A5075522176- Hearing, Cochlea, Tinnitus, Genetics
- Pancreatitis Pathology and Treatment
- Pancreatic function and diabetes
- Cellular transport and secretion
- Vestibular and auditory disorders
- Marine animal studies overview
- Hearing Loss and Rehabilitation
- Neuroscience and Neuropharmacology Research
- Lysosomal Storage Disorders Research
- Neural dynamics and brain function
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Plant Molecular Biology Research
- Zebrafish Biomedical Research Applications
- Galectins and Cancer Biology
- Ion Channels and Receptors
- Connexins and lens biology
- Autophagy in Disease and Therapy
- Sleep and Wakefulness Research
- Acoustic Wave Phenomena Research
- Phagocytosis and Immune Regulation
- RNA regulation and disease
- Underwater Vehicles and Communication Systems
University of Sheffield
2020-2025
University of Liverpool
2018-2022
NIHR Liverpool Pancreatic Biomedical Research Unit
2018
University of Leicester
2015
Myosin-VIIA (MYO7A) is an unconventional myosin responsible for syndromic (Usher 1B) or nonsyndromic forms of deafness in humans when mutated. In the cochlea, MYO7A expressed hair cells, where it believed to act as motor protein tensioning mechanoelectrical transducer (MET) channels, thus setting their resting open probability ( P o ). However, direct evidence this unique role mature cells lacking. Here, we show that has a distinct being crucial structural integrity bundles. Postnatal...
Abstract The refinement of neural circuits towards mature function is driven during development by patterned spontaneous calcium-dependent electrical activity. In the auditory system, this sensory-independent activity arises in pre-hearing cochlea and regulates survival pathway. However, origin interplay calcium signals cochlear unknown vivo. Here we show how dynamics neuroepithelium live mice shape inner hair cells (IHCs) their afferent synapses. Both IHCs supporting (SCs) generate Calcium...
Dopamine (DA) has long been known to have modulatory effects on vertebrate motor circuits. However, the types of information encoded by supraspinal DAergic neurons and their relationship behavior remain unknown.By conducting electrophysiological recordings from awake, paralyzed zebrafish larvae that can produce behaviorally relevant activity patterns, we show generate two forms output: tonic spiking phasic bursting. Using paired DA neuron motoneuron recordings, further these firing modes are...
Type I spiral ganglion neurons (SGNs) convey sound information to the central auditory pathway by forming synapses with inner hair cells (IHCs) in mammalian cochlea. The molecular mechanisms regulating formation of post-synaptic density (PSD) SGN afferent terminals are still unclear. Here, we demonstrate that brain-specific angiogenesis inhibitor 1 (BAI1) is required for clustering AMPA receptors GluR2-4 (glutamate 2-4) at PSD. Adult Bai1-deficient mice have functional IHCs but fail transmit...
Age-related hearing loss (ARHL) is a very heterogeneous disease, resulting from cellular senescence, genetic predisposition and environmental factors (e.g. noise exposure). Currently, we know little about age-related changes occurring in the auditory sensory cells, including those associated with outer hair cells (OHCs). Using different mouse strains, show that OHCs undergo several morphological biophysical ageing cochlea. Ageing also exhibited progressive of afferent efferent synapses. We...
Giant trypsin-containing endocytic vacuoles are formed in pancreatic acinar cells stimulated with inducers of acute pancreatitis. F-actin envelops and regulates their properties. Endocytic can rupture release content into the cytosol cells. fuse plasma membrane exocytose content.Intrapancreatic activation trypsinogen is an early event hallmark development vacuoles, which form by disconnection transport large post-exocytic structures, only resolvable sites trypsin activity live In present...
We investigated hair-cell regeneration in the zebrafish lateral line following application of ototoxic compound copper. In early-larval (<10 days post-fertilisation), regenerated hair cells drive action potentials (APs) afferent neurons 24 h post-copper treatment (24 hpt). Full neuromasts, organs containing cells, requires ∼48 due to progressive addition and synaptic refinement. older larval zebrafish, are active APs by 48 hpt, which is comparable larvae, but functional recovery their...
Activation of trypsinogen (formation trypsin) inside the pancreas is an early pathological event in development acute pancreatitis. In our previous studies we identified activation within endocytic vacuoles (EVs), cellular organelles that appear pancreatic acinar cells treated with inducers EVs are formed as a result aberrant compound exocytosis and subsequent internalization post-exocytic structures. These can be up to 12 μm diameter actinated (i.e. coated F-actin). Notably, undergo...
Key points The present study aimed to determine the sensory adaptation characteristics of hair cell ribbon synapses in vivo . Hair cells zebrafish lateral line transmit hydrodynamic stimuli posterior ganglion afferent neurons. Excitatory bundle deflections by water‐jet cause glutamate release at with a rapid (phasic) and sustained component, which are likely linked exocytosis distinct vesicle pools. glutamate‐induced increase neuron firing rate adapts over time, is mirrored depression...