A5034051735- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Genomics and Chromatin Dynamics
- Glioma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Body Contouring and Surgery
- RNA modifications and cancer
- Electrospun Nanofibers in Biomedical Applications
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Protease and Inhibitor Mechanisms
- Systemic Sclerosis and Related Diseases
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- HER2/EGFR in Cancer Research
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
Health Research Institute of the Balearic Islands
2020-2023
Atrium Health Wake Forest Baptist
2022
Wake Forest University
2021
Florida College
2019-2020
University of Florida
2019-2020
Institut Català d'Oncologia
2020
Fondazione Lombardia per l’Ambiente
2019
Center of Regenerative Medicine in Barcelona
2011-2016
There are seven linker histone variants in human somatic cells (H1.0 to H1.5 and H1X), their prevalence varies as a function of cell type differentiation stage, suggesting that the different may have distinct roles. We revisited this notion by using new methodologies study pluripotency differentiation, including vitro embryonic stem (ES) teratocarcinoma reprogramming keratinocytes induced pluripotent cells. Our results show (PCs) decreased levels H1.0 increased H1.1, H1.3, compared with...
Transcription-factor-induced reprogramming of somatic cells to pluripotency is a very inefficient process, probably due the existence important epigenetic barriers that are imposed during differentiation and contribute preserving cell identity. In an effort decipher molecular nature these barriers, we followed genome-wide approach, in which identified macrohistone variants (macroH2A) as highly expressed human but downregulated after pluripotency, well strongly induced differentiation....
Background: Recent technology developed by Tulip Medical Products allows clinicians to mechanically disaggregate fat tissue into small particles known as nanofat. The present study aimed evaluate the cell yield obtained from nanofat generation in comparison traditional methods involving enzymatic dissociation (stromal vascular fraction). Methods: Nanofat preparations were characterized content and viability, based on DNA quantification image cytometry, respectively. analysis was also used...
Histone modifying enzymes play critical roles in cell differentiation and development. In this study, we report that SMYD2 (SET MYND domain containing protein 2), a histone lysine methyltransferase, is induced during human embryonic stem (ES) it preferentially expressed somatic cells versus pluripotent cells. Knockdown of ES promotes the induction endodermal markers differentiation, while overexpression has opposite effects. <I>In vivo </I>experiments zebrafish revealed knockdown...
Abstract Background Triple-negative breast cancer (TNBC) is an aggressive subtype that exhibits a high incidence of distant metastases and lacks targeted therapeutic options. Here we explored how the epigenome contributes to matrix metalloprotease (MMP) dysregulation impacting tumor invasion, which first step metastatic process. Methods We combined RNA expression chromatin interaction data identify insulator elements potentially associated with MMP gene invasion. employed CRISPR/Cas9 disrupt...
The successful use of specialized cells in regenerative medicine requires an optimization the differentiation protocols that are currently used. Understanding molecular events take place during human pluripotent is essential for improvement these and generation high quality differentiated cells. In effort to understand mechanisms govern we identify methyltransferase SETD7 as highly induced embryonic stem differentially expressed between somatic Knock-down causes defects cell including delay...
Abstract Glioblastoma (GBM) is the most aggressive primary brain tumor, having a poor prognosis and median overall survival of less than two years. Over last decade, numerous findings regarding distinct molecular genetic profiles GBM have led to emergence several therapeutic approaches. Unfortunately, none them has proven be effective against progression recurrence. Epigenetic mechanisms underlying tumor biology, including histone modifications, DNA methylation, chromatin architecture,...
Abstract Background Glioblastoma (GBM) is the most aggressive and prevalent primary brain tumor, with a median survival of 15 months. Advancements in multi-omics profiling combined computational algorithms have unraveled existence three GBM molecular subtypes (Classical, Mesenchymal, Proneural) clinical relevance. However, due to costs high-throughput techniques, subtyping not currently employed settings. Methods Using Random Forest Nearest Shrunken Centroid algorithms, we constructed...
Glioma stem cells (GSCs) have self-renewal and tumor-initiating capacities involved in drug resistance immune evasion mechanisms glioblastoma (GBM).Core-GSCs (c-GSCs) were identified by selecting co-expressing high levels of embryonic cell (ESC) markers from a single-cell RNA-seq patient-derived GBM dataset (n = 28). Induced c-GSCs (ic-GSCs) generated reprogramming GBM-derived (GBM-DCs) using induced pluripotent (iPSC) technology. The characterization ic-GSCs GBM-DCs was conducted...
Regenerative cell strategies rely on stromal implants to attain an observable clinical outcome. However, the effective dose ensure a therapeutic response remains unknown. To achieve higher dose, authors hypothesized that reducing volume occupied by mature adipocytes in lipoaspirate will concentrate vascular fraction present original tissue.
Introduction The impact of fat grafting on the viscoelasticity irradiated tissues is poorly defined. We investigate effect subcutaneous postmastectomy tissue expansion in patients undergoing delayed breast reconstruction. quantify observed viscoelastic and trophic changes skin envelope. hypothesize that properties soft Methods Postmastectomy defects more than 2 years reconstructed with subpectoral expanders were prospectively studied. Control (no irradiation, no grafting, n = 7), grafted 8),...
Abstract Background Triple-negative breast cancer (TNBC) is an aggressive subtype that exhibits a high incidence of distant metastases and lacks targeted therapeutic options. Here we explored how the epigenome may contribute to matrix metalloprotease (MMP) dysregulation given their key role in invasion, which first step metastatic process. Methods We combined RNA expression chromatin interaction data identify insulator elements potentially associated with invasion. stably disrupted...
PURPOSE: Fat Pulmonary Embolism Syndrome (F-PES) complicates high-volume fat grafting. Adipocyte disruption generates triglyceride (TGC) oil dispersed in lipoaspirate (lipocrit). TGC plasma concentration ([TGC]p) was compared with lipocrit during liposuction-lipografting procedures. 1 buttock grafting case suffered a F-PES that required ICU support. METHODS: n=27 (19 breast, 8 buttocks) recorded and measured peripheral blood samples preoperatively, intra 24h postoperatively. patient...
Abstract Glioma stem cells (GSCs) are a subset of with self-renewal and tumor-initiating capacities that thought to participate in drug resistance immune evasion mechanisms glioblastoma (GBM). Given GBM heterogeneity, we hypothesized GSCs might also display cellular hierarchies associated different degrees stemness. We evaluated single-cell RNA-seq dataset ( n = 28) identified cell population co-expressing high levels embryonic pluripotency markers, named core glioma (c-GSCs). This...