Paige Haas
A5072729152- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- vaccines and immunoinformatics approaches
- Complement system in diseases
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Malaria Research and Control
- Trypanosoma species research and implications
- Advanced Proteomics Techniques and Applications
- COVID-19 Clinical Research Studies
- Lysosomal Storage Disorders Research
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
Gladstone Institutes
2017-2024
University of California, San Francisco
2017-2024
Quantitative BioSciences
2017-2024
Infectious Disease Research Institute
2016-2022
Center for Infectious Disease Research
2016-2021
A newly described coronavirus named severe acute respiratory syndrome 2 (SARS-CoV-2), which is the causative agent of disease 2019 (COVID-19), has infected over 2.3 million people, led to death more than 160,000 individuals and caused worldwide social economic disruption1,2. There are no antiviral drugs with proven clinical efficacy for treatment COVID-19, nor there any vaccines that prevent infection SARS-CoV-2, efforts develop hampered by limited knowledge molecular details how SARS-CoV-2...
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 closely related more lethal but less transmissible coronaviruses SARS-CoV-1 Middle East (MERS-CoV). Here, we have carried out comparative viral-human protein-protein interaction viral protein localization analyses for all three viruses. Subsequent functional genetic screening identified host factors that functionally impinge on...
ABSTRACT An outbreak of the novel coronavirus SARS-CoV-2, causative agent COVID-19 respiratory disease, has infected over 290,000 people since end 2019, killed 12,000, and caused worldwide social economic disruption 1,2 . There are currently no antiviral drugs with proven efficacy nor there vaccines for its prevention. Unfortunately, scientific community little knowledge molecular details SARS-CoV-2 infection. To illuminate this, we cloned, tagged expressed 26 29 viral proteins in human...
Human Immunodeficiency Virus (HIV) relies on host molecular machinery for replication. Systematic attempts to genetically or biochemically define these factors have yielded hundreds of candidates, but few been functionally validated in primary cells. Here, we target 426 genes previously implicated the HIV lifecycle through protein interaction studies CRISPR-Cas9-mediated knock-out human CD4+ T cells order systematically assess their functional roles We achieve efficient knockout (>50%...
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19), was declared a pandemic infection in March 2020. As December 2020, two COVID-19 vaccines have been authorized for emergency use by U.S. Food and Drug Administration, but there are no effective drugs to treat COVID-19, mitigation efforts like physical distancing had social economic consequences. In this perspective, we discuss how proteomic research community can leverage...
Background: In a phase 3 trial in African infants and children, the RTS,S/AS01 vaccine (GSK) showed moderate efficacy against clinical malaria. We sought to further understand RTS,S/AS01-induced immune responses associated with protection. Methods: Applying blood transcriptional module (BTM) framework, we characterized transcriptomic response vaccination antigen-stimulated (and vehicle control) peripheral mononuclear cells sampled from subset of participants at baseline month (1-month...
During HIV infection of CD4+ T cells, ubiquitin pathways are essential to viral replication and host innate immune response; however, the role specific E3 ligases is not well understood. Proteomics analyses identified 116 single-subunit expressed in activated primary human cells. Using a CRISPR-based arrayed spreading infectivity assay, we systematically knocked out E3s from cells infected them with NL4-3 GFP reporter HIV-1. We found 10 significantly positively or negatively affected...
ABSTRACT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We recently adapted this technology primary T cells generate a high-throughput platform analyzing role host factors in pathogen infection and lifecycle. Here, we describe applications system investigate HIV pathogenesis CD4+ cells. Briefly, ribonucleoproteins (crRNPs) are synthesized vitro delivered activated by...
Abstract In a phase 3 trial in African infants/children, the RTS,S/AS01 (GSK) vaccine showed moderate efficacy against clinical malaria. We aimed to identify RTS,S/AS01-induced signatures associated with malaria by analyzing antigen-stimulated peripheral blood mononuclear cells sampled from subset of participants at baseline and month (one post-third dose). vaccination was downregulation B-cell monocyte-related transcriptional modules (BTMs) upregulation T-cell related BTMs, as well higher...