A5040540628- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Cardiac electrophysiology and arrhythmias
- Signaling Pathways in Disease
- Cardiac Fibrosis and Remodeling
- Advanced Proteomics Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Congenital heart defects research
- ATP Synthase and ATPases Research
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Pain Mechanisms and Treatments
- Advanced biosensing and bioanalysis techniques
- Nicotinic Acetylcholine Receptors Study
- Redox biology and oxidative stress
- Sleep and Wakefulness Research
- COVID-19 Clinical Research Studies
- Cardiomyopathy and Myosin Studies
- Genetics, Aging, and Longevity in Model Organisms
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- Venomous Animal Envenomation and Studies
Institute of Biomedical Sciences, Academia Sinica
2013-2024
Academia Sinica
2004-2005
National Defense Medical Center
2004
National Defense University
2004
Genomics Research Center, Academia Sinica
2004
Scripps Research Institute
2004
Tri-Service General Hospital
2004
We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four SARS in different time courses, a total of 38 differential spots were selected for protein identification. Most proteins identified are phase proteins, their presence represents consequence serial cascades initiated SARS-coronavirus infection. There several that never been before electrophoresis, among...
Pressure-overload stress to the heart causes pathological cardiac hypertrophy, which increases risk of morbidity and mortality. However, detailed signaling pathways induced by pressure overload remain unclear. Here we used phosphoproteomics delineate in myocardium responding acute chronic hypertrophy mice. Myocardial samples at 4 time points (10, 30, 60 min 2 weeks) after transverse aortic banding (TAB) mice underwent quantitative assay. Temporal profiles showed 360 phosphorylation sites...
In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy combines tumor xenotransplantation model in nude with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401) derived stomach, nasopharyngeal, colon, oral and brain cancers were subcutaneously inoculated into compared control injected phosphate-buffered saline. One month later, cells was...
Carbohydrate–lectin recognition plays important roles in cell–cell communication, proliferation, and differentiation. We report here a new approach of using carbohydrate-encapsulated gold nanoparticle (shown purple) as an affinity probe for the efficient separation enrichment target proteins, then protein identification epitope mapping by MALDI-TOF MS. Supporting information this article is available on WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z500023_s.pdf or from author....
Loss of the mitochondrial fission enzyme dynamin-related protein 1 (Drp1) in cardiomyocytes results energy shortage and heart failure. We aim to understand intracellular signal pathway extracellular factors regulating Drp1 phosphorylation morphology function cardiomyocytes. found cyclic mechanical stretching induced through focal adhesion kinase (FAK) neonatal rat ventricular myocytes (NRVMs). FAK regulated levels. Extracellular fibronectin activated caused signal-regulated 1/2 (ERK1/2)....
Abstract Hyperalgesic priming, a form of pain plasticity initiated by initial injury, leads to heightened sensitivity subsequent noxious stimuli, contributing chronic development in animals. While astrocytes play active roles modulating synaptic transmission various models, their specific involvement hyperalgesic priming remains elusive. Here, we show that spinal are essential for formation mouse model acid-induced muscle pain. We observed astrocyte activation 4 h after acid injection, and...
Pathological cardiac hypertrophy, a dynamic remodeling process, is major risk factor for heart failure. Although number of key regulators and related genes have been identified, how the transcription factors (TFs) dynamically regulate associated control morphological electrophysiological changes during hypertrophic process are still largely unknown. In this study, we obtained time-course transcriptomes at five time points in four weeks from male murine hearts subjected to transverse aorta...
The Cav3.2 T-type calcium channel is implicated in various pathological conditions, including cardiac hypertrophy, epilepsy, autism, and chronic pain. Phosphorylation of by multiple kinases plays a pivotal role regulating its function. calcium/calmodulin-dependent serine/threonine phosphatase, calcineurin, interacts physically with modulates activity. However, it remains unclear whether calcineurin dephosphorylates Cav3.2, the specific spatial regions on involved, extent quantitative impact....
Inhibition of T-type calcium channels (CaV3) prevents development diseases related to cardiovascular and nerve systems. Further, knockout animal studies have revealed that some are mediated by specific subtypes CaV3. However, subtype-specific CaV3 inhibitors for therapeutic purposes or studying the physiological roles missing. To bridge this gap, we employed our spider venom library uncovered Avicularia spec. ("Amazonas Purple", Peru) tarantula inhibited CaV channel subtypes. By using...
The Cav3.2 T-type calcium channel is implicated in various pathological conditions, including cardiac hypertrophy, epilepsy, autism, and chronic pain. Phosphorylation of by multiple kinases plays a pivotal role regulating its function. calcium/calmodulin-dependent serine/threonine phosphatase, calcineurin, interacts physically with modulates activity. However, it remains unclear whether calcineurin dephosphorylates Cav3.2, the specific spatial regions on involved, extent quantitative impact....
The Cav3.2 T-type calcium channel is implicated in various pathological conditions, including cardiac hypertrophy, epilepsy, autism, and chronic pain. Phosphorylation of by multiple kinases plays a pivotal role regulating its function. calcium/calmodulin-dependent serine/threonine phosphatase, calcineurin, interacts physically with modulates activity. However, it remains unclear whether calcineurin dephosphorylates Cav3.2, the specific spatial regions on involved, extent quantitative impact....