A5025547227- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Diabetes and associated disorders
- Neurogenetic and Muscular Disorders Research
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Blood properties and coagulation
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Regional Development and Management Studies
- Chemokine receptors and signaling
- Cruise Tourism Development and Management
University of Virginia
2021-2025
University of Connecticut
2013-2020
Glial cells that express the NG2 proteoglycan and α receptor for PDGF (NG2 cells, polydendrocytes) make up fifth major cell population serves as oligodendrocyte progenitor in postnatal CNS. Although recent studies have suggested differences their proliferation differentiation gray white matter, mechanism underlying observed has been unclear. Using organotypic slice cultures from forebrain cerebellum of early NG2creBAC:ZEG mice, we compared basal growth factor-induced matter. matter exhibited...
Transfusion-induced anti-red blood cell (RBC) alloantibodies pose a significant risk to patients who require chronic transfusions. Anti-RBC can be remarkably short-lived (i.e. evanescent), leading clinically relevant that are not detected in later pre-transfusion antibody screens. Subsequent transfusion of alloantigen-positive RBCs stimulates rapid memory response may induce delayed hemolytic reaction (DHTR), causing morbidity and occasional mortality chronically transfused patients. It is...
BACKGROUND: Alloimmunization to transfused red blood cells (RBCs) remains a significant clinical problem. However, the that initiate immune responses RBCs remain incompletely characterized. Recently published work has identified splenic marginal zone B (MZB) as being critically required for production of anti-RBC alloantibodies in response RBCs. In infectious models, MZB cell activation been shown depend on unique population macrophages (MZMs). We hypothesized MZMs would capture stored and...
NG2 expressing cells (polydendrocytes, oligodendrocyte precursor cells) are the fourth major glial cell population in central nervous system. During embryonic and postnatal development they actively proliferate generate myelinating oligodendrocytes. These have commonly been studied primary dissociated cultures, neuron cocultures, fixed tissue. Using newly available transgenic mouse lines slice culture systems can be used to investigate proliferation differentiation of lineage both gray white...
Abstract Background Despite the significant adverse clinical consequences of RBC alloimmunization, our understanding signals that induce immune responses to transfused RBCs remains incomplete. Though storage has been shown enhance alloimmunization in hen egg lysozyme, ovalbumin, and human Duffy (HOD) alloantigen mouse model, molecular leading activation this system remain unclear. Given nonclassical major histocompatibility complex (MHC) Class I molecule CD1D can bind multiple different...
Abstract Oligodendrocyte precursor cells (OPCs), also known as NG2 or polydendrocytes, are distributed widely throughout the developing and mature central nervous system. They remain proliferative life an important source of myelinating in normal demyelinating brain well a glioma, most common type primary tumor with poor prognosis. OPC proliferation is dependent on signaling mediated by platelet‐derived growth factor (PDGF) AA binding to its alpha receptor (PDGFRα). Here, we describe group...
Studies of human patients have shown that most anti-RBC alloantibodies are IgG1 or IgG3 subclasses, although it is unclear why transfused RBCs preferentially drive these subclasses over others. Though mouse models allow for the mechanistic exploration class-switching, previous studies RBC alloimmunization in mice focused more on total IgG response than relative distribution, abundance, mechanism subclass generation. Given this major gap, we compared distribution generated to protein alum...
Studies of human patients have shown that most anti-RBC alloantibodies are IgG1 or IgG3 subclasses, though it is unclear why transfused RBCs preferentially drive these subclasses over others. Though mouse models allow for the mechanistic exploration class-switching, previous studies RBC alloimmunization in mice focused more on total IgG response than relative distribution, abundance, mechanism subclass generation. Given this major gap, we compared distribution generated to protein alum...
Current methods of measuring anti-red blood cell (RBC) antibody levels are limited in their ability to provide absolute quantification. This is due inherent differences the affinity for RBC antigens among primary antisera as well IgG isotypes secondary reagents. Given important biological effector function observed between different subclasses, we set out develop a research-based flow cytometry protocol that would quantification subclass-specific bound RBCs. We took advantage recent...