A5082586077- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Aquaculture disease management and microbiota
- Neonatal Respiratory Health Research
- Antibiotic Resistance in Bacteria
- Dermatology and Skin Diseases
- Pediatric health and respiratory diseases
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Metabolomics and Mass Spectrometry Studies
- Bacterial Genetics and Biotechnology
- Sepsis Diagnosis and Treatment
- Aquaculture Nutrition and Growth
- IL-33, ST2, and ILC Pathways
- Respiratory Support and Mechanisms
- Respiratory viral infections research
- Immune Response and Inflammation
- Liver Diseases and Immunity
- Immunodeficiency and Autoimmune Disorders
- Cystic Fibrosis Research Advances
- Fibroblast Growth Factor Research
- Nosocomial Infections in ICU
- Neonatal Health and Biochemistry
- Gut microbiota and health
- Respiratory and Cough-Related Research
Third Affiliated Hospital of Sun Yat-sen University
2016-2025
Sun Yat-sen University
2016-2025
Bridge University
2023
University of Macau
2023
Sun Yat-sen Memorial Hospital
2023
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2021-2022
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
2021-2022
National University Health System
2018
National University of Singapore
2018
Serum resistance is a poorly understood but common trait of some difficult-to-treat pathogenic strains bacteria. Here, we report that glycine, serine and threonine catabolic pathway down-regulated in serum-resistant Escherichia coli, whereas exogenous glycine reverts the serum effectively potentiates to eliminate clinically-relevant bacterial pathogens vitro vivo. We find increases formation membrane attack complex on through two previously unrecognized regulations: 1) negatively positively...
Multidrug-resistant Escherichia coli metabolomes are reprogrammed by glutamine to be sensitive ampicillin in vitro and vivo.
Chronic allergic asthma is characterized by Th2-typed inflammation, and contributes to airway remodeling the deterioration of lung function. However, initiating factor that links inflammation unknown. Thymic stromal lymphopoietin (TSLP), an epithelium-derived cytokine, can strongly activate dendritic cells (DCs) through TSLP-TSLPR OX40L-OX40 signaling pathways promote Th2 differentiation. To determine whether TSLP underlying trigger in chronic allergen-induced asthma, we induced mice...
Macrophages restrict bacterial infection partly by stimulating phagocytosis and release of cytokines complement components. Here, we treat macrophages with LPS a pathogen, demonstrate that expression cytokine IL-1β increase to transient peak 8 12 h post-treatment, while component 3 (C3) continues rise for 24 post-treatment. Metabolomic analysis suggests correlation between the cellular concentrations succinate inosine C3. This may involve regulatory feedback mechanism, whereby stimulates...
Pathogenic strains of bacteria are often highly adept at evading serum-induced cell death, which is an essential complement-mediated component the innate immune response. This phenomenon, known as serum-resistance, poorly understood, and a result, no effective clinical tools available to restore serum-sensitivity pathogenic bacteria. Here, we provide evidence that exogenous glycine reverses defects in glycine, serine threonine metabolism associated with serum resistance, restores...
Development of low-cost and eco-friendly approaches to fight bacterial pathogens is especially needed in aquaculture. We previously showed that exogenous malate reprograms zebrafish's metabolome potentiate zebrafish survival against Vibrio alginolyticus infection. However, the underlying mechanism unknown. Here, we use GC-MS based metabolomics identify malate-triggered metabolic shift. An activated TCA cycle elevated taurine are identified as key pathways most crucial biomarker reprogrammed...
Sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) functions as a unique redox-driven sodium pump, generating membrane potential, which is related to aminoglycoside antibiotic resistance. However, whether it modulates other metabolisms confer resistance unknown. The present study showed that loss of nqrA or nqrF led differential metabolomes with elevated antibiotics. Decreased alanine, aspartate, and glutamate metabolism depressed abundance alanine were characterized the most...
Abstract Background Airway inflammation is the core pathological process of asthma, with key inflammatory regulators incompletely defined. Recently, fibroblast growth factor 2 (FGF2) has been reported to be an regulator; however, its role in asthma remains elusive. This study aimed investigate immunomodulatory FGF2 asthma. Methods First, expression was characterised clinical samples and house dust mite (HDM)-induced mouse chronic model. Second, recombinant (rm-FGF2) protein intranasally...
Temperature is one of the major climate factors that affects outbreak infectious disease. Lines evidences have shown virulence can be controlled by thermo-sensors in bacterial pathogens. However, how temperature influence host's responses to pathogen still largely unexplored, and study on which might pave way develop strategies manage pathogenic infection. In present study, we show finfish Carassius carassius, crucian carp tolerant a wide range temperature, less susceptible infection when...
Summary Vibrio alginolyticus threatens both humans and marine animals, but hosts respond to V. infection is not fully understood. Here, functional metabolomics was adopted investigate the metabolic differences between dying surviving zebrafish upon infection. Tryptophan identified as most crucial metabolite, whose abundance decreased in group increased survival compared control without Concurrently, displayed excessive immune response produced higher level of reactive oxygen species (ROS)....
Infections with Pseudomonas aeruginosa have become a real concern among hospital-acquired infections, especially in cystic fibrosis patients and immunocompromised individuals. Control of the pathogen is challenging due to antibiotic resistance.
Multidrug-resistant Pseudomonas aeruginosa has become one of global threat pathogens for human health due to insensitivity antibiotics. Recently developed reprogramming metabolomics can identify biomarkers, and then, the biomarkers were used revert elevate antibiotic-mediated killing. Here, methodology was study cefoperazone/sulbactam (SCF)-resistant P. (PA-RSCF) identified reduced glycolysis pyruvate cycle, a recent clarified cycle providing respiratory energy in bacteria, as most key...
To investigate the prevalence and trends of sensitisation to common aeroallergens among outpatients with allergic rhinitis (AR) in Guangzhou, China, over past decade.
Th17 and regulatory T cell (Treg) play crucial roles in the pathogenesis of asthma. However, association between Th17/Treg homeostasis asthma exacerbation remains unclear.To investigate role bias exacerbation, 49 patients were enrolled current study, whom 31 had acute (exacerbation group) 18 did not (non-exacerbation group). Meanwhile, 17 healthy subjects recruited as normal controls (control By measuring interleukin (IL)-4, IL-13, interferon (IFN)-γ, IL-10, IL-17A levels plasma using...
Summary Understanding the interplay between bacterial fitness, antibiotic resistance, host immunity and metabolism could guide treatment improve against antibiotic‐resistant pathogens. The acquisition of levofloxacin (Lev) resistance affects fitness Vibrio alginolyticus in vitro vivo . Lev‐resistant (Lev‐R) V. exhibits slow growth, reduced pathogenicity greater to killing by host, Danio rerio (zebrafish), than Lev‐sensitive (Lev‐S) , suggesting that Lev‐R triggers a weaker innate immune...
The present study explored the cooperative effect of both alanine (Ala) and gentamicin (Gent) on metabolic mechanisms by which exogenous Ala potentiates Gent to kill antibiotic-resistant Vibrio alginolyticus. To test this, GC-MS-based metabolomics was used characterize Ala-, Gent- both-induced profiles, identifying nitric oxide (NO) production pathway as most key clue understand mechanisms. Gent, led low, lower lowest activity total synthase (tNOS) level NO, respectively. NOS promoter...
Premature infants have a high risk of bronchopulmonary dysplasia (BPD), which is characterized by abnormal development alveoli and pulmonary vessels. Exosomes exosomal miRNAs (EXO-miRNAs) from bronchoalveolar lavage fluid are involved in the BPD might serve as predictive biomarkers for BPD. However, roles exosomes EXO-miRNAs umbilical cord blood regulating angiogenesis yet to be elucidated. In this study, we showed that blood-derived impaired vitro. Next-generation sequencing preterm without...
Overactive immune response is a critical factor triggering host death upon bacterial infection. However, the mechanism behind regulation of excessive responses still largely unknown, and corresponding control preventive measures are to be explored. In this study, we find that Nile tilapia, Oreochromis niloticus, died from Edwardsiella tarda infection had higher levels than those survived. Such strongly associated with metabolism was altered at 6 h postinfection. By gas chromatography-mass...
Antibiotic-resistant Pseudomonas aeruginosa has become a real concern in hospital-acquired infections, especially critically ill and immunocompromised patients. Understanding antibiotic resistance mechanisms developing novel control measures are highly appreciated.
Evasion of complement-mediated killing is a common phenotype for many different types pathogens, but the mechanism still poorly understood. Most clinic isolates Edwardsiella tarda, an important pathogen infecting both human and fish, are commonly found serum-resistant. To explore potential mechanisms, we applied gas chromatography-mass spectrometry (GC-MS)-based metabolomics approaches to profile metabolomes E. tarda EIB202 in presence or absence serum stress. We that tricarboxylic acid...
Abstract Background No comprehensive analysis is available on the viral etiology and clinical characterization among children with severe acute lower respiratory tract infection (SALRTI) in Southern China. Methods Cohort of 659 hospitalized (2 months to 14 years) SALRTI admitted Pediatric Intensive Care Unit (PICU) Guangzhou from May 2015 April 2018 was enrolled this study. Nasopharyngeal aspirate specimens or induced sputum were tested for eight categories targets. The distribution its...