A5084359780- Urinary Tract Infections Management
- Urinary Bladder and Prostate Research
- Virus-based gene therapy research
- Pelvic floor disorders treatments
- Pediatric Urology and Nephrology Studies
- Gut microbiota and health
- Cellular Mechanics and Interactions
- Bladder and Urothelial Cancer Treatments
- Health and Medical Research Impacts
- Science Education and Perceptions
- scientometrics and bibliometrics research
- Climate Change Communication and Perception
- Animal Virus Infections Studies
- Science, Research, and Medicine
- Genetics, Bioinformatics, and Biomedical Research
- Microtubule and mitosis dynamics
- Interdisciplinary Research and Collaboration
- RNA Research and Splicing
- Cell death mechanisms and regulation
- Academic Publishing and Open Access
- Trauma and Emergency Care Studies
- interferon and immune responses
- Conferences and Exhibitions Management
- Biotechnology and Related Fields
- RNA Interference and Gene Delivery
University College London
2016-2025
MRC Laboratory for Molecular Cell Biology
2008-2024
Roland Hill (United Kingdom)
2015-2023
The Royal Free Hospital
2015-2023
Transnational Press London
2010-2013
Company of Biologists
2013
UCL Australia
2010-2011
Leiden University
2004
BioMed Central (United Kingdom)
2004
University of Washington
1994-2000
ABSTRACT Chronic lower urinary tract symptoms (LUTS), such as urgency and incontinence, are common, especially among the elderly, but their etiology is often obscure. Recent studies of acute infections implicated invasion by Escherichia coli into cytoplasm urothelial cells, with persistence long-term bacterial reservoirs, role infection in chronic LUTS unknown. We conducted a large prospective study eligible patients controls over 3-year period, comparing routine urine cultures planktonic...
Cell migration entails protrusion of lamellipodia, densely packed networks actin filaments at the cell front. Filaments are generated by nucleation, likely mediated Arp2/3 complex and its activator Scar/WAVE [1Campellone K.G. Welch M.D. A nucleator arms race: cellular control assembly.Nat. Rev. Mol. Biol. 2010; 11: 237-251Crossref PubMed Scopus (681) Google Scholar]. It is unclear whether formins contribute to lamellipodial filament nucleation or serve as elongators nucleated [2Ridley A.J....
Cell migration is essential during development and in human disease progression including cancer. Most cell studies concentrate on known or predicted components of pathways.
SummaryAs they enter mitosis, animal cells undergo profound actin-dependent changes in shape to become round. Here we identify the Cdk1 substrate, Ect2, as a central regulator of mitotic rounding, thus uncovering link between cell-cycle machinery that drives entry and its accompanying actin remodeling. Ect2 is RhoGEF plays well-established role formation actomyosin contractile ring at exit, through local activation RhoA. We find first becomes active prophase, when it exported from nucleus...
Bacterial urinary tract infections (UTI) are a major growing concern worldwide. Uropathogenic Escherichia coli has been shown to invade the urothelium during acute UTI in mice and humans, forming intracellular reservoirs that can evade antibiotics immune response, allowing recurrence at later date. Other bacterial species, such as Staphylococcus saprophyticus, Klebsiella pneumonia Salmonella enterica have also be invasive UTI. However, role of infection chronic causing more subtle lower...
The chicken anemia virus-derived protein Apoptin induces apoptosis specifically in human tumor and transformed cells not normal, untransformed cells. cell killing activity correlates with a predominantly nuclear localization of cells, whereas normal it is detected mainly cytoplasmic structures. To explore the role for Apoptin-induced death we employed mutagenesis strategy. First, demonstrated that C terminus contains bipartite-type signal. Strikingly, further investigation showed two...
The Drosophila melanogaster MICAL protein is essential for the neuronal growth cone machinery that functions through plexin- and semaphorin-mediated axonal signaling. also involved in regulating myofilament organization synaptic structures, serves as an actin disassembly factor downstream of plexin-mediated repulsion. In mammalian cells there are three known isoforms, MICAL1, MICAL2 MICAL3, well MICAL-like proteins MICAL-L1 MICAL-L2, but little their function, information comes almost...
During animal cell division, an actin-based ring cleaves the into two. Problems with this process can cause chromosome missegregation and defects in cytoplasmic inheritance partitioning of organelles, which turn are associated human diseases. Although much is known about how segregation coupled to way organelles coordinate their during daughter cells less well understood. Here, using a high-content live-imaging small interfering RNA screen, we identify Myosin-XIX (Myo19) as novel regulator...
Abstract Murine models describe a defined host/pathogen interaction for urinary tract infection, but human cell studies are scant. Although recent urothelial organoid promising, none demonstrate long-term tolerance to urine, the natural substrate of tissue and uropathogens that live there. We developed novel from progenitor cells which demonstrates key structural hallmarks biomarkers urothelium. After three weeks transwell culture with 100% urine at apical interface, stratified into multiple...
The development of new modalities for high-efficiency intracellular drug delivery is a priority number disease areas. One such area urinary tract infection (UTI), which one the most common infectious diseases globally and imposes an immense economic healthcare burden. Common uropathogenic bacteria have been shown to invade urothelial wall during acute UTI, forming latent reservoirs that can evade antimicrobials immune response. This behaviour likely facilitates high recurrence rates after...
Introduction Murine models of urinary tract infection (UTI) have improved our understanding host-pathogen interactions. However, given differences between rodent and human bladders which may modulate host bacterial response, including certain biomarkers, urothelial thickness the concentration urine, development new human-based is important to complement mouse studies provide a more complete picture UTI in patients. Methods We originally developed three-dimensional (3D) model was urine...
Feline leukemia virus (FeLV) is thought to induce neoplastic diseases in infected cats by a variety of mechanisms, including the transduction host proto-oncogenes. While FeLV recombinants that encode cellular sequences have been isolated from tumors naturally animals, acquisition an unrelated gene has never documented experimental infection. We recombinant proviruses encoding feline Notch2 thymic lymphoma DNA two inoculated with molecularly cloned FeLV-61E. Four genomes were identified,...
Apoptin, a chicken anemia virus-encoded protein, is thought to be activated by general tumor-specific pathway, because it induces apoptosis in large number of human tumor or transformed cells but not their normal, healthy counterparts. Here, we show that Apoptin phosphorylated robustly both<i>in vitro</i> and <i>in vivo</i> negligibly normal cells, map the site threonine 108. A gain-of-function point mutation (T108E) conferred upon ability accumulate nucleus kill implying phosphorylation key...
What's known on the subject? and What does study add? Microscopic pyuria is widely used as a surrogate marker of infection, although there little data supporting its use in patients who present with non‐acute LUTS . The effects urinary storage, preservation, laboratory methods to enhance leucocyte detection, are also unclear. This large, prospective highlights poor performance dipstick urine analysis, direct microscopy, markers UTI A series analyses examine handling processing test...
Midstream urine (MSU) culture remains the gold standard diagnostic test for confirming urinary tract infection (UTI). We previously showed that patients with chronic lower symptoms (LUTS) below cutoff on MSU may still harbor bacterial and their antibiotic treatment was associated symptom resolution.
Urinary tract infection is among the most common infections worldwide, typically studied in animals and cell lines with limited uropathogenic strains. Here, we assessed diverse bacterial species a human urothelial microtissue model exhibiting full stratification, differentiation, innate epithelial responses, urine tolerance. Several uropathogens invaded intracellularly, but also commensal
Although a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as systematic method to define metazoan actin based on visual phenotype. Using comparative screens cultured Drosophila human cells, generated phenotypic profiles for annotated together with bearing predicted domains. These clusters the known "actinome" were identify putative new core regulators,...
A novel and facile approach to prepare hybrid nanoparticle embedded polymer nanofibers using pressurised gyration is presented. Silver nanoparticles nylon were used in this work. The solution's physical properties, rotating speed the working pressure had a significant influence on fibre diameter morphology. Fibres range of 60–500 nm spun 10 wt.%, 15 wt.% 20 solutions these bead-free fibres processed under 0.2 MPa 0.3 rotational 36,000 rpm. 1–4 Ag was added case 50–150 prepared same...