A5057813092- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Spine and Intervertebral Disc Pathology
- Total Knee Arthroplasty Outcomes
- Silk-based biomaterials and applications
- Collagen: Extraction and Characterization
- Musculoskeletal pain and rehabilitation
- Knee injuries and reconstruction techniques
- Bone Tissue Engineering Materials
- Hydrogels: synthesis, properties, applications
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Anesthesia and Pain Management
- Innovative Microfluidic and Catalytic Techniques Innovation
- Angiogenesis and VEGF in Cancer
- Shoulder Injury and Treatment
- biodegradable polymer synthesis and properties
- Additive Manufacturing and 3D Printing Technologies
- Proteoglycans and glycosaminoglycans research
Eindhoven University of Technology
2015-2023
University Medical Center Utrecht
2015-2018
Utrecht University
2017-2018
Bioprinting of chondrocyte-laden hydrogels facilitates the fabrication constructs with controlled organization and shape e.g. for articular cartilage implants. Gelatin-methacryloyl (gelMA) supplemented gellan gum is a promising bio-ink. However, rheological properties governing printing process, influence on mechanical chondrogenesis blend, are still unknown. Here, we investigated suitability gelMA/gellan bioprinting. Multiple concentrations, ranging from 3% to 20% gelMA 0%–1.5% gum, were...
Hydrogels based on triblock copolymers of polyethylene glycol and partially methacrylated poly[N-(2-hydroxypropyl) methacrylamide mono/dilactate] make up an attractive class biomaterials because their biodegradability, cytocompatibility, tunable thermoresponsive mechanical properties. If these properties are fine-tuned, the hydrogels can be three-dimensionally bioprinted, to generate, for instance, constructs cartilage repair. This study investigated whether polymer mentioned above with a...
Fine-tuning of bio-ink composition and material processing parameters is crucial for the development biomechanically relevant cartilage constructs.This study aims to design develop constructs with tunable internal architectures mechanical properties.More specifically, potential methacrylated hyaluronic acid (HAMA) added thermosensitive hydrogels composed poly[N-(2-hydroxypropyl)methacrylamide mono/dilactate] (pHPMAlac)/polyethylene glycol (PEG) triblock copolymers, optimize cartilage-like...
Three-dimensional (3D) bioprinting techniques can be used for the fabrication of personalized, regenerative constructs tissue repair. The current article provides insight into potential and opportunities 3D cartilage constructs. Although printing is already in orthopedic clinic, shift toward has not yet occurred. We believe that this will provide an important step forward field regeneration. allow incorporation cells biological cues during manufacturing process, to generate biologically...
Bioprinting is a promising tool to fabricate organized cartilage. This study aimed investigate the printability of gelatin-methacryloyl/gellan gum (gelMA/gellan) hydrogels with and without methacrylated hyaluronic acid (HAMA), explore (zone-specific) chondrogenesis chondrocytes, articular cartilage progenitor cells (ACPCs), multipotent mesenchymal stromal (MSCs) embedded in these bio-inks.The incorporating HAMA gelMA/gellan bio-ink increased filament stability, as measured using collapse...
Natural bone is a complex composite, consisting predominantly of collagen and hydroxyapatite (HA), which form highly organized, hierarchical structure from the nano- to macroscale. Because its biphasic, anisotropic, ultrafine structural design, tissue possesses excellent mechanical properties. Herein, inspired by composition microstructure natural bone, biphasic composite aligned strontium/copper-doped one-dimensional (Sr/Cu-doped 1D HA) poly(d,l-lactide) (PDLA) was developed. The presence...
The implantation of chondrocyte-laden hydrogels is a promising cartilage repair strategy. Chondrocytes can be spatially positioned in and thus defects, while current clinical cell therapies introduce chondrocytes the defect depth. main aim this study was to evaluate effect spatial chondrocyte distribution on reparative process. To reduce animal experiments, an ex vivo osteochondral plug model used evaluated. role delivered endogenous cells process investigated.Full thickness defects were...
Abstract Background Nucleus pulposus (NP) cell density is orchestrated by an interplay between nutrient supply and metabolite accumulation. Physiological loading essential for tissue homeostasis. However, dynamic also believed to increase metabolic activity could thereby interfere with regulation regenerative strategies. The aim of this study was determine whether reduce the NP interacting its energy metabolism. Methods Bovine explants were cultured in a novel bioreactor without milieus...
Low back pain is a global health problem that frequently caused by intervertebral disc degeneration (IVDD). Sulfated glycosaminoglycans (sGAGs) give the healthy nucleus pulposus (NP) high fixed charge density (FCD), which creates an osmotic pressure enables to withstand compressive forces. However, during IVDD sGAG reduction in NP compromises biomechanical function. The aim of this study was develop ex vivo explant model with reduced content and subsequently investigate restoration via...
In vitro evaluation of nucleus pulposus (NP) tissue regeneration would be useful, but current systems for NP culture are not ideal injections. The aim this study was to develop a long-term system that allows injections regenerative agents. Bovine caudal NPs were harvested and placed in the newly designed system. After equilibration 0.3 MPa volume fixed cultured 28 days. cell viability extracellular matrix composition remained unchanged during period gene expression profiles similar those...
Inflammatory cytokines play an important role in intervertebral disc degeneration. Although largely produced by immune cells, nucleus pulposus (NP) cells can also secrete them under various conditions, for example, free swelling. Thus, tissue hypotonicity may be inflammatory trigger NP cells. The aim of this study was to investigate whether decreased tonicity restricted swelling conditions (as occurring early degeneration) could initiate cascade that mediates further Healthy bovine balanced...
The implantation of chondrocyte-laden hydrogels is a promising cartilage repair strategy. Chondrocytes can be spa­tially positioned in and thus defects, while current clinical cell therapies introduce chondrocytes the defect depth. main aim this study was to evaluate effect spatial chondrocyte distribution on reparative process. To reduce animal experiments, an ex vivo osteochondral plug model used evaluated. role delivered endogenous cells process investigated.Full thickness defects...