A5070487301- Bladder and Urothelial Cancer Treatments
- RNA modifications and cancer
- Urological Disorders and Treatments
- Renal cell carcinoma treatment
- Urinary and Genital Oncology Studies
- Prostate Cancer Treatment and Research
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Innovations in Medical Education
- Inflammatory Biomarkers in Disease Prognosis
- HVDC Systems and Fault Protection
- Surgical Simulation and Training
- Protease and Inhibitor Mechanisms
- Diversity and Career in Medicine
- Renal and Vascular Pathologies
- Pelvic and Acetabular Injuries
- Paleopathology and ancient diseases
- Health Sciences Research and Education
- Histone Deacetylase Inhibitors Research
- Chronic Kidney Disease and Diabetes
- Ureteral procedures and complications
- Kidney Stones and Urolithiasis Treatments
- Prostate Cancer Diagnosis and Treatment
University Medical Centre Mannheim
2020-2025
Heidelberg University
2020-2025
University Hospital Heidelberg
2020-2025
University Hospital Bonn
2016
Small non-coding RNAs (sncRNA; <200 nt) regulate various cellular processes and modify gene expression. Under nutritional, biological or physiochemical stress some mature sncRNAs (e.g. tRNAs) are cleaved into halves (30-50 smaller fragments (18-22 nt); the significance functional role of these tRNA is unknown, but their existence has been linked to carcinogenesis. We used small RNA sequencing determine expression sncRNAs. Subsequently findings were validated for miR-122-5p, miR-142-3p...
For clear cell renal carcinoma (ccRCC) risk-dependent diagnostic and therapeutic algorithms are routinely implemented in clinical practice. Artificial intelligence-based image analysis has the potential to improve outcome prediction thereby risk stratification. Thus, we investigated whether a convolutional neural network (CNN) can extract relevant features from representative hematoxylin eosin-stained slide predict 5-year overall survival (5y-OS) ccRCC. The CNN was trained 5y-OS binary...
To develop and validate an interpretable deep learning model to predict overall disease-specific survival (OS/DSS) in clear cell renal carcinoma (ccRCC).Digitised haematoxylin eosin-stained slides from The Cancer Genome Atlas were used as a training set for vision transformer (ViT) extract image features with self-supervised called DINO (self-distillation no labels). Extracted Cox regression models prognosticate OS DSS. Kaplan-Meier univariable evaluation analyses multivariable of the...
Abstract Bladder cancer poses significant clinical challenges due to its high metastatic potential and poor prognosis, especially when it progresses muscle-invasive stages. Here, we show that the m 6 A reader YTHDC1 is downregulated in bladder negatively correlated with expression of epithelial‒mesenchymal transition genes. The functional inhibition or depletion increased migration invasion urothelial cells. Integrative analysis multimodal sequencing datasets provided detailed insights into...
Zusammenfassung Die Weißlichtzystoskopie ist ein präzises Instrument zur Detektion und Therapie von papillären Blasentumoren. Verschiedene zusätzliche makroskopische Detektionstechniken sind etabliert. Bei einigen dieser, allen voran der PDD oder NBI konnte zusätzlicher Nutzen auf die Blasentumorrezidivraten nachgewiesen werden, sodass Anwendung im Rahmen Diagnostik nach Verfügbarkeit erfolgen sollte. Weitere mikroskopische Klassifizierungstechniken multimodale Verfahren in Entwicklung....
Abstract N6-Methyladenosine (m6A) is the most prevalent internal modification of eukaryotic mRNAs and affects regulation its target transcripts in all aspects mRNA life cycle. Cancer-associated changes m6A epitranscriptomic landscape as well deregulation regulators have been linked to cancer development. However, studies focused on vitro or vivo models antibody-dependent mapping techniques, which limited resolution lack quantitative methylation information. Here, we provide first...
To date, only a single transcriptome-wide m6A sequencing study of clear cell renal carcinoma (ccRCC) has been reported, with no validation so far. Herein, by TCGA analysis the KIRC cohort (n = 530 ccRCC; n 72 normal), an external expression 35 preidentified targets was performed. Further in-depth stratification enabled assessment m6A-driven key targets. Overall survival (OS) and gene set enrichment analyses (GSEA) were conducted to assess their clinical functional impact on ccRCC. In...
To assess the baseline inflammatory markers modified Glasgow Prognostic Score (mGPS), systemic immune-inflammation index (SII), and neutrophile-to-lymphocyte ratio (NLR) as pragmatic tools for predicting response to chemohormonal therapy (docetaxel plus ADT) prognosis in men with metastatic hormone-sensitive prostate cancer (mHSPC).Male patients who received docetaxel at a tertiary university care center between 2014 2019 were screened completion of 6 cycles. NLR, SII, mGPS, overall survival...
Purpose: To evaluate the Comprehensive Complication Index (CCI) for reporting complications in lower urinary tract transurethral procedures and compare it with Clavien–Dindo classification (CDC). Materials Methods: A total of 450 consecutive patients were included into analyses [150 each resection bladder tumors (TURBT), prostate (TURP), enucleation using Tm:YAG, (ThuLEP)]. Complications assessed according to modified CDC. The CCI was calculated a freely accessible online tool. Descriptive...
Background: To create and evaluate a realistic, anatomically accurate, user-friendly bladder phantom for reproducible endourological training purposes endoscope mastery. Materials Methods: The anatomy of full bladders was mapped from human computed tomography datasets. After 3D model development process, content evidence response process (RPE) the were evaluated using system usability scale (SUS), 5-point Likert questionnaires, task execution experienced urologists (U) endoscopy-naive...
Introduction: Systematic evaluations focusing on the perception of body image and social support in relation to quality life (QoL) outcomes patients radical cystectomy (RC) with urinary diversion (UD) are currently lacking. This study investigated relationship between perception, support, QoL bladder cancer who underwent RC UD. Methods: A cross-sectional survey was conducted using validated general oncology tools assess newly implemented assessing support. Body assessed Self-Image Scale,...
Abstract Renal cell carcinoma (RCC) is the deadliest primary genitourinary malignancy typically associated with asymptomatic initial presentation and poorly predictable survival. Next to established risk factors, tumor microenvironment may alter metastatic capacity immune landscape. Due their high concentrations, sulfoglycolipids (sulfatides) were among first well-described antigens in RCC that are worse prognosis. As sulfatide detection routine diagnostics not possible, we aimed test...
METTL3 is the major writer of N6-Methyladenosine (m6A) and has been associated with controversial roles in cancer. This best illustrated urothelial carcinoma bladder (UCB), where was described to have both oncogenic tumor-suppressive functions. Here, we reinvestigated role UCB. knockout reduced phenotype m6A levels UCB cell lines. However, complete depletion METTL3/m6A not achieved due selection cells expressing alternative isoforms. Systematic vulnerability inhibitor response analyses...
Current outcome prediction markers for localized prostate cancer (PCa) are insufficient. The impact of the lipid-modifying Sphingomyelin Phosphodiesterase Acid Like 3B (SMPDL3B) in PCa is unknown. Two cohorts patients with who underwent radical prostatectomy (n = 40, n 56) and benign hyperplasia (BPH) controls 8, 11) were profiled SMPDL3B expression qRT-PCR. Publicly available (Memorial Sloane Kettering Cancer Centre (MSKCC; 131, 29 controls) Genome Atlas (TCGA; 497, 53 controls)) served...
To assess the predictive and prognostic value of changes in longitudinal neutrophile-to-lymphocyte (NLR) ratios men receiving taxane-based chemotherapy for metastatic prostate cancer (PC).Retrospective, unicentric cohort study patients treated with either docetaxel hormone-sensitive PC (mHSPC) or cabazitaxel castration-refractory (mCRPC) at a tertiary referral hospital between 2010 2019. NLR were calculated each cycle. Next, slopes over first three (NLR3) six cycles (NLR6) analysed...
Introduction: The aim of this randomised prospective trial was to evaluate a novel hands-on endourological training programme (HTP) and compare it the standard colloquium (SC). Methods: A new HTP created based on sequence theoretical, video-based, practical elements emphasising contemporary teaching methods. An existing SC in which live operations were attended served as comparison. Medical students enrolled ratio 1:2 (SC:HTP). Objective knowledge questionnaires (5 questions, open answers)...
<b><i>Introduction:</i></b> Upper tract urinary cancer recurrence (UTUCR) after radical cystectomy (RC) is outcome-limiting. Surgical recommendations on intraoperative performance of frozen section analysis (FSA) and management positive ureteral margin (PUM) are lacking. <b><i>Methods:</i></b> 634 RC cases were identified (2010–2018). In patients with PUM, sequential resections up to a negative performed. We investigated the accuracy FSA,...
Introduction: First external validation of the Bladder Complexity Score (BCS) for predicting complex transurethral resection bladder tumours (TURBT). Methods: For BCS calculation, TURBTs performed at our institution between January 2018 and December 2019 were reviewed presence preoperative characteristics listed in Checklist (BCC). Receiver operating (ROC) analysis was used validation. To establish a modified (mBCS) with maximum area under curve (AUC), multivariable logistic regression (MLR)...