregate formation. Therefore, ratio...
Multidrug resistance (MDR) of cancer tissue is a phenomenon in which cells exhibit reduced sensitivity to large group unrelated drugs with different mechanisms pharmacological activity. Mechanisms that reduce cell damage induced by variety chemicals were found be caused diverse, albeit well-defined, phenotypic alterations. The molecular basis MDR commonly involves overexpression the plasma membrane drug efflux pump - P-glycoprotein (P-gp). This glycoprotein an ABCB1 member ABC transporter...
In previous research, we revealed that murine leukemia cells L1210 with induced expression of P-glycoprotein (P-gp, a membrane drug transporter, product the Abcb1 gene) are better able to withstand endoplasmic reticulum (ER) stress (ERS) than their P-gp negative counterparts. This was associated increased GRP78/BiP and modulation several other proteins active in cellular response ERS (like CHOP, spliced XBP1, 50-kDa ATF6 protein fragment others) positive cells. Wolframin is an ER...
The backbone of therapy for elderly patients with myelodysplastic syndromes and acute myeloid leukemia consists hypomethylating agents 5-aza-2’-deoxycytidine (DAC) 5-azacytidine (AZA). However, resistance frequently emerges during treatment. To investigate the mechanisms resistance, we generated DAC-resistant variants cell lines, MOLM-13 SKM-1, through their prolonged cultivation in increasing concentrations DAC. resistant variants, MOLM-13/DAC SKM-1/DAC, exhibited cross-resistance to...
Xyloglucan endotransglycosylases (XETs) catalyse the breakdown of xyloglucan molecules predominantly by transglycosylation. In this process, fragments cleaved polysaccharide are preferentially transferred to other or their oligosaccharide subunits, with overall retention anomeric configuration glycosidic bond. accordance theory, we propose that cleavage and re-formation bond in involves formation a glycosyl-enzyme intermediate which decomposes transfer glycosyl moiety suitable carbohydrate...
P-glycoprotein (P-gp), also known as ABCB1, is a member of the ABC transporter family proteins. P-gp an ATP-dependent drug efflux pump that localized to plasma membrane mammalian cells and confers multidrug resistance in neoplastic cells. 140-kDa polypeptide glycosylated final molecular weight 170 kDa. Our experimental model used two variants L1210 which overexpression was achieved: either by adaptation parental (S) vincristine (R) or transfection with human gene encoding (T). R T were found...
To map the preferred cleavage sites of xyloglucan endotransglycosylases (XETs; EC 2.4.1.207) along donor substrate chain, we incubated enzymes with tamarind (Tamarindus indica) (donor substrate; ≈ 205kDa; 21µM) plus nonasaccharide [3H]XLLGol (Gal2·Xyl3·Glc3· [3H]glucitol; acceptor 0.6µM). After short incubation times, to minimize multiple cleavages, size 3H-labelled transglycosylation products (determined by gel-permeation chromatography) indicated positions relative non-reducing terminus...
The drug efflux activity of P-glycoprotein (P-gp, a product the mdr1 gene, ABCB1 member ABC transporter family) represents mechanism by which tumor cells escape death induced chemotherapeutics. In this study, we investigated mechanisms involved in effects pentoxifylline (PTX) on P-gp-mediated multidrug resistance (MDR) mouse leukemia L1210/VCR cells. Parental sensitive L1210, and multidrug-resistant cells, L1210/VCR, are characterized overexpression P-gp, were used as experimental models....