A5079633498- Ion Channels and Receptors
- Ion channel regulation and function
- Plant Pathogenic Bacteria Studies
- Neurobiology and Insect Physiology Research
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Magnesium in Health and Disease
- Legume Nitrogen Fixing Symbiosis
- Respiratory and Cough-Related Research
- Microbial Metabolic Engineering and Bioproduction
- Plant Stress Responses and Tolerance
- Cardiac electrophysiology and arrhythmias
- Biochemical Analysis and Sensing Techniques
- Plant responses to water stress
- Enzyme Catalysis and Immobilization
- Immune Cell Function and Interaction
- Analytical Chemistry and Sensors
- Plant Genetic and Mutation Studies
- Helminth infection and control
- Parasites and Host Interactions
- Piperaceae Chemical and Biological Studies
- Circadian rhythm and melatonin
- Plant biochemistry and biosynthesis
La Jolla Institute for Immunology
2011-2020
Council of Scientific and Industrial Research
2008-2011
Centre for Cellular and Molecular Biology
2007-2011
Xanthomonas oryzae pv. causes bacterial blight of rice (Oryza sativa L.), a major disease that constrains production this staple crop in many parts the world. We report here on complete genome sequence strain PXO99A and its comparison to two previously sequenced strains, KACC10331 MAFF311018, which are highly similar one another.The is single circular chromosome 5,240,075 bp, considerably longer than genomes other strains (4,941,439 bp 4,940,217 respectively), it contains 5083 protein-coding...
ABSTRACT Xanthomonas is a large genus of bacteria that collectively cause disease on more than 300 plant species. The broad host range the contrasts with stringent and tissue specificity for individual species pathovars. Whole-genome sequences campestris pv. raphani strain 756C X. oryzae oryzicola BLS256, pathogens infect mesophyll leading models biology, Arabidopsis thaliana rice, respectively, were determined provided insight into genetic determinants specificity. Comparisons made genomes...
STIM1 and STIM2 are endoplasmic reticulum (ER) membrane proteins that sense decreases in ER-luminal free Ca2+ and, through a conformational change the STIM cytoplasmic domain, control gating of plasma channel ORAI1. To determine how conveys signal from ER lumen to cytoplasm, we studied Ca2+-dependent engineered isolated membranes parallel, physiological activation these cells. We find conserved "sentinel" features CC1 region help prevent while is bound domains. Reduced drives concerted...
Significance Close appositions between the endoplasmic reticulum (ER) and plasma membrane in mammalian cells have essential roles cellular lipid metabolism cytoplasmic calcium signaling. Although recent investigations yielded considerable insight into structural basis for transfer at ER–plasma junctions, little is known about proteins that organize junctions Our data show ER protein transmembrane 110 (TMEM110) supports maintenance of competent signaling acts concert with other dynamic...
Abstract Stromal interaction molecule 1 (STIM1) monitors ER-luminal Ca 2+ levels to maintain cellular balance and support signalling. The prevailing view has been that STIM1 senses reduced ER through dissociation of bound from a single EF-hand site, which triggers dramatic loss secondary structure dimerization the luminal domain. Here we find domain 5–6 -binding sites, binding at these sites is energetically coupled controls switch second structured conformation rather than protein...
Xanthomonas oryzae pv (Xoo) causes bacterial blight, a serious disease of rice (Oryza sativa). LipA is secretory virulence factor Xoo, implicated in degradation cell walls and the concomitant elicitation innate immune responses, such as callose deposition programmed death. Here, we present high-resolution structural characterization that reveals an all-helical ligand binding module distinct functional attachment to canonical hydrolase catalytic domain. We demonstrate enzyme binds glycoside...
The transcription factor nuclear of activated T cells (NFAT) has a key role in both cell activation and tolerance emerged as an important target immune modulation. NFAT directs the effector arm response presence activator protein-1 (AP-1), anergy/exhaustion absence AP-1. Envisioning strategy for selective modulation response, we designed FRET-based high-throughput screen to identify compounds that disrupt NFAT:AP-1:DNA complex. We screened ∼202,000 small organic identified 337 candidate...
Significance L-type Ca 2+ channel (Ca v 1.2) blockers (LCCBs) represent a large family of drugs widely used in the clinic for over 70 y to treat hypertension, angina, and cardiac arrhythmias. Using genetically modified cells, animal models, human studies, we demonstrate that all three major classes LCCBs activate STIM proteins by acting on 10-amino acid N-terminal region located endoplasmic reticulum lumen. The activation triggers store-operated entry promotes vascular remodeling. These...
Ca2+ signaling is important for many cellular and physiological processes, including cardiac function. Although sarcoplasmic reticulum (SR) proteins involved in have been shown to be phosphorylated, the biochemical roles of protein phosphorylation within lumen SR remain essentially uncharacterized. Our laboratory recently identified an atypical kinase, Fam20C, which uniquely localized secretory pathway lumen. Here, we show that Fam20C phosphorylates several signaling, calsequestrin2 Stim1,...
Xanthomonas oryzae pv. is the causal agent of bacterial leaf blight, a serious disease rice. Several enzymes that are secreted through type II secretion system this bacterium play an important role in plant–microbe interaction, being for virulence and also able to induce potent host defence responses. One these secretory lipase/esterase, LipA, which shows very weak homology other lipases gives positive tributyrin plate assay. In study, LipA was purified from culture supernatant...