A5015882616- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Plant pathogens and resistance mechanisms
- Plant Disease Resistance and Genetics
- RNA Interference and Gene Delivery
- Plant Pathogens and Resistance
- Insect symbiosis and bacterial influences
- Plant tissue culture and regeneration
- Virus-based gene therapy research
- Research in Cotton Cultivation
- Banana Cultivation and Research
- RNA and protein synthesis mechanisms
- Innovation and Socioeconomic Development
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Mycotoxins in Agriculture and Food
- Chromosomal and Genetic Variations
- Animal Genetics and Reproduction
- Plant nutrient uptake and metabolism
Cornell University
2016-2025
Plant (United States)
2016-2025
Iowa State University
2008-2018
Doane University
2017
Western University
2014
University of Electronic Science and Technology of China
2011-2012
University of Minnesota
2012
Cellectis (United States)
2012
Fred Hutch Cancer Center
2012
Yale University
2012
TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors plant pathogenic Xanthomonas spp. to the FokI nuclease, bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays polymorphic amino acid repeats TAL effectors. We present a method reagents efficiently assembling TALEN constructs with custom repeat arrays. also describe design guidelines based on naturally occurring their binding sites. Using software...
TAL Order Xanthomonas bacteria attack their plant hosts by delivering own transcription-activator–like (TAL) proteins into the cell nucleus and alter plant's gene regulation (see Perspective Voytas Joung ). Moscou Bogdanove (p. 1501 , published online 29 October: see cover) Boch et al. 1509 October) have now discovered how similar but not identical repeats in encode specificity needed for to find targets. Each repeat is specific one DNA base pair, a encoded hypervariable amino acid...
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific created by fusing transcription activator-like effectors (TALEs) to the catalytic domain FokI endonuclease. Both native and custom TALE-nuclease fusions direct double-strand breaks specific, sites.
SUMMARY Xanthomonas oryzae pv. and oryzicola cause bacterial blight leaf streak of rice (Oryza sativa), which constrain production this staple crop in much Asia parts Africa. Tremendous progress has been made characterizing the diseases breeding for resistance. X. causes by invading vascular tissue, while colonizing parenchyma. In there are 29 major genes resistance to blight, but so far only a few quantitative loci streak. Over 30 races have reported. Both pathogens exhibit genetic...
Transcription activator-like (TAL) effectors are repeat-containing proteins used by plant pathogenic bacteria to manipulate host gene expression. Repeats polymorphic and individually specify single nucleotides in the DNA target, with some degeneracy. A TAL effector-nucleotide binding code that links repeat type specified nucleotide enables prediction of genomic sites for customization use targeting, particular as custom transcription factors engineered regulation site-specific nucleases...
Wrapped DNA TAL effectors are proteins that bacterial pathogens inject into plant cells bind to host activate expression of genes. The DNA-binding domain is composed tandem repeats within which a repeat-variable diresidue sequence confers nucleotide specificity. Deng et al. (p. 720 , published online 5 January) report the structure effector dHax3, containing 11.5 repeats, in DNA-free and DNA-bound states, Mak 716 PthXo1 effector, 22 bound its target. Together, structures reveal...
Abstract The ability to precisely engineer plant genomes offers much potential for advancing basic and applied biology. Here, we describe methods the targeted modification of using transcription activator-like effector nucleases (TALENs). Methods were optimized tobacco (Nicotiana tabacum) protoplasts TALENs targeting acetolactate synthase (ALS) gene. Optimal TALEN scaffolds identified a protoplast-based single-strand annealing assay in which cleavage creates functional yellow fluorescent...
Xanthomonas oryzae pv. causes bacterial blight of rice (Oryza sativa L.), a major disease that constrains production this staple crop in many parts the world. We report here on complete genome sequence strain PXO99A and its comparison to two previously sequenced strains, KACC10331 MAFF311018, which are highly similar one another.The is single circular chromosome 5,240,075 bp, considerably longer than genomes other strains (4,941,439 bp 4,940,217 respectively), it contains 5083 protein-coding...
Transcription activator-like (TAL) effectors from Xanthomonas citri subsp. malvacearum (Xcm) are essential for bacterial blight of cotton (BBC). Here, by combining transcriptome profiling with TAL effector-binding element (EBE) prediction, we show that GhSWEET10, encoding a functional sucrose transporter, is induced Avrb6, effector determining Xcm pathogenicity. Activation GhSWEET10 designer (dTALEs) restores virulence avrb6 deletion strains, whereas silencing compromises susceptibility to...
ABSTRACT Xanthomonas is a large genus of bacteria that collectively cause disease on more than 300 plant species. The broad host range the contrasts with stringent and tissue specificity for individual species pathovars. Whole-genome sequences campestris pv. raphani strain 756C X. oryzae oryzicola BLS256, pathogens infect mesophyll leading models biology, Arabidopsis thaliana rice, respectively, were determined provided insight into genetic determinants specificity. Comparisons made genomes...
Obligate biotrophic pathogens of plants must circumvent or counteract defenses to guarantee accommodation inside the host. To do so, they secrete a variety effectors that regulate host immunity and facilitate establishment pathogen feeding structures called haustoria. The barley powdery mildew fungus Blumeria graminis f. sp. hordei produces large number proteins predicted be secreted from Fifty these effector candidates (BEC) were screened by host-induced gene silencing (HIGS), eight...
Bacterial leaf streak of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an increasingly important yield constraint in this staple crop. A mesophyll colonizer, Xoc differs from X. (Xoo), which invades xylem to cause bacterial blight rice. Both produce multiple distinct TAL effectors, type III-delivered proteins that transactivate effector-specific host genes. effector finds its target(s) via a partially degenerate code whereby the modular amino acid sequence identifies nucleotide...
The giant sequoia (Sequoiadendron giganteum) of California are massive, long-lived trees that grow along the U.S. Sierra Nevada mountains. Genomic data limited in and producing a reference genome sequence has been an important goal to allow marker development for restoration management. Using deep-coverage Illumina Oxford Nanopore sequencing, combined with Dovetail chromosome conformation capture libraries, was assembled into eleven chromosome-scale scaffolds containing 8.125 Gbp sequence....
Long-read sequencing facilitates assembly of complex genomic regions. In plants, loci containing nucleotide-binding, leucine-rich repeat (NLR) disease resistance genes are an important example such NLR constitute one the largest gene families in plants and often clustered, evolving via duplication, contraction, transposition. We recently mapped Xo1 locus for to bacterial blight leaf streak, found American heirloom rice variety Carolina Gold Select, a region that Nipponbare reference genome...
• Xanthomonas transcription activator-like (TAL) effectors promote disease in plants by binding to and activating host susceptibility genes. Plants counter with TAL effector-activated executor resistance genes, which cause cell death block progression. We asked whether the functional specificity of an gene could be broadened adding different effector elements (EBEs) it. added six EBEs rice Xa27 gene, confers strains bacterial blight pathogen oryzae pv. (Xoo) that deliver AvrXa27. The...
The DNA binding domain of Transcription Activator-Like (TAL) effectors can easily be engineered to have new sequence specificities. Consequently, TAL effector proteins become important reagents for manipulating genomes in vivo. by is mediated arrays 34 amino acid repeats. In each repeat, one two acids (repeat variable di-residues, RVDs) contacts a base the target. RVDs with specificity C, T and A been described; however, among that target G, RVD NN also binds A, NK rare naturally occurring...