A5029627944- Pancreatic function and diabetes
- Diabetes and associated disorders
- Growth Hormone and Insulin-like Growth Factors
- Diabetes Management and Research
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Meat and Animal Product Quality
- Birth, Development, and Health
- Sperm and Testicular Function
- Testicular diseases and treatments
- Cytokine Signaling Pathways and Interactions
- Adipose Tissue and Metabolism
- Diabetes Treatment and Management
- Renal and related cancers
- Fatty Acid Research and Health
- Pancreatitis Pathology and Treatment
- Sexual Differentiation and Disorders
- Milk Quality and Mastitis in Dairy Cows
- Pancreatic and Hepatic Oncology Research
- Neuroendocrine Tumor Research Advances
- MicroRNA in disease regulation
- Gestational Diabetes Research and Management
- Ruminant Nutrition and Digestive Physiology
- RNA modifications and cancer
- Probiotics and Fermented Foods
University of Copenhagen
2014-2025
Rigshospitalet
2010-2022
Copenhagen University Hospital
1989-2020
Aarhus University
2009-2014
Danish National Centre for Social Research
2014
Department of Medical Sciences
2013
Arla Foods (Denmark)
2011
Danish Cattle Research Centre
2006
Steno Diabetes Centers
1979-2001
Novo Nordisk (Denmark)
2001
Activated mononuclear cells appear to be important effector in autoimmune beta cell destruction leading insulin-dependent (type 1) diabetes mellitus. Conditioned medium from activated (from human blood) is cytotoxic isolated rat and islets of Langerhans. This activity was eliminated crude cytokine preparations by adsorption with immobilized, purified antibody interleukin-1 (IL-1). The islet-inhibitory the IL-1 (determined its comitogenic effect on thymocytes) were recovered acid wash....
The vitamin D receptor (VDR) is expressed in human testis, and (VD) has been suggested to affect survival function of mature spermatozoa. Indeed, VDR knockout mice VD deficient rats show decreased sperm counts low fertility. However, the cellular response complex, since it not solely dependent on expression, but also uptake circulating presence activity metabolizing enzymes. Expression enzymes previously investigated testis male reproductive tract. Therefore, we performed a comprehensive...
In this study we have investigated the role of suppressor cytokine signaling (SOCS) proteins in GH receptor-mediated signaling. GH-induced transcription was inhibited by SOCS-1 and SOCS-3, while SOCS-2 inducible SH2-containing protein (CIS) had no effect. By using chimeric SOCS it found that ability to inhibit GH-mediated located amino-terminal 40–80 amino acids. 46 acids C-terminal SH2 domain were required for inhibitory activity, a truncated having only 2 able transcription. Both SOCS-3...
Aims : NANOG is a key regulator of embryonic stem cell (ESC) self‐renewal and pluripotency. Our recent genome‐wide gene expression profiling study the precursor testicular germ tumours, carcinoma in situ testis (CIS), showed close similarity between ESC CIS, including high expression. In present we analysed protein during normal development human large series neoplastic/dysgenetic specimens. Methods results We detected abundant CIS CIS‐derived tumours with marked differences; seminoma...
The effect of glucagon-like peptide 1(7–36) amide [GLP-l(7–36) amide] on membrane potential, wholecell ATP-sensitive potassium channel (KATP) and Ca2+ currents, cytoplasmic concentration, exocytosis was explored in single human β-cells. GLP-1(7–36) induced depolarization that associated with inhibition whole-cell KATP current. In addition, GLP-l(7–36) (and forskolin) produced greater than fourfold potentiation Ca2+-dependent exocytosis. latter resulted part (40%) from acceleration influx...
Cytokines are soluble, antigen non‐specific, non‐immunoglobulin mediators produced and secreted by blood mononuclear cells interacting in the cellular immune‐response. To test possibility that cytokines participate autoimmune destruction of pancreatic beta‐cells leading to insulin‐dependent diabetes mellitus, isolated human or rat islets Langerhans were incubated for 7 days with cytokine‐rich, cell‐free supernatants from healthy donors stimulated without purified protein derivative...
The direct effects of human GH (hGH), ovine pituitary PRL (oPRL), and chorionic somatomammotropin [placental lactogen (hPL)] on the endocrine pancreas were studied in isolated pancreatic islets maintained tissue culture. Islets Langerhans by collagenase treatment obtained from adult NMRI mice or newborn Wistar rats. for up to 3 weeks petri dishes containing culture medium RPMI 1640 supplemented with calf serum normal serum. release insulin during islet content insulin, glucagon, DNA after...
The incretin hormones, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP), have been suggested to act as β-cell growth factors may therefore be of critical importance for the maintenance a proper mass. We investigated molecular mechanism incretin-induced replication in primary monolayer cultures newborn rat islet cells. GLP-1, GIP long-acting GLP-1 derivative, lira-glutide, increased 50–80% at 10–100 nM upon 24 h stimulus, whereas glucagon similar...
Suppressor of cytokine signaling 3 (SOCS-3) is a negative feedback regulator IFN-gamma signaling, shown up-regulated in mouse bone marrow cells by the proinflammatory cytokines interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and IFN-gamma. IL-1beta alone, or potentiated TNF-alpha, are cytotoxic to insulin producing pancreatic beta-cells beta-cell lines vitro suggested contribute specific destruction Type-1 diabetes mellitus (T1DM). Using doxycycline-inducible SOCS-3...
The susceptibility of a given muscle tissue to lipid oxidation may not only depend on the presence unsaturated fatty acids and balance between antioxidants prooxidants, but also composition skeletal muscle. In present study, effects dietary supplementation vitamin E (dl-α-tocopheryl acetate) copper in combination with high level monounsaturated were examined regard antioxidant concentration two muscles, longissimus (LD) psoas major (PM), representing different oxidative capacity. addition,...
Cyclosporin A (CyA) may induce clinical remission in newly diagnosed insulin-dependent diabetes mellitus patients. Recently, however, adverse effects of high doses CyA on rodent islets have been reported vivo and vitro. The possible direct the human endocrine pancreas were therefore evaluated. Islets isolated from eight necrokidney donors cultured presence a therapeutically relevant dose CyA, i.e., 100 ng/ml. During 5-day culture period release insulin was reduced by 36% (range 7-61%),...
The experimental protocols used in the investigation of stem cell–mediated liver regeneration rodents are characterized by activation hepatic cell compartment canals Hering followed transit amplification oval cells and their subsequent differentiation along lineages. Although numerous often interchangeably across species, a thorough comparative phenotypic analysis rats mice using well-established generally acknowledged molecular markers has not been provided. In present study, we evaluated...
The regulation of the growth pancreatic β-cell is poorly understood. There are previous indications a role GH in and insulin production islets. In present study we evidence for direct long-term effect on proliferation biosynthesis β-cells monolayer culture. culture medium RPMI 1640 supplemented with 2% normal human serum islets or dissociated islet cells from newborn rats maintained their insulin-producing capacity. When 1–1000 ng/ml pituitary recombinant attached, spread out, proliferated...
Abstract. The insulin-stimulated conversion of glucose to fatty acids (fatty acid synthesis) and the maximally norepinephrine-stimulated lipolysis were studied in isolated fat cells from normal male female rats, ovariectomized rats sexual hormone-treated rats. synthesis oscillated considerably more than This was found be due oestrous cycle, since high prooestrus low both oestrus dioestrus, while higher dioestrus. Oestradiol treatment testosterone for three days lowered increased lipolysis....
Significance In type 1 diabetes (T1D), the insulin-producing pancreatic β-cells are destroyed by immune system. Both genetic and environmental factors contribute to T1D risk. Candidate genes for identified genome-wide association studies have been proposed act at both system β-cell levels. This study shows that risk variant rs3825932 in candidate gene cathepsin H ( CTSH ) predicts function model systems human T1D. Collectively, our data indicate higher expression may protect against...