A5101977588- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Plant Physiology and Cultivation Studies
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Fungal and yeast genetics research
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Arsenic contamination and mitigation
- Alcohol Consumption and Health Effects
- Aluminum toxicity and tolerance in plants and animals
- Trace Elements in Health
- Plant tissue culture and regeneration
- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Animal Behavior and Reproduction
- Plant pathogens and resistance mechanisms
- Cassava research and cyanide
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Constructed Wetlands for Wastewater Treatment
Hefei Normal University
2020-2024
Sichuan Agricultural University
2019-2024
Baoji City Central Hospital
2014-2022
Ningbo Academy of Agricultural Sciences
2012-2022
Northeast Agricultural University
2022
Rice Research Institute
2019-2021
Fudan University
2004-2011
University of California, Berkeley
1999-2000
Abstract To investigate rate-limiting factors for glutathione and phytochelatin (PC) production the importance of these compounds heavy metal tolerance, Indian mustard (Brassica juncea) was genetically engineered to overexpress Escherichia coli gshI gene encoding γ-glutamylcysteine synthetase (γ-ECS), targeted plastids. The γ-ECS transgenic seedlings showed increased tolerance Cd had higher concentrations PCs, γ-GluCys, glutathione, total non-protein thiols compared with wild-type (WT)...
Abstract Wetland plants are being used successfully for the phytoremediation of trace elements in natural and constructed wetlands. This study demonstrates potential water hyacinth ( Eichhornia crassipes ), an aquatic floating plant, six elements. The ability to take up translocate elements—As(V), Cd(II), Cr(VI), Cu(II), Ni(II), Se(VI)—was studied under controlled conditions. Water accumulated Cd Cr best, Se Cu at moderate levels, was a poor accumulator As Ni. highest levels found shoots...
Abstract An important pathway by which plants detoxify heavy metals is through sequestration with heavy-metal-binding peptides called phytochelatins or their precursor, glutathione. To identify limiting factors for heavy-metal accumulation and tolerance, to develop transgenic an increased capacity accumulate and/or tolerate metals, the Escherichia coli gshII gene encoding glutathione synthetase (GS) was overexpressed in cytosol of Indian mustard (Brassica juncea). The GS accumulated...
Summary mi RNA s contribute to plant resistance against pathogens. Previously, we found that the function of miR398b in immunity rice differs from Arabidopsis. However, underlying mechanisms are unclear. In this study, characterized mutants target genes and demonstrated multiple superoxide dismutase miR398b‐regulated blast fungus Magnaporthe oryzae . Out four miR398b, mutations Cu/Zn‐Superoxidase Dismutase1 ( CSD 1 ), 2 Os11g09780 Superoxide DismutaseX , SODX ) led enhanced M. increased...
Brassinosteroids (BRs) play an essential role in plant growth and development, have been implicated many physiological responses. However, little is known about the of BRs response to oxidative stress. In this study, we identified a novel insertion allele ( det2‐9 ) DET2 gene Arabidopsis based on molecular, genetic approaches. We found that det2 mutant exhibited enhanced resistance The stress plants was correlated with constitutive increase superoxide dismutase (SOD) activity increased...
Fitness cost is a common phenomenon in rice blast disease-resistance breeding. MiR396 highly conserved microRNA (miRNA) family targeting Growth Regulating Factor (OsGRF) genes. Mutation at the target site of miR396 certain OsGRF gene or blocking expression leads to increased grain yield. Here we demonstrated that fitness can be trade-off miR396-OsGRFs module via balancing growth and immunity against fungus. The accumulation isoforms was significantly susceptible accession, but fluctuated...
Abstract MicroRNAs (miRNAs) play important roles in rice response to Magnaporthe oryzae , the causative agent of blast disease. Studying miRNAs is great significance for disease control. Osa‐miR167d belongs a conserved miRNA family targeting auxin responsive factor ( ARF ) genes that act developmental and stress‐induced responses. Here, we show plays negative role immunity against M. by suppressing its target gene. The expression was significantly suppressed resistant accession at after 24 h...
Reactive oxygen species (ROS) act as a group of signaling molecules in rice functioning regulation development and stress responses. Respiratory burst oxidase homologues (Rbohs) are key enzymes generation ROS. However, the role nine Rboh family members was not fully understood multiple disease resistance yield traits. In this study, we constructed mutants each genes detected their requirement Our results revealed that mutations five (RbohA, RbohB, RbohE, RbohH, RbohI) lead to compromised...
Gastric cancer is one of the most common forms malignant tumor, and development anti‑gastric drugs with minimal toxicity clinical importance. Allicin extracted from Allium sativum (garlic). Recent research, including experiments, has shown that garlic anticancer tumor suppressive effects. The present study aimed to investigate effects allicin on MGC‑803 human gastric carcinoma cell line, further explore possible mechanisms its suppressor cells were initially examined using an...
Abstract MicroRNAs (miRNAs) are known to fine‐tune growth, development, and stress‐induced responses. Osa‐miR1873 is a rice‐specific miRNA targeting LOC_Os05g01790 . Here, we show that fine‐tunes rice immunity against Magnaporthe oryzae yield traits via was significantly upregulated in susceptible accession but downregulated resistance at 24 h post‐inoculation (hpi) of M. Overexpressing enhanced susceptibility M compromised induction defense In contrast, blocking through target mimicry...
Grain formation is fundamental for crop yield but vulnerable to abiotic and biotic stresses. Rice grain production threatened by the false smut fungus Ustilaginoidea virens, which specifically infects rice floral organs, disrupting fertilization seed formation. However, little known about molecular mechanisms of U. virens–rice interaction genetic basis resistance. Here, we report that virens secretes a cytoplasmic effector, UvCBP1, facilitate infection flowers. Mechanistically, UvCBP1...
Abstract The flower-infecting fungus Ustilaginoidea virens causes rice false smut, which is a severe emerging disease threatening (Oryza sativa) production worldwide. False smut not only reduces yield, but more importantly produces toxins on grains, posing great threat to food safety. U. invades spikelets via the gap between 2 bracts (lemma and palea) enclosing floret specifically infects stamen pistil. Molecular mechanisms for virens–rice interaction are largely unknown. Here, we...
To determine the importance of glutathione reductase (GR, EC 1.6.4.2) for heavy metal accumulation and tolerance, a bacterial GR was expressed in Indian mustard ( Brassica juncea L.), targeted to cytosol or plastids. activity cytosolic transgenics (cytGR) about two times higher compared wild‐type plants; plastidic (cpGR) up 50 higher. When treated with 100 μ M CdSO 4 , cytGR plants did not differ from wild type cadmium tolerance accumulation. CpGR plants, however, showed enhanced at...
MicroRNAs (miRNAs) play essential roles in rice immunity against Magnaporthe oryzae, the causative agent of blast disease. Here we demonstrate that Osa-miR162a fine-tunes M. oryzae and yield traits. Overexpression enhances resistance to accompanying enhanced induction defense-related genes accumulation hydrogen peroxide (H2O2). In contrast, blocking Osa-miR162 by overexpressing a target mimic susceptibility fungus associating with compromised gene expression H2O2 accumulation. Moreover,...
Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases rice. An increasing number microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. and coordinate with growth development.
Background The giant panda has an interesting bamboo diet unlike the other species in order of Carnivora. umami taste receptor gene T1R1 been identified as a pseudogene during its genome sequencing project and confirmed using different sample. estimated mutation time for this is about 4.2 Myr. Such coincided with panda's dietary change also reinforced herbivorous life style. However, preserved herbivores such cow horse, we need to look reasons behind switch. Methodology/Principal Findings...
MicroRNAs (miRNAs) play vital roles in plant development and defence responses against various stresses. Here, we show that blocking miR1871 improves rice resistance Magnaporthe oryzae enhances grain yield simultaneously. The transgenic lines overexpressing (OX1871) exhibit compromised resistance, suppressed reduced panicle number resulting slightly decreased yield. In contrast, the (MIM1871) improved enhanced significantly increased leading to per plant. RNA-seq assay response assays reveal...
microRNAs act as fine-tuners in the regulation of plant growth and resistance against biotic abiotic stress. Here we demonstrate that rice miR1432 fine-tunes yield blast disease via different modules. Overexpression leads to compromised decreased yield, whereas blocking using a target mimic results enhanced yield. suppresses expression LOC_Os03g59790, which encodes an EF-hand family protein 1 (OsEFH1). OsEFH1 but grain Further study revealed are differentially responsive chitin,...
Rice production is threatened by multiple pathogens. Breeding cultivars with broad-spectrum disease resistance necessary to maintain and improve crop production. Previously we found that overexpression of miR160a enhanced rice blast resistance. However, it unclear whether also regulates against other pathogens, what the downstream signaling pathways are. Here, demonstrate positively causative agents blast, leaf blight sheath in rice. Mutations miR160a-targeted Auxin Response Factors result...
MicroRNAs (miRNAs) act as regulators of plant development and multiple stress responses. Here we demonstrate that the rice miR171b-SCL6-IIs module regulates balance between blast resistance, grain yield, flowering. miR171b-overexpressing plants (OX171b) displayed increased resistance accompanied with enhanced defense responses late heading, whereas blocking miR171b expression in (MIM171) led to greater susceptibility disease, associated compromised early heading. Either overexpressing or...
Arabidopsis RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) is an important tool for engineering broad-spectrum disease resistance against multiple pathogens. Ectopic expression of RPW8.1 leads to enhanced with cell death at leaves and compromised plant growth, implying a regulatory mechanism balancing RPW8.1-mediated growth. Here, we show that constitutively enhances the transcription factor WRKY51 activates salicylic acid ethylene signalling pathways; in turn suppresses expression, forming...