A5069749806- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Extracellular vesicles in disease
- Parasite Biology and Host Interactions
- Silk-based biomaterials and applications
- Insect and Arachnid Ecology and Behavior
- Silkworms and Sericulture Research
- Photoreceptor and optogenetics research
- Plant Stress Responses and Tolerance
- Wnt/β-catenin signaling in development and cancer
- MXene and MAX Phase Materials
- Genomics and Phylogenetic Studies
- Insect-Plant Interactions and Control
- Parasites and Host Interactions
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Microbial Community Ecology and Physiology
- Mass Spectrometry Techniques and Applications
- Fibroblast Growth Factor Research
- ATP Synthase and ATPases Research
- Fungal Plant Pathogen Control
- Plant nutrient uptake and metabolism
- Genetics and Neurodevelopmental Disorders
University of South Bohemia in České Budějovice
2015-2024
Czech Academy of Sciences
2018-2023
Czech Academy of Sciences, Institute of Microbiology
2016-2023
Institute of Parasitology
2006-2013
Czech Academy of Sciences, Biology Centre
2013
Sewanee: The University of the South
2011
Macromolecular membrane assemblies of chlorophyll-protein complexes efficiently harvest and trap light energy for photosynthesis. To investigate the delivery chlorophylls to newly synthesized photosystem apoproteins, a terminal enzyme chlorophyll biosynthesis, synthase (ChlG), was tagged in cyanobacterium Synechocystis PCC 6803 (Synechocystis) used as bait pull-down experiments. We retrieved an enzymatically active complex comprising ChlG high-light-inducible protein HliD, which associates...
Abstract Efficient assembly and repair of the oxygen-evolving photosystem II (PSII) complex is vital for maintaining photosynthetic activity in plants, algae, cyanobacteria. How chlorophyll delivered to PSII during how vulnerable complexes are protected from photodamage unknown. Here, we identify a β-carotene binding protein cyanobacterium Synechocystis PCC 6803 important formation D1/D2 reaction center complex. It composed putative short-chain dehydrogenase/reductase Ycf39, encoded by...
Dishevelled (Dvl) is a key component in the Wnt/β-catenin signaling pathway. Dvl can multimerize to form dynamic protein aggregates, which are required for activation of downstream signaling. Upon pathway by Wnts, becomes phosphorylated yield and shifted (PS) Dvl. Both Wnt-induced PS-Dvl formation dependent on casein kinase 1 (CK1) δ/ε activity. However, overexpression CK1 was shown dissolve endogenous forms irrespective whether or not activating Wnt triggers Using combination...
Lysine hydroxylation of type I collagen telopeptides varies from tissue to tissue, and these distinct patterns modulate cross-linking generate a unique extracellular matrix. Abnormalities in contribute pathologies that include osteogenesis imperfecta (OI), fibrosis, cancer. Telopeptide procollagen modifications are carried out by lysyl hydroxylase 2 (LH2); however, little is known regarding how this enzyme regulates patterns. We identified an ER complex resident chaperones includes HSP47,...
The canonical photosynthetic plastid genomes consist of a single circular-mapping chromosome that encodes highly conserved protein core, involved in photosynthesis and ATP generation. Here, we demonstrate the genome relative apicomplexans, Chromera velia, departs from this view several unique ways. Core proteins PsaA AtpB have been broken into two fragments, which show are independently transcribed, oligoU-tailed, translated, assembled functional photosystem I synthase complexes. Genome-wide...
Understanding the mechanisms that drive differentiation of dopaminergic (DA) neurons is crucial for successful development novel therapies Parkinson's disease, in which DA progressively degenerate. However, underlying differentiation-promoting effects Wnt5a on precursors are poorly understood. Here, we present molecular and functional characterization a signaling pathway downstream Wnt5a, Wnt/Dvl/Rac1 pathway. First, characterize interaction between Rac1 Dvl identify N-terminal part Dvl3 as...
One strategy for enhancing photosynthesis in crop plants is to improve their ability repair photosystem II (PSII) response irreversible damage by light. Despite the pivotal role of thylakoid-embedded FtsH protease complexes selective degradation PSII subunits during repair, little known about factors involved regulating expression. Here we show using cyanobacterium Synechocystis sp. PCC 6803 that Psb29 subunit, originally identified as a minor component His-tagged preparations, physically...
Significance A properly functioning primary cilium is prerequisite for both normal development and aging of all ciliated organisms, including humans. In vertebrates, the signaling Hedgehog family morphogens depends entirely on cilium. Recently, we reported that fibroblast growth factors (FGF) interacts with Hedgehog, this a consequence FGF regulating length speed processes happen therein. report, provide molecular mechanism such interaction, identifying intestinal cell kinase as mediator...
Although the PSII complex is highly conserved in cyanobacteria and chloroplasts, PsbU PsbV subunits stabilizing oxygen-evolving Mn4CaO5 cluster are absent chloroplasts have been replaced by PsbP PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, unknown function. Here we show that CyanoP plays role early stages biogenesis Synechocystis sp. PCC 6803. present reaction center assembly (RCII) lacking both CP47 CP43 modules binds to smaller D2 module. A...
Photosystem II (PSII) is a large enzyme complex embedded in the thylakoid membrane of oxygenic phototrophs. The biogenesis PSII requires assembly more than 30 subunits, with assistance number auxiliary proteins. In plants and cyanobacteria, photosynthesis-affected mutant 68 (Pam68) important for assembly. However, its mechanisms action remain unknown. Using Synechocystis PCC 6803 strain expressing Flag-tagged Pam68, we purified protein containing ribosomes, SecY translocase,...
The silks produced by caterpillars consist of fibroin proteins that form two core filaments, and sericin seal filaments into a fiber conglutinate fibers in the cocoon. Sericin genes are well-known Bombyx mori (Bombycidae) but have received little attention other insects. This paper shows Antheraea yamamai (Saturniidae) contains five very different from three B. mori. In spite differences, all known sericins characterized short exons 1 2 (out 3-12 exons), expression middle silk gland section,...
Lepidopteran silk is a complex assembly of proteins produced by pair highly specialized labial glands called glands. Silk composition has been examined only in handful species. Here we report on the analysis gland-specific transcriptomes from three developmental stages greater wax moth, Galleria mellonella, combined with proteomics, Edman microsequencing and northern blot analysis. In addition to genes known earlier, identified twenty seven candidate cDNAs predicted encode secretory...
In oxygenic photosynthesis the initial photochemical processes are carried out by photosystem I (PSI) and II (PSII). Although subunit composition varies between cyanobacterial plastid photosystems, core structures of PSI PSII conserved throughout photosynthetic eukaryotes. So far, complexes have been characterised in only a small number organisms. We performed silico biochemical studies to explore organization evolution apparatus chromerids Chromera velia Vitrella brassicaformis, autotrophic...
Summary Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for stability photosystem I (PSI) in cyanobacteria; however, how stabilized PSI remains unexplained. To clarify role BtpA, we constructed and btpA‐ null mutant (Δ btpA ) cyanobacterium Synechocystis sp. PCC 6803. contained only c . 1% chlorophyll nearly no thylakoid...
Abstract Background Helminth extracellular vesicles (EVs) are known to have a three-way communication function among parasitic helminths, their host and the host-associated microbiota. They considered biological containers that may carry virulence factors, being therefore appealing as therapeutic prophylactic target candidates. This study aims describe characterise EVs secreted by Sparicotyle chrysophrii (Polyopisthocotyla: Microcotylidae), blood-feeding gill parasite of gilthead seabream (...