A5027154287- Viral-associated cancers and disorders
- Iron Metabolism and Disorders
- Cytomegalovirus and herpesvirus research
- Soil Carbon and Nitrogen Dynamics
- Parvovirus B19 Infection Studies
- Legume Nitrogen Fixing Symbiosis
- Lymphoma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Enzyme-mediated dye degradation
- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- T-cell and Retrovirus Studies
- Microbial Metabolism and Applications
- Microbial Fuel Cells and Bioremediation
- Histiocytic Disorders and Treatments
- Microbial Metabolites in Food Biotechnology
- Advanced Chemical Sensor Technologies
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Nuclear Structure and Function
- Planetary Science and Exploration
- Herpesvirus Infections and Treatments
- Polysaccharides and Plant Cell Walls
- Genomics and Chromatin Dynamics
Università degli Studi della Tuscia
2010-2021
Viterbo University
2019-2020
Sapienza University of Rome
2002-2017
Natura (Brazil)
2012
AgroBio
2011
University of Amsterdam
1988-1992
National Institutes of Health
1984-1986
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1985-1986
Health and Human Development (2HD) Research Network
1986
Institute of Art
1986
The application of beneficial microorganisms at transplanting can promote rapid transplant establishment (starter effect) for achieving early and high yields. aim this study was to evaluate the biostimulant effects Glomus intraradices BEG72 (G) Trichoderma atroviride MUCL 45632 (T) alone or in combination on plant growth parameters, yield, chlorophyll index (SPAD), fluorescence mineral composition several vegetable crops.
Treatment of K562 cells with the iron chelator desferrioxamine results in gradual increase total cell receptors for transferrin. Receptor number rises 2.5-4.5-fold over 24 h and remains at elevated level if is continuously present. Preincubation ferric chloride abolishes effect. The drug has no effect on 7-h half-life receptor. increased can be accounted by a specific rate receptor biosynthesis which reaches 3-4 times that seen untreated 6 after addition chelator. Isolation mRNA from treated...
Chitin synthetase, in the zymogen form, was extracted with digitonin from a particulate fraction Saccharomyces cerevisiae and converted into active form by treatment immobilized trypsin. When activated enzyme incubated UDP-GlcNAc other components of an assay mixture, chitin precipitate formed, trapping large portion synthetase. The easily frm gel recovery approximately 50% enrichment 100-fold. Further purification obtained repeating step. After polyacrylamide electrophoresis presence sodium...
A number of strains Candida albicans were tested for germ tube formation after induction by N-acetyl-D-glucosamine (GlcNAc) and other simple (proline, glucose plus glutamine) or complex (serum) compounds. proportion (high responders) induced to form tubes evolving true hyphae GlcNAc alone proline glutamine mixture. The majority low responders because they could be only serum GlcNAc-serum medium. Two found nonresponders: grew as pseudohyphae in serum. Despite minor quantitative differences,...
Treatment of K562 cells, a human erythroleukemia cell line, with desferrioxamine raised the levels receptor for transferrin (Tf) two- to threefold over that control cells. The were reduced by at least 50 and 35% in cells treated diferric Tf ferric ammonium citrate, respectively. These changes total cellular receptors no alteration proportion found on surface. half-lives identical desferrioxamine, Tf, or citrate. Cells metabolically labeled [35S]methionine showed 2.5-fold increase rate...
Summary: Blastospores of Candida albicans were readily agglutinated by Concanavalin A (Con A) owing to the specific binding this lectin mannan receptors cell surface. When was extracted from wall neutral buffers, alkali and acid, agglutination decreased or lost depending on degree extraction. relatively mild treatment sufficient derange multilayered organization transform it into a uniform, medium-density structure having about same thickness as untreated wall. After more drastic extraction,...
The aim of the present work is to develop nanoemulsions (NEs), nanosized emulsions, manufactured for improving delivery active pharmaceutical ingredients. In particular, composed Neem seed oil, contain rich bioactive components, and Tween 20 as nonionic surfactant were prepared. A mean droplet size ranging from 10 100 nm was obtained by modulating oil/surfactant ratio. Physicochemical characterisation carried out evaluating size, ζ-potential, microviscosity, polarity turbidity external shell...
We have examined the mechanism by which hemin regulates expression of human transferrin receptor.Previous work led to suggestion that regulatory signal is provided heme (Ward J. H.,
Summary: Yeast cells of Candida albicans growing at 25 °C in glucose/yeast extract medium cannot germinate by dilution into fresh 37 before reaching the stationary phase growth. However, any stage growth are able to form germ-tubes giving true hyphae when incubated presence inducers such as serum or N-acetyl-d-glucosamine. Thus, not endowed with a specific ‘competence’ germinate.
Autophagy, an important degradation system involved in maintaining cellular homeostasis, serves also to eliminate pathogens and process their fragments for presentation the immune system. Several viruses have been shown interact with host autophagic machinery suppress or make use of this catabolic pathway enhance survival replication. Epstein Barr virus (EBV) is a γ-herpes associated number malignancies epithelial lymphoid origin which establishes predominantly latent infection. Latent EBV...
Analysing the dependence of bacteria adhesion on diameter nanofibres to improve cell-to-material for target applications.
Following a pulse with 59Fe-transferrin, K562 erythroleukemia cells incorporate significant amount of 59Fe into ferritin. Conditions or manipulations which alter the supply iron to result in changes rate ferritin biosynthesis consequent variations size pool. Overnight exposure donors such as diferric transferrin hemin increases level 2-4- 6-8-fold above that control, respectively. Treatment anti-human receptor antibody, OKT9 (which reduces uptake by decreasing number receptors) lowers...
Abstract Resveratrol (3,4′,5-trihydroxy-trans-stilbene), a polyphenolic natural product, shows chemopreventive properties against several cancers, heart diseases, inflammation, and viral infections. Epstein Barr virus (EBV), γ-herpesvirus, contributes to the development of human cancers including Burkitt's lymphoma (BL). In this study, we asked whether treatment with resveratrol would affect viability EBV-positive BL cells displaying different forms latency. We report here that resveratrol,...
We have investigated the mechanisms involved in regulation of ferritin biosynthesis K562 human erythroleukemia cells during prolonged exposure to iron. show that, upon addition hemin (an efficient iron donor) cell culture, rate reaches a maximum after few hours and then decreases. During 24-hr incubation with donor concentrations total heavy (H) light (L) subunit mRNAs rise 2- 5-fold 3-fold, respectively, over control values, while amount protein increases 10- 30-fold. The hemin-induced...