A5025369867- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Birth, Development, and Health
- Diabetes Treatment and Management
- Diet and metabolism studies
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Pharmacology and Obesity Treatment
- Receptor Mechanisms and Signaling
- Diabetes Management and Research
- Retinoids in leukemia and cellular processes
- Computational Drug Discovery Methods
- Muscle Physiology and Disorders
- Cholinesterase and Neurodegenerative Diseases
- Epigenetics and DNA Methylation
- Muscle metabolism and nutrition
- Congenital heart defects research
- Manufacturing Process and Optimization
- Circular RNAs in diseases
- Diabetes and associated disorders
- Sleep and related disorders
- Lymphatic System and Diseases
- Regulation of Appetite and Obesity
- Ion channel regulation and function
- Extracellular vesicles in disease
University of Copenhagen
2008-2024
Novo Nordisk (Denmark)
2020-2024
Steno Diabetes Centers
2008-2016
Rigshospitalet
2012-2016
Audi (Germany)
2016
Eppendorf (Germany)
1996
To investigate the effects of once-weekly subcutaneous (s.c.) semaglutide 2.4 mg on gastric emptying, appetite, and energy intake in adults with obesity.A double-blind, parallel-group trial was conducted 72 obesity, randomized to s.c. (dose-escalated mg) or placebo for 20 weeks. Gastric emptying assessed using paracetamol absorption following a standardized breakfast. Participant-reported appetite ratings Control Eating Questionnaire (CoEQ) responses were assessed, measured during ad libitum...
Physical inactivity is a risk factor for insulin resistance. We examined the effect of 9 days bed rest on basal and insulin-stimulated expression genes potentially involved in action by applying hypothesis-generating microarray parallel with candidate gene real-time PCR approaches 20 healthy young men. Furthermore, we investigated whether affected DNA methylation promoter region peroxisome proliferator-activated receptor-γ coactivator-1α (PPARGC1A) gene. Subjects were reexamined after 4 wk...
Monozygotic twins discordant for type 2 diabetes constitute an ideal model to study environmental contributions diabetic traits. We aimed examine whether global DNA methylation differences exist in major glucose metabolic tissues from these twins.
Glucagon/glucagon-like peptide-1 (GLP-1) receptor co-agonists may provide greater weight loss than agonists targeting the GLP-1 alone. We report results from three phase 1 trials investigating safety, tolerability, pharmacokinetics and pharmacodynamics of glucagon/GLP-1 co-agonist NNC9204-1177 (NN1177) for once-weekly subcutaneous use in adults with overweight or obesity. Our focus was a 12-week, multiple ascending dose (MAD), placebo-controlled, double-blind trial which (N=99) received...
Gene expression alterations have previously been associated with type 2 diabetes, however whether these changes are primary causes or secondary effects of diabetes is not known. As healthy first degree relatives people an increased risk developing they provide a good model in the search for disease.We determined gene profiles skeletal muscle biopsies from Caucasian males relatives, and controls. was measured using Affymetrix Human Genome U133 Plus 2.0 Arrays covering entire human genome....
Significance In type 1 diabetes (T1D), the insulin-producing pancreatic β-cells are destroyed by immune system. Both genetic and environmental factors contribute to T1D risk. Candidate genes for identified genome-wide association studies have been proposed act at both system β-cell levels. This study shows that risk variant rs3825932 in candidate gene cathepsin H ( CTSH ) predicts function model systems human T1D. Collectively, our data indicate higher expression may protect against...
Abstract Aims/hypothesis We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins. Methods measured miRNA levels monozygotic (MZ) twins discordant for using arrays. Further investigations selected miRNAs included target prediction, pathway analysis, silencing cells association analyses a separate cohort 164 non-diabetic MZ dizygotic The effects elevated glucose insulin...
OBJECTIVE—Retinol-binding protein (RBP) 4 is an adipokine of which plasma levels are elevated in obesity and type 2 diabetes. The aims the study were to identify determinants RBP4 mRNA expression subcutaneous adipose tissue (SAT) skeletal muscle investigate association between vivo measures glucose metabolism. RESEARCH DESIGN AND METHODS—The population included 298 elderly twins (aged 62–83 years), with tolerance ranging from normal overt diabetes, 178 young 25–32 years) 58–66 nondiabetic...
Subjects with a low birth weight (LBW) display increased risk of developing type 2 diabetes (T2D). We hypothesized that this is associated defects in muscle adaptations following acute and regular physical activity, evident by impairments the exercise-induced activation AMPK signaling. investigated 21 LBW normal (NBW) subjects during 1 h exercise performed at same relative workload before after 12 wk training. Multiple skeletal biopsies were obtained exercise. Protein levels phosphorylation...
The protein complex AMP-activated kinase (AMPK) is believed to play an important role in the regulation of skeletal muscle glucose and lipid metabolism. Defects AMPK system might therefore be factor pathogenesis type 2 diabetes. We aimed identify genetic environmental mechanisms involved expression activity examine association between levels on one hand, fat metabolism other. investigated biopsies from 100 young 82 older mono- dizygotic nondiabetic twins excised during basal...
Carboxylesterase 1 (CES1) appears to play an important role in the control of metabolism triglycerides and cholesterol adipocytes other cell types including hepatocytes. Therefore, it is relevant gain insights into genetic versus non-genetic mechanisms involved CES1 mRNA expression. Here, we investigated expression level adipose tissue its association with measures adiposity metabolic function a population elderly twins. Furthermore, heritability effect gene duplication were assessed.A total...
Abstract Glucagon/glucagon-like peptide-1 (GLP-1) receptor co-agonists may provide greater weight loss than agonists targeting the GLP-1 alone. We report results from three phase 1 trials investigating glucagon/GLP-1 co-agonist NNC9204-1177 (NN1177) for once-weekly subcutaneous use in adults with overweight or obesity. Our focus was a 12–week multiple ascending dose (MAD), placebo-controlled, double-blind trial which (N=99) received NN1177 (dose-escalated to treatment doses of 200, 600,...
Type 2 diabetes is characterized by reduced muscle glycogen synthesis. The key enzyme in this process, synthase (GS), activated via proximal insulin signaling, but the exact molecular events remain unknown. Previously, we demonstrated that phosphorylation of Thr 308 on Akt (p-Akt-Thr ), Akt2 activity, and GS activity were positively associated with sensitivity. Here, same study population, determined influence several upstream elements canonical PI3K signaling activation. One-hundred...
NN1177 is a glucagon/glucagon-like peptide 1 receptor coagonist investigated for chronic weight management and treatment of nonalcoholic steatohepatitis. Here, we show concentration-dependent downregulation cytochrome P450 (P450) enzymes using freshly isolated human hepatocytes treated with this linear 29-amino acid peptide. Notably, reductions in CYP3A4 mRNA expression (57.2%–71.7%) activity (18.5%–51.5%) were observed clinically relevant concentration 100 nM NN1177. CYP1A2 CYP2B6 also...
Regular exercise can reduce incidence and progression of breast cancer, but the mechanisms for such effects are not fully understood.
Developmental programming of human muscle stem cells could in part explain why individuals born with low birth weight (LBW) have an increased risk developing type 2 diabetes (T2D) later life. We hypothesized that immature cell functions including abnormal differentiation potential and metabolic function link LBW the T2D. Design/Settings/Participants: recruited 23 young men 16 age-matched control subjects normal weight. Biopsies were obtained from vastus lateralis, isolated cultured into...