A5027457879- Retinopathy of Prematurity Studies
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neonatal and fetal brain pathology
- Photoreceptor and optogenetics research
- Neonatal Respiratory Health Research
- Glaucoma and retinal disorders
- Ophthalmology and Visual Impairment Studies
- Retinal and Optic Conditions
- Traumatic Brain Injury and Neurovascular Disturbances
- Photoacoustic and Ultrasonic Imaging
- Retinal Imaging and Analysis
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Corneal surgery and disorders
- Birth, Development, and Health
- Retinal and Macular Surgery
- Pregnancy and preeclampsia studies
- Intraocular Surgery and Lenses
- Drug-Induced Ocular Toxicity
- Preterm Birth and Chorioamnionitis
- Fibroblast Growth Factor Research
- Circadian rhythm and melatonin
- Fatty Acid Research and Health
- Wnt/β-catenin signaling in development and cancer
Boston Children's Hospital
2015-2024
Harvard University
2015-2024
Northeastern University
2002-2022
Boston Children's Museum
2008-2015
Children's Hospital
2009-2011
Ophthalmology Associates (United States)
2010
Pathological neovessels growing into the normally avascular photoreceptors cause vision loss in many eye diseases, such as age-related macular degeneration and telangiectasia. Ocular neovascularization is strongly associated with inflammation, but source of inflammatory signals mechanisms by which these regulate disruption privilege are unknown. In this study, we found that c-Fos, a master regulator, was increased model pathological blood vessels invading photoreceptors: very low-density...
Retinal neuronal abnormalities occur before vascular changes in diabetic retinopathy. Accumulating experimental evidence suggests that neurons control pathology and other neovascular retinal diseases. Therefore, normalizing activity diabetes may prevent pathology. We investigated whether fibroblast growth factor 21 (FGF21) prevented dysfunction insulin-deficient mice. found neural retina, photoreceptor rather than inner function was most affected administration of the long-acting FGF21...
To test the hypothesis that early rod dysfunction predicts blood vessel abnormalities are clinical hallmark of retinopathy prematurity (ROP).Two rat models ROP, induced by exposure to alternating 50%/10% oxygen (50/10 model) from postnatal day (P) 0 P14, or 75% (75 P7 and controls reared in room air were studied. In a longitudinal design, electroretinographic (ERG) records digital fundus images obtained at P20 +/- 1, P30 P60 1. Rod sensitivity was derived ERG a-wave. Integrated curvature for...
In a rat model of retinopathy prematurity (ROP), the retinal vasculature and function neural retina were studied longitudinally. Vascular parameters evaluated for significant relationships.Retinopathy was induced by exposing newborn rats to alternating 50% 10% oxygen until age 14 days. To evaluate retina, electroretinographic (ERG) responses full-field stimuli recorded from dark-adapted at ages 18 31 Sensitivity saturated amplitude photoreceptor postreceptor activity derived ERG a- b- waves....
Diabetic retinopathy (DR) is associated with hyperglycemia-driven microvascular pathology and neuronal compromise in the retina. However, DR also linked to dyslipidemia. As omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) are protective proliferative retinopathy, we investigated capacity of ω-3PUFAs preserve retinal function a mouse model type 2 diabetes mellitus (T2DM).Male leptin-receptor-deficient (db/db) mice were maintained for 22 weeks (4 weeks-26 life) on calorically compositionally...
The neural cells and factors determining normal vascular growth are not well defined even though vision-threatening neovessel growth, a major cause of blindness in retinopathy prematurity (ROP) (and diabetic retinopathy), is driven by delayed growth. We here examined whether hyperglycemia low adiponectin (APN) levels retinal vascularization, primarily dysregulated photoreceptor metabolism. In premature infants, APN correlated with formation. Experimentally neonatal mouse model postnatal...
There is a gap in understanding the effect of essential ω-3 and ω-6 long-chain polyunsaturated fatty acids (LCPUFA) on Phase I retinopathy prematurity (ROP), which precipitates proliferative ROP. Postnatal hyperglycemia contributes to ROP by delaying retinal vascularization. In mouse neonates with hyperglycemia-associated retinopathy, dietary (vs. LCPUFA) supplementation promoted vessel development. However, was also developmentally essential, promoting neuronal growth metabolism as...
To study the development of electroretinographic (ERG) oscillatory potentials (OPs) in two rat models ROP and human subjects with a history ROP.Sprague-Dawley rats (n = 36) were studied longitudinally. Rat induced, either by exposure to alternating 50%/10% oxygen (50/10 model) from postnatal day (P) 0 P14 or 75% (75 P7 P14. Control reared room air. Infant adult without 91) also studied. Dark-adapted ERGs recorded filtered demonstrate OPs. Discreet Fourier transform (DFT) allowed evaluation...
Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are complementary imaging modalities, the combination of which can provide clinicians with a wealth information to detect retinal diseases, monitor disease progression, or assess new therapies. Adaptive optics (AO) is tool that enables correction wavefront distortions from ocular aberrations. We have developed multimodal adaptive system (MAOS) for high-resolution multifunctional use in variety research clinical...
The blood-retinal barrier (BRB) prevents most systemically-administered drugs from reaching the retina. This study investigated whether burst ultrasound applied with a circulating microbubble agent can disrupt BRB, providing noninvasive method for targeted delivery of systemically administered to To demonstrate efficacy and reversibility such procedure, five overlapping targets around optic nerve head were sonicated through cornea lens in 20 healthy male Sprague-Dawley rats using 690 kHz...
Purpose: Because preterm birth and retinopathy of prematurity (ROP) are associated with poor visual acuity (VA) altered foveal development, we evaluated relationships among the central retinal photoreceptors, postreceptor neurons, overlying fovea, VA in ROP. Methods: We obtained optical coherence tomograms (OCTs) born subjects no history ROP (none; n = 61), that resolved spontaneously without treatment (mild; 51), required by laser ablation avascular peripheral retina (severe; 22), as well...
purpose. To study the development of electroretinographic (ERG) oscillatory potentials (OPs) in rats and to compare normal OPs with those a rat model retinopathy prematurity (ROP). methods. Following longitudinal design, ERG responses greater than 5 log unit range full-field stimuli were recorded dark-adapted at postnatal day (P) 18, P31, P47, P67. The records digitally filtered (60–235 Hz), trough-to-peak amplitudes implicit times OP2, OP3, OP4, OP5 measured. Additionally, oxygen-induced...
purpose. To evaluate rod photoreceptor and postreceptor retinal function in pediatric patients with achromatopsia (ACHR) blue cone monochromatism (BCM) using contemporary electroretinographic (ERG) procedures. methods. Fifteen (age range, 1–20 years) ACHR six 4–22 BCM were studied. ERG responses to full-field stimuli obtained scotopic photopic conditions. Rod (S , R ) rod-driven (log σ, V max response parameters calculated from the a-wave b-wave. records digitally filtered demonstrate...
Pathological neovascularization is a hallmark of late stage neovascular (wet) age-related macular degeneration (AMD) and the leading cause blindness in people over age 50 western world. The treatments focus on suppression choroidal (CNV), while current approved therapies are limited to inhibiting vascular endothelial growth factor (VEGF) exclusively. However, this treatment does not address underlying AMD, loss VEGF's neuroprotective can be potential side effect. Therapy which targets key...
Adaptive optics provides improved resolution in ophthalmic imaging when retinal microstructures need to be identified, counted, and mapped. In general, multiple images are averaged improve the signal-to-noise ratio or analyzed for temporal dynamics. Image registration by cross-correlation is straightforward small patches; however, larger require more sophisticated techniques. Strip-based has been used successfully photoreceptor mosaic alignment if deformations along strips not simple...
Purpose.: Dopamine (DA) is a neurotransmitter implicated both in modulating neural retinal signals and eye growth. Therefore, it may participate the pathogenesis of most common clinical sequelae retinopathy prematurity (ROP), visual dysfunction myopia. Paradoxically, ROP myopia usually small. The rat with oxygen-induced (OIR) characterized by short axial length. There have been several investigations early maturation DA retina, but little at older ages, not OIR rat. DA, function, refractive...
We generated a model of eye growth and tested it against an known to develop abnormally, one with history retinopathy prematurity (ROP).We reviewed extant magnetic resonance images (MRIs) from term preterm-born patients for suitable (n = 129). binned subjects analysis based upon postmenstrual age at birth (in weeks) ROP ("Term" ≥ 37, "Premature" ≤ 32 no ROP, "ROP" ROP). measured the axial positions curvatures cornea, anterior posterior lens, inner retinal surface. fit chamber depth (ACD),...