A5012839649- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Genomic variations and chromosomal abnormalities
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Congenital Anomalies and Fetal Surgery
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Genetic Neurodegenerative Diseases
- Parvovirus B19 Infection Studies
- Fetal and Pediatric Neurological Disorders
- Reproductive Health and Technologies
- Birth, Development, and Health
- CRISPR and Genetic Engineering
- Neurogenetic and Muscular Disorders Research
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Genomics and Rare Diseases
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Mesenchymal stem cell research
Vrije Universiteit Brussel
2015-2025
Universitair Ziekenhuis Brussel
2016-2025
Bio-Medical Science (South Korea)
1997-2019
Medical Genetics Center
2019
Lahey Hospital and Medical Center
2017
Research Centre for Medical Genetics
2015
Institut National de la Recherche Agronomique du Niger
2011
Normandie Université
2011
Université de Caen Normandie
2011
Weatherford College
2010
The seventh report of the ESHRE PGD Consortium is presented documenting cycles collected for calendar year 2004 and follow-up pregnancies babies born subsequent to these up October 2005. Since beginning data collections, there has been a steady increase in number cycles, reported. For collection VII, 45 centres have participated, reporting on 3358 oocyte retrieval (OR), 679 528 born. Five hundred fifty nine OR were reported chromosomal abnormalities, 113 sexing X-linked diseases, 520...
BACKGROUND: Human embryonic stem (hES) cells are pluripotent usually derived from the inner cell mass (ICM) of blastocysts. Because their ability to differentiate into all three germ layers, hES represent an important material for studying developmental biology and replacement therapy. lines blastocysts diagnosed as carrying a genetic disorder after PGD in vitro disease models. METHODS: ICMs isolated by immunosurgery human donated research IVF cycles were plated serum-free medium (except...
In 2005, the European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium published a set of Guidelines Best Practice to give information, support guidance potential, existing fledgling programmes. The subsequent years have seen introduction number new technologies as well evolution current techniques. Additionally, in light recent advice from ESHRE on how practice guidelines should be written formulated, believed it was timely revise update guidelines. Rather than one...
Genomic imbalances are a major cause of constitutional and acquired disorders. Therefore, aneuploidy screening has become the cornerstone preimplantation, prenatal postnatal genetic diagnosis, as well routine aspect diagnostic workup many Recently, array comparative genomic hybridization (array CGH) been introduced rapid high-resolution method for detection both benign disease-causing copy-number variations. Until now, CGH performed using significant quantity DNA derived from pool cells....
Preimplantation genetic diagnosis or screening (PGD, PGS) involves embryo biopsy on Day 3. Opting for one- two-cell is a balance between the lowest risk misdiagnosis one hand and highest chance pregnancy other hand.A prospective controlled trial was designed 592 ICSI cycles were randomly assigned to one-cell (group I) group II). Primary outcomes diagnostic efficiency embryonic development delivery with live birth (analysed by cycle). The false-positive rate PCR presented as secondary outcome...
Since 2004, there have been 11 randomized controlled trials (RCTs) mainly for advanced maternal age (AMA), which shown no benefit of performing preimplantation genetic screening (PGS). Ten the RCTs performed at cleavage stage and one blastocyst stage. It is probable that high levels chromosomal mosaicism stages, may result in tested cell not being representative embryo, inability to examine all chromosomes using fluorescence situ hybridization, contributed lack positive outcome from RCTs. We...
Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future development and implantation capacity highly debated.In order to explore this issue further, a cohort Day 5 single transfers was analysed prospectively embryological clinical outcome. All transferred embryos resulted from 8-cell 3, which subsequently either one cell (group I, n = 182) two cells II, 259) were removed, no invasion by means III, control...
Preimplantation genetic diagnosis (PGD) and subsequently preimplantation screening (PGS) have been introduced since 1990. The difference from the already existing in vitro fertilization (IVF) technology, using intracytoplasmic sperm injection (ICSI), was embryo biopsy at day 3 after fertilization. Although healthy children post-PGD/PGS born, question of whether could any harmful effects has to be studied on large series a prospective manner. A cohort study undertaken 1992 until 2005, same...
The field of preimplantation genetic testing (PGT) is evolving fast, and best practice advice essential for regulation standardisation diagnostic testing. previous ESHRE guidelines on diagnosis, published in 2005 2011, are considered outdated the development new papers outlining recommendations good PGT was necessary. current updated version is, similar to 2011 version, split into four documents, one which covers organisation a centre. other documents focus different technical aspects embryo...
How does the data collection XIV-XV of European Society Human Reproduction and Embryology (ESHRE) PGD Consortium compare with cumulative for collections I-XIII?The 14th 15th retrospective represents valuable on PGD/PGS cycles, pregnancies children: main trend observed is increased application array technology at cost FISH testing in PGS cycles chromosomal abnormalities.Since 1999, has collected, analysed published 13 previous sets an overview first 10 years collections.Data were collected...
The 10th report of the European Society Human Reproduction and Embryology (ESHRE) PGD Consortium is presented, documenting cycles collected for calendar year 2007 follow-up pregnancies babies born until October 2008 which resulted from these cycles. Since beginning data collections there has been a steady increase in number cycles, reported annually. For collection X, 57 centres participated, reporting on 5887 to oocyte retrieval (OR), along with details 1516 1206 born. A total 729 OR were...
The field of preimplantation genetic testing (PGT) is evolving fast and best practice advice essential for regulation standardisation diagnostic testing. previous ESHRE guidelines on PGD, published in 2005 2011, are considered outdated, the development new papers outlining recommendations good PGT was necessary. current paper provides technical aspects monogenic/single-gene defects (PGT-M) covers basic methods PGT-M strategies. Furthermore, some specific formulated special cases, including
Abstract The field of preimplantation genetic testing (PGT) is evolving fast, and best practice advice essential for regulation standardisation diagnostic testing. previous ESHRE guidelines on PGD, published in 2005 2011, are considered outdated, the development new papers outlining recommendations good PGT was necessary. current paper provides technical aspects embryo biopsy covers procedure, cryopreservation laboratory issues training, addition to strengths limitations specific currently...
Abstract The field of preimplantation genetic testing (PGT) is evolving fast, and best practice advice essential for regulation standardisation diagnostic testing. previous ESHRE guidelines on PGD, published in 2005 2011, are considered outdated, the development new papers outlining recommendations good PGT was necessary. current paper provides technical aspects chromosomal structural rearrangements (PGT-SR) aneuploidies (PGT-A) covers array-based comparative genomic hybridisation (aCGH)...
Abstract STUDY QUESTION What are the trends and developments in pre-implantation genetic testing (PGT) 2016–2017 as compared to previous years? SUMMARY ANSWER The main observed this 19th 20th data set on PGT that trophectoderm biopsy has become stage for aneuploidies (PGT-A) implementation of comprehensive technologies is most advanced with PGT-A. WHAT IS KNOWN ALREADY Since it was established 1997, ESHRE Consortium been collecting analysing from mainly European centres. To date, 18 sets an...
Abstract STUDY QUESTION How should ART/preimplantation genetic testing (PGT) centres manage the detection of chromosomal mosaicism following PGT? SUMMARY ANSWER Thirty good practice recommendations were formulated that can be used by ART/PGT as a basis for their own policy with regards to management ‘mosaic’ embryos. WHAT IS KNOWN ALREADY The use comprehensive chromosome screening technologies has provided variety data on incidence at preimplantation stage development and evidence is...
ABSTRACT STUDY QUESTION What are the trends and developments in preimplantation genetic testing (PGT) 2018 as compared to previous years? SUMMARY ANSWER The main observed this 21st dataset on PGT that implementation of trophectoderm biopsy with comprehensive whole-genome is most often applied for PGT-A concurrent PGT-M/SR/A, while PGT-M PGT-SR, single-cell PCR FISH still prevail. WHAT IS KNOWN ALREADY Since it was established 1997, ESHRE Consortium has been collecting analysing data from...
Background/Objectives: Preimplantation genetic testing methods to detect aneuploidy (PGT-A) based on genomewide single nucleotide polymorphism (SNP) data were scarce and did not meet our needs. Methods: Hence, we developed a novel method for this purpose. After the raw B-allele frequency (rBAF) values of Single Nucleotide Polymorfisms (SNPs) are obtained from sample interest with SNP array, BAF specific categories SNPs (cBAF) visualized separately. Results: The analysis cBAF, rBAF Log2R...
The scarcity of genomic DNA can be a limiting factor in some fields genetic research. One the methods developed to overcome this difficulty is whole genome amplification (WGA). Recently, multiple displacement (MDA) has proved very efficient WGA small samples and pools cells, reaction being catalyzed by phi29 or Bst polymerases. aim present study was develop reliable, efficient, fast protocol for MDA at single-cell level. We first compared efficiency polymerases on single cells. polymerase...