A5024535210- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Redox biology and oxidative stress
- Antimicrobial Resistance in Staphylococcus
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Ocean Acidification Effects and Responses
- Plant-Microbe Interactions and Immunity
- Heat shock proteins research
- Echinoderm biology and ecology
- Gene Regulatory Network Analysis
- RNA regulation and disease
- Biochemical effects in animals
- Meat and Animal Product Quality
- Protein Kinase Regulation and GTPase Signaling
- Cell death mechanisms and regulation
- Folate and B Vitamins Research
- Plant Pathogenic Bacteria Studies
- DNA and Nucleic Acid Chemistry
University of Wollongong
2020-2022
Illawarra Health and Medical Research Institute
2020-2022
University of Otago
2016-2021
Allosteric regulation of the pseudokinase TRIB1 reveals therapeutic potential kinase inhibitors.
Abstract Chemoreceptors enable bacteria to detect chemical signals in the environment and navigate towards niches that are favourable for survival. The sensor domains of chemoreceptors function as input modules chemotaxis systems, provide sensory specificity by binding specific ligands. Cache-like most common extracellular module prokaryotes, however only a handful have been functionally or structurally characterised. Here, we characterised chemoreceptor domain (PscD-SD) from plant pathogen...
The first cells probably possessed rudimentary metabolic networks, built using a handful of multifunctional enzymes. promiscuous activities modern enzymes are often assumed to be relics this primordial era; however, by definition these no longer physiological. There many fewer examples single active site catalyze multiple physiologically-relevant reactions. Previously, we characterized the alanine racemase (ALR) activity Escherichia coli cystathionine β-lyase (CBL). Now have discovered that...
Apoptosis signal-regulating kinases (ASK1, ASK2, and ASK3) are activators of the p38 c-Jun N-terminal kinase (JNK) mitogen-activated protein (MAPK) pathways. ASK1-3 form oligomeric complexes known as ASK signalosomes that initiate signaling cascades in response to diverse stress stimuli. Here, we demonstrated oligomerization proteins is driven by previously uncharacterized sterile-alpha motif (SAM) domains reside at carboxy-terminus each protein. SAM from exhibited distinct behaviors, with...
Bacterial methionine biosynthesis can take place by either the trans-sulfurylation route or direct sulfurylation. The enzymes responsible for have been characterized extensively because they occur in model organisms such as Escherichia coli. However, sulfurylation is actually predominant across phylogenetic tree. In this pathway, most bacteria use an O-acetylhomoserine aminocarboxypropyltransferase (MetY) to catalyze formation of homocysteine from and bisulfide. Despite widespread...
The Redβ protein of the bacteriophage λ red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present structure helical oligomeric intermediate Redβ, consisting N-terminal residues 1-177 bound to two complementary 27mer oligonucleotides, determined via cryogenic electron microscopy (cryo-EM) final resolution 3.3 Å. reveals continuous binding groove positions and stabilizes strands in planar orientation facilitate base...
Staphylococcus aureus is a prevalent bacterial pathogen in both community and hospital settings, its treatment made particularly difficult by resilience within biofilms. Within this niche, serine hydrolase enzymes play key role generating maintaining the biofilm matrix. Activity-based profiling has previously identified family of hydrolases, designated fluorophosphonate-binding hydrolases (Fph's), some which contribute to virulence S. vivo. These 10 Fph proteins have limited annotation few,...
Summary Novel bacterial ( HT ) and fungal FPII food‐grade protease preparations were evaluated for their ability to hydrolyse sheep cheese whey SCW the generation of bioactive peptides. Both hydrolysed proteins small peptides over 24‐h hydrolysis time, but time course profiles different as by SDS ‐ PAGE . The hydrolysate had considerably higher antioxidant angiotensin‐I converting enzyme ACE )‐inhibitor activity than hydrolysate. Neither was cytotoxic towards Vero cells. OFFGEL...
Nitrate- and nitrite-sensing (NIT) domains are found associated with a wide variety of bacterial receptors, including chemoreceptors. However, the structure chemoreceptor-associated NIT domain has not yet been characterized. Recently, chemoreceptor named PscF was identified from plant pathogen Pseudomonas syringae pv. actinidiae that is predicted to contain periplasmic domain. The sensor (PscF-SD; residues 42–332) cloned into an appropriate expression vector, recombinantly produced in...
One Sentence Summary Substrate binding to Tribbles-homolog 1 (TRIB1) pseudokinase induces allosteric changes that allow formation of a complex with the COP1 ubiquitin ligase. Abstract The Tribbles family pseudokinases recruit substrates ligase for ubiquitination. CCAAT-enhancer protein (C/EBP) transcription factors are crucial in adipocyte and myeloid development. Here we show TRIB1 can various C/EBP members, C/EBPβ attenuated by phosphorylation. To explain mechanism substrate recruitment,...
Sea urchins are commercially harvested for their edible gonads, also known as roe or uni. Most species, particularly those from the Northern hemisphere reported to exhibit limited variation in gonad colour, which is proposed be due carotenoid pigments. The species New Zealand, Evechinus chloroticus, and Australia, Heliocidaris erythrogramma, however, considerable colour variation, ranging a culinary desirable light yellow/orange an undesirable brown. Gonads E. chloroticus H. erythrogramma...
Abstract Apoptosis signal-regulating kinases (ASK1–3) are activators of the P38 and JNK MAP kinase pathways. ASK1–3 form oligomeric complexes known as ASK signalosomes that initiate signalling cascades in response to diverse stress stimuli. Here we demonstrate oligomerization proteins is driven by previously uncharacterised sterile-alpha motif (SAM) domains reside at C-terminus each protein. SAM from have distinct behaviours: ASK1 forms unstable oligomers, ASK2 predominantly monomeric, ASK3...
Summary Staphylococcus aureus is a major cause of infection in the community and hospitals. Serine hydrolases play key roles bacterial homeostasis, particular biofilms. Activity-based profiling has previously identified family serine hydrolases, designated fluorophosphonate-binding (Fphs), which contribute to virulence S. biofilm niche. Here we report structures putative tributyrin esterase FphF, alone covalently bound by substrate analog, small molecule inhibitors that occupy hydrophobic...
Abstract The bacteriophage λ red recombination system catalyzes the single-strand annealing homologous DNA reaction, in which Redβ annealase protein plays a critical role. Using cryogenic electron microscopy, we were able to determine structure of C-terminally truncated with residues 1-177 bound two complementary 27mer oligonucleotides forming an intermediate, final resolution 3.3 Å. This validates and rationalizes decades experimental observations on biochemistry Redβ. Definition...