A5065363999- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Teleoperation and Haptic Systems
- Integrated Circuits and Semiconductor Failure Analysis
- Prion Diseases and Protein Misfolding
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Neuroscience and Neuropharmacology Research
- PARP inhibition in cancer therapy
- Barrier Structure and Function Studies
- Mast cells and histamine
- Erythropoietin and Anemia Treatment
- Lanthanide and Transition Metal Complexes
- Tactile and Sensory Interactions
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- Metal-Organic Frameworks: Synthesis and Applications
- Magnetism in coordination complexes
- Neonatal and fetal brain pathology
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Alzheimer's disease research and treatments
- Electron and X-Ray Spectroscopy Techniques
- Folate and B Vitamins Research
Cleveland Clinic
2024-2025
Case Western Reserve University
2022-2024
Kent State University
2018-2023
Cleveland Clinic Lerner College of Medicine
2022
Methionine metabolism is dysregulated in multiple sclerosis (MS). The methyl donor betaine depleted the MS brain where it linked to changes levels of histone H3 trimethylated on lysine 4 (H3K4me3) and mitochondrial impairment. We investigated effects replacing this cuprizone mouse model MS. Supplementation with restored epigenetic control alleviated neurological disability mice. Betaine increased methylation potential (SAM/SAH ratio), H3K4me3, enhanced neuronal respiration, prevented axonal...
The cuprizone induced animal model of demyelination is characterized by in many regions the brain with high levels corpus callosum as well changes neuronal function 4-6 weeks exposure. used a tool to study and subsequent degeneration therapeutic interventions on these effects. Historically, has been shown contain no alterations blood-brain barrier integrity, key feature diseases that affect central nervous system. Cuprizone generally administered for obtain maximal degeneration. However,...
To investigate the role of Toll-like Receptor 7 (TLR7) in development neuropsychiatric lupus (NPSLE) B6.Nba2 murine model SLE. TLR7-deficient mice were evaluated for NPSLE symptoms through behavioral testing with comparison groups wild-type NPSLE-prone and B6 controls. Behavioral results context biomarker data, including flow cytometry immune cell activation, enzyme-linked immunosorbent assays (ELISA) to measure serum cytokine autoantibody levels, autoantibodies targeted against double...
Visual system pathology has been established as a prominent feature in demyelinating diseases such multiple sclerosis (MS). Here, we present protocol for assessing regional the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy. We describe steps dissection, post-fixation, myelin detection, slide scanning of basic protein, analysis demyelination. then detail procedures fluorescent antibody staining, transmission microscopy (TEM), myelinated...
Insult to the central nervous system (CNS) results in an early inflammatory response, which can be exploited as initial indicator of neurological dysfunction. Nanoparticle drug delivery systems provide a mechanism increase uptake drugs into specific cell types CNS such microglia, resident macrophage responsible for innate immune response. In this study, we developed two nanoparticle-based carriers potential theranostic microglial cells. Poly(lactic-co-glycolic) acid (PLGA)- and l-tyrosine...
Abstract The lack of acid stability in the stomach and temporal when moving through gastrointestinal (GI) tract has made development oral magnetic resonance imaging (MRI) contrast agents based on platform Gd 3+ –complexes problematic.On other hand, negative enhancement produced by T 2 ‐weighted metal oxide nanoparticles (NPs) often renders image readout difficult. Biocompatible NPs manganese Prussian blue analog K Mn 3 [Fe II (CN) 6 ] exhibit extremely high under acidic conditions gastric...
Abstract Glial cells, including astrocytes, microglia, and oligodendrocytes, are brain cells that support dynamically interact with neurons each other. These intercellular dynamics undergo changes during stress disease states. In response to most forms of stress, astrocytes will some variation activation, meaning upregulation in certain proteins expressed secreted either upregulations or downregulations various constitutive normal functions. While types activation many contingent on the...
Multiple sclerosis (MS) is initially characterized by myelin and axonal damage in central nervous system white matter lesions, but their causal role synapse loss remains undefined. Gray atrophy also present early MS, making it unclear if synaptic alterations are driven demyelinating lesions or primary gray damage. Furthermore, whether pathology occurs secondary to independent of demyelination drive changes not clear. Here, we address reducing selectively manipulating glutamatergic signaling...
Complex neurodevelopmental disorders involve motor as well cognitive dysfunction and these impairments are associated with both cerebral cerebellar maturity. A network of connections between two brain regions is proposed to underlie impairments. The gray matter has a protracted developmental timeline compared the cortex, however, making association relay pathways unclear for disabilities. We show that population amoeboid microglia infiltrate white through fourth ventricular zone during early...