A5030161874- Pharmacogenetics and Drug Metabolism
- 3D Printing in Biomedical Research
- Dialysis and Renal Disease Management
- Diet and metabolism studies
- Birth, Development, and Health
- Liver physiology and pathology
- Receptor Mechanisms and Signaling
- Acute Kidney Injury Research
- HIV/AIDS drug development and treatment
- Eicosanoids and Hypertension Pharmacology
- Biomedical Ethics and Regulation
- Computational Drug Discovery Methods
- Pluripotent Stem Cells Research
- Renal and related cancers
- Neurotransmitter Receptor Influence on Behavior
- Pharmacological Effects and Toxicity Studies
- Microfluidic and Capillary Electrophoresis Applications
- Ion Transport and Channel Regulation
- Drug Transport and Resistance Mechanisms
- Barrier Structure and Function Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Chronic Kidney Disease and Diabetes
Utrecht University
2023-2025
University of Manchester
2019-2022
Manchester Academic Health Science Centre
2021-2022
Radboud University Nijmegen
2016
Radboud University Medical Center
2016
Radboud Institute for Molecular Life Sciences
2016
Drug-induced nephrotoxicity still hampers drug development, because current translation from in vitro or animal studies to human lacks high predictivity. Often, renal adverse effects are recognized only during clinical stages of development. The study aimed establish a robust and more complete cell model suitable for screening drug-related interactions nephrotoxicity. In addition endogenously expressed organic cation transporters efflux transporters, conditionally immortalized proximal...
Indoxyl sulfate (IxS), a highly albumin-bound uremic solute, accumulates in chronic kidney disease (CKD) due to reduced renal clearance. This study was designed specifically investigate the role of human serum albumin (HSA) IxS secretion via organic anion transporter 1 (OAT1) microfluidic system and subsequently apply quantitative translation vitro data predict extent change clearance CKD stage IV relative healthy. Conditionally immortalized proximal tubule epithelial cells overexpressing...
The organic anion transporter-1 (OAT1), located at the basal side of kidney proximal tubule cells, is actively involved in metabolic waste excretion. During chronic disease (CKD), progressive decline renal function, results accumulation endogenous metabolites bloodstream, exacerbating comorbidities. CKD also leads to gut dysbiosis, resulting an increased production uremic and a reduced nephroprotective short chain fatty acid (SCFAs), mainly acetate, propionate butyrate, thereby contributing...
Abstract Liver-on-a-chip models predictive for both metabolism, and blood canalicular transport of drug candidates in humans are lacking. Here, we established a bioengineered 3Rs-complied (animal component-free) hepatocyte-like millifluidic system based on 3D hollow fiber membranes (HFMs), recombinant human laminin 332 coating adult stem cell-derived organoids. Organoid fragments formed polarized tight monolayers HFMs with improved maturation, as compared to standard organoid cultures...
Abstract Liver-on-a-chip models predictive for both metabolism as well canalicular and blood transport of drug candidates in humans are lacking. Here, we established an advanced, bioengineered animal component-free hepatocyte-like millifluidic system based on 3D hollow fiber membranes (HFMs), recombinant human laminin 332 coating adult stem cell-derived organoids. Organoid fragments formed polarized tight monolayers HFMs with improved maturation, compared to standard organoid cultures...
Active tubular secretion of drugs from the blood to urine is mediated by multiple drug transporters expressed on both basolateral and apical membrane proximal tubule. Human tubule microphysiological system (MPS) has been proposed as a more physiologically relevant in vitro assess nephrotoxicity reabsorption.[1,2] However, drug-transporter studies MPSs are currently sparse quantitative translation MPS data vivo not yet established. This study aimed feasibility NORTIS quantitatively...