A5051476729- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Renal and related cancers
- Pluripotent Stem Cells Research
- Reproductive System and Pregnancy
- Seed Germination and Physiology
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Diversity and Career in Medicine
- Spectroscopy Techniques in Biomedical and Chemical Research
- Gene expression and cancer classification
- Genetic and phenotypic traits in livestock
- Growth Hormone and Insulin-like Growth Factors
- Endometrial and Cervical Cancer Treatments
- Educational and Social Studies
- Uterine Myomas and Treatments
- DNA Repair Mechanisms
- Ectopic Pregnancy Diagnosis and Management
- Maternal and Neonatal Healthcare
Digital Research Alliance of Canada
2024
Biogen (Italy)
2010-2018
Istituti Clinici di Perfezionamento
2010-2017
Istituti Clinici Scientifici Maugeri
2011-2014
Azienda Ospedaliera San Gerardo
2011
International Centre for Reproductive Health
2006-2010
University of Bologna
2004-2006
Marche Polytechnic University
2006
Westmead Hospital
2000
The University of Sydney
2000
BACKGROUND: The relationship between early embryo post-implantation development in couples undergoing assisted reproductive techniques (ARTs) and sperm chromatin alterations has not been satisfactorily explained. aim of this study was to assess the DNA fragmentation IVF/ICSI patients, parameters (concentration, motility morphology) ART outcome, especially with regard clinical pregnancy loss (spontaneous miscarriage or biochemical pregnancy). METHODS: evaluated by TUNEL assay, performed on...
What recommendations can be provided on the approach to and use of time-lapse technology (TLT) in an IVF laboratory?The present ESHRE document provides 11 how introduce TLT laboratory.Studies have been published clinical embryology. However, a systematic assessment this is currently missing.A working group members Steering Committee Special Interest Group Embryology selected was formed order write practical aspects for laboratory.The included different nationalities with internationally...
BACKGROUND: Recently, interest in oocyte cryopreservation has steadily increased. Newly developed protocols have dramatically improved survival rates, removing perhaps the major hurdle that prevented this approach from becoming a fully established form of treatment. However, clinical efficiency these not been exhaustively explored and therefore remains controversial. METHODS: Morphologically normal oocytes displaying first polar body were frozen–thawed with slow cooling protocol utilized 1.5...
Oocyte in vitro maturation (IVM) has become a valuable technological tool for animal breeding and cloning the treatment of human infertility because it does not require administration exogenous gonadotropin to obtain fertilizable oocytes. However, embryo development after IVM is lower compared vivo maturation, most likely oocytes collected are heterogeneous with respect their developmental competencies. Attempts improve outcome have relied upon either prematuration culture (PMC) or two-step...
Can focused application of time-lapse microscopy (TLM) lead to a more detailed map the morphokinetics human fertilization, revealing novel or neglected aspects this process?Intensive harnessing TLM reveals previously poorly characterised phenomena such as cytoplasmic wave (CW) preceding pronuclear formation and kinetics chromatin polarization, thereby suggesting non-invasive biomarkers embryo quality.In recent years, preimplantation development has been object studies with intent develop...
Abstract The field of preimplantation genetic testing (PGT) is evolving fast, and best practice advice essential for regulation standardisation diagnostic testing. previous ESHRE guidelines on PGD, published in 2005 2011, are considered outdated, the development new papers outlining recommendations good PGT was necessary. current paper provides technical aspects embryo biopsy covers procedure, cryopreservation laboratory issues training, addition to strengths limitations specific currently...
Abstract STUDY QUESTION How should ART/preimplantation genetic testing (PGT) centres manage the detection of chromosomal mosaicism following PGT? SUMMARY ANSWER Thirty good practice recommendations were formulated that can be used by ART/PGT as a basis for their own policy with regards to management ‘mosaic’ embryos. WHAT IS KNOWN ALREADY The use comprehensive chromosome screening technologies has provided variety data on incidence at preimplantation stage development and evidence is...
Preimplantation genetic testing for aneuploidies (PGT-A) is arguably the most effective embryo selection strategy. Nevertheless, it requires greater workload, costs, and expertise. Therefore, a quest towards user-friendly, non-invasive strategies ongoing. Although insufficient to replace PGT-A, morphological evaluation significantly associated with embryonic competence, but scarcely reproducible. Recently, artificial intelligence-powered analyses have been proposed objectify automate image...
Abstract STUDY QUESTION Does maternal ageing impact early and late morphokinetic cellular processes of human blastocyst formation? SUMMARY ANSWER Maternal significantly affects pronuclear size intra- extra-nuclear dynamics during fertilization, dysregulates cell polarity compaction, reduces blastocoel expansion. WHAT IS KNOWN ALREADY In ART, advanced age (AMA) oocyte yield, overall developmental competence. However, with the exception chromosome segregation errors occurring meiosis,...
One of the major concerns derived from cryopreservation meiotically mature oocytes is possible damage to cytoskeletal apparatus, and in particular meiotic spindle.One hundred fresh showing polar body I high spindle birefringence (maximum retardance+/-1.5 mol/l SD = 2.58+/-0.1 nm), assessed through analysis, were included this study. Oocytes cryopreserved with a 1.5 1,2-propanediol +0.3 sucrose solution. After thawing, spindles imaged at 0, 3 5 h. Spindle was quantified by measuring...
BACKGROUNDThe demand for cryopreservation of human oocytes is increasing in assisted reproduction clinics and yet remains an experimental procedure. Surprisingly, little known about the effects on spindle–chromosome interactions recovery meiotic spindle functionality. The goal these studies was to evaluate process reassembly chromosome alignment cryopreserved metaphase II oocytes.
We studied the ultrastructural characteristics of human mature oocytes frozen/thawed (F/T) using different concentrations sucrose. Fresh were used as controls. The (n = 48) fixed in 1.5% glutaraldehyde at sampling 16) or after freeze/thawing performed a slow cooling method with propane-1,2-diol 1.5 mol/l and sucrose either 0.1 0.3 freezing solution. then processed for electron microscopy observations. F/T belonging to both study groups regularly rounded sections, homogeneous cytoplasm an...