Abstract Using low cost and small size light emitting diodes (LED) as the alternative illumination source for photoacoustic (PA) imaging has many advantages, can largely benefit clinical translation of emerging PA technology. Here, we present our development LED-based integrated with B-mode ultrasound. To overcome challenge achieving sufficient signal-to-noise ratio by LED that is orders magnitude weaker than lasers, extensive signal averaging over hundreds pulses performed. Facilitated fast...

Changes of physiological pH are correlated with several pathologies, therefore the development more effective medical imaging methods is paramount importance. Here, we report on an in vivo mapping nanotechnology. This subsurface chemical based tumor-targeted, sensing nanoprobes and multi-wavelength photoacoustic (PAI). The nanotechnology consists optical indicator, SNARF-5F, 5-(and-6)-Carboxylic Acid, encapsulated into polyacrylamide nanoparticles surface modification for tumor targeting....

Abstract By using our dual-modality system enabling simultaneous real-time ultrasound (US) and photoacoustic (PA) imaging of human peripheral joints, we explored the potential contribution PA modality to rheumatology clinic. performing at a single laser wavelength, spatially distributed hemoglobin content reflecting hyperemia in synovial tissue metacarpophalangeal (MCP) joints 16 patients were imaged, compared results from healthy controls. In addition, by two wavelengths, oxygenation...

Ion-selective optodes (ISOs), the optical analog of ion-selective electrodes, have played an increasingly important role in chemical and biochemical analysis. Here we extend this technique to photoacoustic (ISPAOs) that serve at same time as fluorescence-based ISOs, apply it specifically potassium (K+). Notably, ion is one most abundant cations biological systems, involved numerous physiological pathological processes. Furthermore, has been recently reported presence abnormal extracellular...

We hereby apply the approach of photoacoustic chemical imaging, performing an in vivo analysis that is spatially resolved (200 μm) and real time, to predict a given tumor's response therapy. Using triple negative breast cancer as model, we took images tumors' oxygen distributions patient-derived xenografts (PDXs) mice using biocompatible, oxygen-sensitive tumor-targeted contrast nanoelements (nanosonophores), which function agents for imaging. Following radiation therapy, established...

Lifetime imaging methods using phosphorescence quenching by oxygen for molecular concentration measurement have been developed and used noninvasive monitoring. This study reports photoacoustic (PA) powered polyacrylamide (PAAm) hydrogel nanoparticles (NP) which offer advantages including improved biocompatibility, reduced toxicity, active tumor targeting. A known indicator, oxyphor G2, was conjugated with the matrix of NPs, giving G2-PAA followed PEGylation biocompatibility F3 surface...

Rheumatoid arthritis (RA) is a chronic autoimmune disease that can cause severe joint damage and functional impairment. Ultrasound imaging has shown promise in providing real-time assessment of synovium inflammation associated with the early stages RA. Accurate segmentation region quantification inflammation-specific biomarkers are crucial for assessing grading However, automatic 3D ultrasound challenging due to ambiguous boundaries, variability shape, inhomogeneous intensity distribution....

Aiming at clinical translation, we developed an automatic 3D imaging system combining the emerging photoacoustic with conventional Doppler ultrasound for detecting inflammatory arthritis. This was built a GE HealthCare (GEHC) Vivid™ E95 and Universal Robot UR3 robotic arm. In this work, performance of examined longitudinal study utilizing clinically relevant adjuvant induced arthritis (AIA) murine model. After injection, daily rat ankle joints conducted until joint inflammation obvious based...

Efficient and noninvasive generation of cavitation bubbles in soft tissue is a challenging task due to the lack nuclei (i.e., pre-existing gas bubbles). In this study, we present method generate enhance activity based on utilization two consecutive ultrasound waves at different frequencies. First, high frequency (5 MHz) intensity focused (HIFU) wave was applied tissue-mimicking phantom induce rapid temperature rise focal region. Immediately following HIFU wave, low (1 same region acoustic...

Purpose The combination of laser and ultrasound can significantly improve the efficiency thrombolysis through an enhanced cavitation effect. We developed a fiber optics‐based laser‐ultrasound device tested feasibility this technology for restoring blood flow in vitro clot model. Methods An flow‐clot model was setup, then endovascular system combined with high‐intensity focused to remove clot. pulses were synchronized delivered concurrently. 532 nm endovascularly optical fiber, whereas 0.5...

Photoacoustic microscopy (PAM) was used to detect small animal brain activation in response drug abuse. Cocaine hydrochloride saline solution injected into the blood stream of Sprague Dawley rats through tail veins. The rat functional change injection then monitored by PAM technique. Images coronal view at locations 1.2 and 3.4 mm posterior bregma were obtained. resulted photoacoustic (PA) images showed regional changes volume. Additionally, oxygenation also presented. results demonstrated...

The enhanced heating effect during the combination of high-intensity focused ultrasound (HIFU) and low-optical-fluence laser illumination was investigated by using an in vivo murine animal model. thighs animals were synergistically irradiated HIFU pulsed nano-second light. temperature increases target region measured a thermocouple under different pressures, which 6.2, 7.9, 9.8 MPa, with 20 mJ/cm2 exposures at 532 nm wavelength. In comparison conventional therapies, fluence used here is...

We describe how 4-dimensional in vivo biochemical analysis can be performed using photoacoustic contrast nanoagents that have been designed to probe both structural and chemical information vivo, enabling noninvasive, real time, spatially resolved imaging. Early imaging of a patient's tumor inform the decision effective treatment, regarding choices chemotherapy, radiation, or immunotherapy.

Significance: One key pathological characteristic of seronegative spondyloarthropathy (SpA) is inflammation at the insertion tendons and ligaments into bone (enthesitis). Aim: We explore potential emerging photoacoustic (PA) imaging in diagnosis SpA review its feasibility detecting SpA-associated Achilles tendon enthesitis. Approach: A light-emitting diode (LED)-based PA ultrasound combined system was employed. The images, both along long short axes each region, were acquired 850-nm...

Abstract Purpose This study aimed to test the feasibility of combined ultrasound and laser technique, namely, ultrasound‐assisted endovascular thrombolysis (USELT), for by conducting in vivo tests a rabbit thrombosis model. Materials methods An acute thrombus was created right jugular vein then treated with only, USELT dissolve blood clot. A total 20 rabbits were used. Out which, first three used titrate parameters. Then, five seven USELT. During USELT, 532‐nm pulses delivered endovascularly...

Evaluating the aggressiveness of prostate cancer (PCa) is crucial for PCa diagnosis and prognosis. Previously, studies have shown that photoacoustic spectral analysis (PASA) can assess tissue microarchitecture evaluating PCa. In this study, in a transgenic mouse (TRAMP) model PCa, we utilized methylene blue polyacrylamide nanoparticles (MB PAA NPs) to label cells vivo. MB NPs specifically target proliferating as contrast agent, allowing (PA) imaging better detect tumors, also assessing...

Aiming at a point-of-care device for rheumatology clinics, we developed an automatic 3-D imaging system combining the emerging photoacoustic (PA) with conventional Doppler ultrasound (US) detecting human inflammatory arthritis. This is based on commercial-grade GE HealthCare (GEHC, Chicago, IL, USA) Vivid E95 US machine and Universal Robot UR3 robotic arm. automatically locates patient's finger joints from photograph taken by overhead camera powered hand joint identification method, followed...

Abstract Photoacoustic imaging (PAI) is an emerging modality in biomedical with superior depth and specificity. However, PAI still has significant limitations, such as the background noise from endogenous chromophores. To overcome these we developed a covalent activity‐based probe, NOx‐JS013, targeting NCEH1. NCEH1, highly expressed activated serine hydrolase aggressive cancers, potential to be employed for diagnosis of cancers. We show that NOx‐JS013 labels active NCEH1 live cells high...

Ultrasound imaging has shown promise in assessing synovium inflammation associated early stages of rheumatoid arthritis (RA). The precise identification the and quantification inflammation-specific biomarkers is a crucial aspect accurately quantifying grading RA. In this study, deep learning-based approach presented that automates segmentation ultrasound images finger joints affected by Two convolutional neural network architectures for image were trained validated limited number 2-D images,...

The diagnosis of aggressive prostate cancer (PCa) has relied on microscopic architectures, namely Gleason patterns, tissues extracted through core biopsies. Technology capable assessing the tissue architecture without extraction will reduce invasiveness PCa and improve diagnostic accuracy by allowing for more sampling locations. Our recently developed photoacoustic spectral analysis (PASA) achieved quantification architectural heterogeneity interstitially. Taking advantage unique optical...

The Methylene Blue loaded Polyacrylamide Nanoparticles (MB-PAA NPs) are used for oxygen sensing and Photodynamic therapy (PDT), a promising therapeutic modality employed various tumors, with distinct advantages of delivery biomedical agents protection from other bio-molecules overcoming inherent limitations molecular dyes. Lifetime-resolved photoacoustic spectroscopy using quenched-phosphorescence method is applied MB-PAA NPs so as to sense oxygen, while the same light source PDT. dye...