A5100781753- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Genetics and Neurodevelopmental Disorders
- ATP Synthase and ATPases Research
- Hearing, Cochlea, Tinnitus, Genetics
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Identification and Quantification in Food
- Genomics and Rare Diseases
- Animal Disease Management and Epidemiology
- Vestibular and auditory disorders
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Axon Guidance and Neuronal Signaling
- RNA modifications and cancer
- Epilepsy research and treatment
- Developmental Biology and Gene Regulation
- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Ear Surgery and Otitis Media
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
Peking University
2014-2024
Chinese Academy of Agricultural Sciences
2022-2024
Ministry of Agriculture and Rural Affairs
2020-2024
Peking University First Hospital
2014-2024
Shanghai Veterinary Research Institute
2022-2024
Jilin Academy of Agricultural Sciences
2023
Shandong University
2023
Shandong Maternal and Child Health Hospital
2023
Nanjing Agricultural University
2020-2022
Academy of Military Medical Sciences
2019-2021
Summary Grass stomata can balance gas exchange and evaporation effectively in rapidly changing environments via their unique anatomical features. Although the key components of stomatal development Arabidopsis have been largely elucidated over past decade, molecular mechanisms that govern grasses are poorly understood. Via genome editing system T‐ DNA insertion lines, transcriptional factors ( TF s) regulating rice Oryza sativa ) were knocked out. A combination genetic biochemical assays...
Interferons (IFNs) are proteins produced by a variety of cells during the process virus infection. It can activate transcription multiple functional genes in cells, regulate synergistic effect signaling pathways, and mediate biological functions such as antiviral activity immune regulation. The symptoms hosts infected with African swine fever (ASFV) depend on combined interaction between viruses host. However, it is unclear whether IFNs be used an emergency preventive treatment for ASFV....
African swine fever (ASF), caused by the ASF virus (ASFV), is a hemorrhagic and fatal viral disease that affects Eurasian wild boars domestic pigs, posing substantial threat to global pig breeding industry. ASFV, double-stranded DNA virus, possesses large genome containing up 160 open reading frames, most of which exhibit unknown functions. The B125R gene located at 105595-105972 bp site in ASFV-SY18 genome, remains unexplored. In this study, we discovered deletion did not affect recombinant...
Aims: We attempted to identify the genetic epidemiology of hereditary hearing loss among Chinese Han population using next-generation sequencing (NGS). Materials and Methods: The entire length genes GJB2, SLC26A4, GJB3, as well exons 57 additional candidate were sequenced from 116 individuals suffering loss. Results: Thirty potentially causative mutations these 60 identified likely etiologies in 67 cases. In our study, SLC26A4 GJB2 most frequently affected with Collectively, they account for...
We report the genetic analysis of autosomal dominant, nonsyndromic, progressive sensorineural hearing loss in a Chinese family. Using whole exome sequencing, we identified missense variant (c.130C>T, p.R44C) MCM2 gene, which has pro-apoptosis effect and is involved initiation eukaryotic genome replication. This very likely to be disease causing variant. It segregated with this pedigree, was not found dbSNP database or databases genomes SNP population, 76 patients sporadic loss, 145 normal...
The mut-type methylmalonic aciduria (MMA, MIM 251000) is caused by a deficiency of mitochondrial methylmalonyl-CoA mutase (MCM, E.C. 5.4.99.2) activity, which results from defects in the MUT gene. To elucidate mutation spectrum gene Chinese MMA patients, 13 exons gene, including untranslated regions, were analyzed PCR-based sequencing for 42 unrelated patients. All patients found to have at least one mutation. A total 41 mutations identified. Of these mutations, 20 novel ones, nonsense...
Large deletions in mitochondrial DNA (mtDNA) may be involved the pathogenesis of disease. In this study, we investigated relationship between a 4,977-bp deletion genome (ΔmtDNA4977) and severity clinical symptoms patients with disease lacking known point mutations. A total 160 101 healthy controls were recruited for study. The copy numbers ΔmtDNA4977 wild-type mtDNA determined by real-time quantitative PCR analyzed using Spearman's bivariate correlation analysis, t-tests, or one-way ANOVA....
Background: Mitochondrial DNA (mtDNA) content measured by different techniques cannot be compared between studies, and age- tissue-related control values are hardly available. In the present study, we aimed to establish normal reference range of mtDNA copy number in Chinese population. Methods: Two healthy cohorts 200 minors (0.1–18.0 years) adults (18.0–88.0 were recruited. Then, they further categorized into eight age groups. The absolute per cell was a quantitative real-time polymerase...
We report the genetic analysis of a Chinese family with autosomal dominant non-syndromic progressive sensorineural hearing loss. Taking advantage next-generation high-throughput DNA sequencing technology, we combined whole exome capture Sanger direct sequencing. A novel missense mutation in coagulation factor C homolog (COCH) gene was identified consanguineous family. This seventh exon (c.889G>A; p.C162Y) COCH is most probably disease-causing and it segregates disease. The not found single...
Background: Mitochondrial diseases are a group of energy metabolic disorders with multisystem involvements. Variable clinical features present major challenge in pediatric diagnoses. We summarized the spectrum m.3243A>G mutation Chinese patients, to define common manifestations and study correlation between heteroplasmic degree severity disease. Methods: Clinical data one-hundred patients symptomatic mitochondrial disease harboring from 2007 2013 were retrospectively reviewed. Detection...
African swine fever virus (ASFV) causes acute hemorrhagic in domestic pigs and wild boars, resulting incalculable economic losses to the pig industry. As mechanism of viral infection is not clear, protective antigens have been discovered or identified. In this study, we determined that p30, pp62, p72, CD2v proteins were all involved T cell immune response live infected with ASFV, among which p72 pp62 strongest. Panoramic scanning was performed on epitopes protein, three high-frequency...
One tumor-targeting, phenylboronic acid-functionalized polyaminoglycoside (SS-HPT-P) was proposed as a safe and effective CRISPR/Cas9 delivery system for the treatment of carcinoma.
African swine fever is an acute, haemorrhagic and contagious disease of pigs caused by virus (ASFV), which has a great impact on the pig farming industry related international trade. Understanding response processes various tissues in after ASFV infection may help to address current major concerns, such as exploration key genes for vaccine development, cooperative mechanism host possibility establishing active herd immunity. able infect core associated with acute death. RNA protein samples...
African swine fever (ASF) is an acute infectious disease of domestic pigs and wild boars caused by the virus (ASFV), with up to a 100% case fatality rate. The development vaccine for ASFV hampered fact that function many genes in genome still needs be discovered. In this study, previously unreported E111R gene was analyzed identified as early-expressed highly conserved across different genotypes ASFV. To further explore gene, recombinant strain, SY18ΔE111R, constructed deleting lethal SY18...
The prevalence of CGG repeat expansion mutation in FMR1 gene varies among different populations. In this study, we investigated the women reproductive age from northern China. A total 11,891 pre-conceptional or pregnant women, including 5037 and 7357 with history spontaneous abortion induced due to delayed growth embryos, were recruited. number repeats was measured by TRP-PCR method. We also offered genetic counseling prenatal diagnosis carrying pre-mutation full alleles. China 1/410, higher...
Vibrio parahaemolyticus is a marine pathogen thought to be the leading cause of seafood-borne gastroenteritis globally, urgently requiring efficient management methods. V. encodes 12 resistance/nodulation/division (RND) efflux systems. However, research on these systems still in its infancy. In this study, we discovered that inactivation VmeL, membrane fusion protein within RND systems, led reduction ability biofilm formation. Further results displayed decreased capacity Congo red binding...