A5081841860- Corneal Surgery and Treatments
- Retinal Development and Disorders
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Photoreceptor and optogenetics research
- Hemoglobinopathies and Related Disorders
- Advanced biosensing and bioanalysis techniques
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Amino Acid Enzymes and Metabolism
- Folate and B Vitamins Research
- Blood donation and transfusion practices
- Blood transfusion and management
- COVID-19 Clinical Research Studies
- Mesenchymal stem cell research
- Cellular transport and secretion
- Metabolism and Genetic Disorders
- Blood groups and transfusion
- SARS-CoV-2 detection and testing
- Iron Metabolism and Disorders
- Salivary Gland Disorders and Functions
- Endoplasmic Reticulum Stress and Disease
- Photochromic and Fluorescence Chemistry
- Ethics in Clinical Research
- Silk-based biomaterials and applications
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2023
Azienda Sanitaria Unità Locale di Reggio Emilia
2021-2023
Weatherford College
2023
Azienda USL di Bologna
2020-2022
University of Modena and Reggio Emilia
2018-2021
University of Ulster
2016-2019
University of Parma
2013-2015
Abstract Nanoneedles can target nucleic acid transfection to primary cells at tissue interfaces with high efficiency and minimal perturbation. The corneal endothelium is an ideal for nanoneedle‐mediated RNA interference therapy aimed enhancing its proliferative capacity, necessary regeneration. This work develops a strategy siRNA nanoninjection the human endothelium. deliver p16‐targeting endothelial in vitro without toxicity. nanoinjection of induces p16 silencing increases cell...
Meesmann epithelial corneal dystrophy (MECD) is a rare autosomal dominant disorder caused by dominant-negative mutations within the KRT3 or KRT12 genes, which encode cytoskeletal protein keratins K3 and K12, respectively. To investigate pathomechanism of this disease, we generated phenotypically characterized novel knock-in humanized mouse model carrying severe, MECD-associated, K12-Leu132Pro mutation. Although no overt changes in opacity were detected slit-lamp examination, corneas...
Dysregulated expression of the AGXT2L1 gene has been associated to neuropsychiatric disorders. Recently product was shown possess O ‐phosphoethanolamine phospho‐lyase activity. We here analyze specificity in terms both reaction and substrate. show that enzyme, despite having evolved from a transaminase ancestor, is at least 500‐fold more active as lyase than an aminotransferase. Furthermore, very selective for ‐phosphoethanolamine, strongly discriminating against closely related compounds,...
Abstract Corneal endothelial (CE) dysfunction is the main indication for corneal transplantation, an invasive procedure with several limitations. Developing novel strategies to re-activate CE regenerative capacity is, therefore, of fundamental importance. This goal has proved be challenging as cells (CEnC) are blocked in G0/G1 phase cell cycle vivo and, albeit retaining proliferative vitro, this further hindered by endothelial-to-mesenchymal transition. Herein we investigated mechanisms...
Background: Platelet-Rich Plasma (PRP) induces bone regeneration; however, there is low evidence supporting its efficacy in healing. The lack of a standardized protocol administration represents the main obstacle to use clinical routine for defects’ treatment. purpose this study was characterize PRP and elucidate osteogenic potential. Methods: Platelet count, fibrinogen levels, growth factors concentration were measured obtained by four apheresis procedures. HOB-01-C1, pre-osteocytic cell...
Abstract Background and Objectives The first wave of coronavirus disease‐2019 (COVID‐19) dramatically affected the Transfusion Medicine Unit Azienda Unità Sanitari Locale ‐ Istituto di Ricovero e Cura a Carattere Scientifico (AUSL‐IRCCS) Reggio Emilia, which faced total rearrangement procedures for donors patients. This study aims to assess major implications COVID‐19 on our department, focusing blood transfusion chain therapies, in order support specialists seeking efficient ways face...
Human O-phosphoethanolamine (PEA) phospho-lyase is a pyridoxal 5'-phosphate (PLP) dependent enzyme that catalyzes the degradation of PEA to acetaldehyde, phosphate and ammonia. Physiologically, involved in phospholipid metabolism expressed mainly brain, where its expression becomes dysregulated course neuropsychiatric diseases. Mechanistically, shows remarkable substrate selectivity, strongly discriminating against other amino compounds structurally similar PEA. Herein, we studied under...
The corneal endothelium is the inner mono-layered epithelium, fundamental for preserving hydration and transparency. However, molecular mechanisms that regulate endothelial cells (CEnCs), in particular regarding their proliferative capacity, have been only partially elucidated. CEnCs are quiescent vivo they easily undergo to mesenchymal transition (EnMT) vitro. This study aims analyze behavior expression vitro, either sub-confluent growing (S) or confluent (C) cultures. Primary rabbit human...
Various ocular surface diseases are treated with blood-derived eye drops. Their use has been introduced in clinical practice because of their metabolite and growth factor content, which promotes regeneration. Blood-based drops can be prepared from different sources (i.e., whole blood or platelet apheresis donation), as well protocols (e.g., dilutions freeze/thaw cycles). This variability hampers the standardization and, consequently, evaluation efficacy. Detailing sharing methodological...
Human corneal endothelial cells are organized in a tight mosaic of hexagonal and serve critical function maintaining hydration clear vision. Regeneration the tissue is hampered by its poor proliferative capacity, which partially retrieved vitro, albeit only for limited number passages before undergo mesenchymal transition (EnMT). Although different culture conditions have been proposed order to delay this process prolong cell passages, EnMT has still not fully understood successfully...
β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM periodically receive leucodepleted packed red cells (P-RBCs) stored for maximum 14 days. The aim of this study was compare two alternative routine procedures prepare the optimal P-RBCs product, in order identify differences their content may somehow affect patients' health and quality life (QoL). In method 1, then...
Background: Increasing evidences support a correlation between magnesium (Mg) homeostasis and colorectal cancer (CRC). Nevertheless, the role of Mg its transporters as diagnostic markers in CRC is still matter debate. In this study we combined X-ray Fluorescence Microscopy databases information to investigate possible imbalance CRC. Methods: tissue samples their non-tumoural counterpart from four patients were collected analysed for total level distribution by X-Ray Microscopy. We also...
Failure of a protein to achieve its functional structural state and normal cellular location contributes the etiology pathology heritable human conformational diseases. The autosomal dominant form retinitis pigmentosa (adRP) is an incurable blindness largely linked mutations membrane rod opsin. While mechanisms underlying noxious effects mutated are not completely understood, common feature loss. Here, wild type 39 adRP opsin mutants were subjected mechanical unfolding simulations coupled...
Intraoperative cell salvage reduces the need for allogeneic blood transfusion in complex cancer surgery, but concerns about possibility of it re-infusing cells have hindered its application oncology. We monitored presence on patient-salvaged by means flow cytometry; next, we simulated salvage, followed leucodepletion and irradiation contaminated with a known amount EpCAM-expressing cells, assessing also residual proliferation as well quality salvaged red concentrates (RBCs). observed...
Acute radiodermatitis is a significant complication of cancer radiotherapy, and platelet-based therapies are emerging as potential new treatments.In this report, we present the case patient with head neck undergoing radiotherapy combined monoclonal antibody cetuximab. After 4 weeks treatment, developed cutaneous radiation dermatitis. Despite receiving standard treatment corticosteroids emollient cream, lesion did not improve.cutaneous dermatitis on patient.Topical application platelet gel...
Cryopreservation affects platelets' function, questioning their use for cancer patients. We aimed to investigate the biochemical events that occur over time after thawing optimize transfusion timing and evaluate effect of platelet supernatants on tumor cell behavior in vitro. compared fresh (Fresh-PLT) with Cryopreserved platelets (Cryo-PLT) at 1 h, 3 h 6 thawing. MCF-7 HL-60 cells were cultured Fresh- or Cryo-PLT proliferation, migration, PLT-cell adhesion. noticed a significant impairment...
Plasmapheresis donation is considered safe and well tolerated, although long-term effects need to be clarified. The volumes of anticoagulant (ACD-A) used are variable depend primarily on hematocrit (HCT), total blood processed, amount plasma collected, donor characteristics. To elucidate the effect unit weight setting plasmapheresis efficiency ACD-A distribution, we enrolled male donors undergoing a controlled apheresis process donating 700 g 720 in two different sessions. In parallel,...
To investigate the association between biochemical and blood parameters collected before pandemic in a large cohort of Italian donors with risk infection severe disease. We also focused on differences pre- post-Omicron spread Italy (i.e., post-January 01, 2022) observed associations. conducted an observational study 13750 was using data archived up to 5 years pandemic. A t-test or chi-squared test used compare groups. Hazard ratios 95% confidence intervals for SARS-CoV-2 disease were...