A5066371582- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Cardiovascular Syncope and Autonomic Disorders
- Shoulder Injury and Treatment
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Genital Health and Disease
- Mitochondrial Function and Pathology
- Veterinary Equine Medical Research
- Proteoglycans and glycosaminoglycans research
- Dental Trauma and Treatments
- Lower Extremity Biomechanics and Pathologies
- Rheumatoid Arthritis Research and Therapies
- Planarian Biology and Electrostimulation
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Nasal Surgery and Airway Studies
- Oral and Maxillofacial Pathology
- Muscle Physiology and Disorders
- Corneal Surgery and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Sports injuries and prevention
University of Malaya
2011-2023
Abstract Chondrogenic differentiated mesenchymal stem cells (CMSCs) have been shown to produce superior chondrogenic expression markers in vitro. However, the use of these vivo has not fully explored. In this study, assessment cartilage repair potential between allogenic‐derived pre‐differentiated and undifferentiated MSCs (MSCs) were compared. Bilateral full thickness defects created on medial femoral condyles 12 rabbits ( n = 12). Rabbits divided into two groups. one group, right knees...
Background: Mesenchymal stem cells (MSCs) represent a promising alternative form of cell-based therapy for cartilage injury. However, the capacity MSCs chondrogenesis has not been fully explored. In particular, there is presently lack studies comparing effectiveness to conventional autologous chondrocyte (autoC) treatment regeneration full-thickness defects in vivo. Hypothesis: Treatment with allogenic undifferentiated (alloMSCs) results superior tissue profiles when compared autoC repair...
The use of growth differentiation factor 5 (GDF-5) in damaged tendons has been shown to improve tendon repair. It hypothesized that further improvements may be achieved when GDF-5 is used promote cell proliferation and induce tenogenic human bone marrow-derived mesenchymal stem cells (hMSCs). However, the optimal conditions required produce these effects on hMSCs have not demonstrated previous studies. A study determine exposed different concentrations (0, 5, 25, 50, 100 500 ng/ml) was thus...
Monosodium -iodoacetate (MIA)-induced animal model of osteoarthritis (OA) is under-utilised despite having many inherent advantages.At present, there lack studies that directly compare the degenerative changes induced by MIA with surgical induction method and human osteoarthritis, which would further verify a greater use this model.Therefore, we compared histological, biochemical biomechanical characteristics in rat using against anterior cruciate ligament transection (ACLT) cartilage...
Abstract Background In many pre-clinical studies of cartilage tissue, it has been generally assumed that the major difference tissue between species is thickness, which related to size animal itself. At present, there appear be lack demonstrating relationship chondrocyte densities, protein content, gene expressions and thickness in various models are commonly used. The present study was conducted determine whether or not density, proteoglycan/protein content selective expression merely (thus...
Abstract Human amniotic membrane (HAM) is an established biomaterial used in many clinical applications. However, its use for tissue engineering purposes has not been fully realized. A study was therefore conducted to evaluate the feasibility of using HAM as a chondrocyte substrate/carrier. HAMs were obtained from fresh human placenta and process produced air dried (AdHAM) freeze (FdHAM). Rabbit chondrocytes isolated expanded vitro seeded onto these preparations. Cell proliferation, GAG...
Previous studies have shown that platelet concentrates used in conjunction with appropriate growth media enhance osteogenic differentiation of human mesenchymal stromal cells (hMSCs). However, their potential inducing osteogenesis hMSCs when cultured serum free medium has not been explored. Furthermore, the resulting molecular signatures compared to standard medium. We studied effect infrequent supplementation (8-day interval) 15% non-activated platelet-rich concentrate (PRC) on...
Platelet-rich concentrate (PRC), used in conjunction with other chondroinductive growth factors, have been shown to induce chondrogenesis of human mesenchymal stromal cells (hMSC) pellet culture. However, culture systems promote cell hypertrophy and the presence factors media previous studies obscures accurate determination effect platelet itself inducing chondrogenic differentiation. Hence, this study aimed investigate PRC alone enhancing differentiation potential encapsulated...
It has been suggested that mechanical strain may elicit cell differentiation in adult somatic cells through activation of epithelial sodium channels (ENaC). However, such phenomenon not previously demonstrated mesenchymal stromal (MSCs). The present study was thus conducted to investigate the role ENaC human bone marrow-derived MSCs (hMSCs) tenogenic during uniaxial tensile loading. Passaged-2 hMSCs were seeded onto silicone chambers coated with collagen I and subjected stretching at 1 Hz...
Platelet rich concentrate (PRC) is a natural adjuvant that aids in human mesenchymal stromal cell (hMSC) proliferation vitro ; however, its role requires further exploration. This study was conducted to determine the optimal concentration of PRC required for achieving maximal proliferation, and need activating platelets achieve this effect, if could independently induce early differentiation hMSC. The gene expression markers osteocytes (ALP, RUNX2), chondrocytes (SOX9, COL2A1), adipocytes...
Streptokinase is the thrombolytic therapy of choice for acute myocardial infarction in hospitals without cardiac facilities. Streptokinase‑induced hypotension one common adverse drug reactions and usually observed within 30 min an intravenous streptokinase infusion. The present study aimed to identify predictive clinical parameters development patients with infarction. retrospective involved data transcription from National Indicator Approach (NIA) records recorded between 2015 2018....