A5044650493- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- SARS-CoV-2 detection and testing
- Nanocluster Synthesis and Applications
- Kawasaki Disease and Coronary Complications
- Listeria monocytogenes in Food Safety
- Inflammasome and immune disorders
- Additive Manufacturing and 3D Printing Technologies
- Modular Robots and Swarm Intelligence
- Advanced Chemical Sensor Technologies
- Advanced Nanomaterials in Catalysis
- Aquaculture disease management and microbiota
- Perovskite Materials and Applications
- Product Development and Customization
- Advanced Photocatalysis Techniques
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Quantum Dots Synthesis And Properties
- Animal Nutrition and Physiology
- Glycosylation and Glycoproteins Research
Northwest A&F University
2023-2025
Institute of Food Science & Technology
2025
Hangzhou Medical College
2024
Zhejiang Provincial People's Hospital
2024
How timely identification and determination of pathogen species in pathogen-contaminated foods are responsible for rapid accurate treatments food safety accidents. Herein, we synthesize four aggregation-induced emissive nanosilicons with different surface potentials hydrophobicities by encapsulating tetraphenylethylene derivatives differing functional groups. The prepared utilized as receptors to develop a nanosensor array according their distinctive interactions pathogens the simultaneous...
Bimodal-type multiplexed immunoassays with complementary mode-based correlation analysis are gaining increasing attention for enhancing the practicability of lateral flow immunoassay (LFIA). Nonetheless, restriction in visually indistinguishable multitargets induced by a single fluorescent color and difficulty acceptor ineffectual fluorescence quenching due to various spectra multiple different donors impede further execution colorimetric–fluorescence bimodal-type LFIAs. Herein, precise...
Immunochromatography (ICA) remains untapped toward enhanced sensitivity and applicability for fulfilling the nuts bolts of on-site food safety surveillance. Herein, we report a fortified dual-spectral overlap with colorimetric/fluorescence dual-response ICA bimodal-type gentamicin (Gen) monitoring by employing polydopamine (PDA)-coated AuNPs (APDA) simultaneously serving as colorimetric reporter fluorescence quencher. Availing response that originated from PDA layer, resultant APDA exhibits...
With the global limited food resources receiving grievous damage from frequent climate changes and ascending demand resulting increasing population growth, perovskite nanocrystals with distinctive photoelectric properties have emerged as attractive prospective luminogens for exploitation of rapid, easy operation, low cost, highly accurate, excellently sensitive, good selective biosensors to detect foodborne hazards in practices. Perovskite demonstrated supreme advantages luminescent...
Nanobodies (Nbs) serve as powerful tools in immunoassays. However, their small size and monovalent properties pose challenges for practical application. Multimerization emerges a significant strategy to address these limitations, enhancing the utilization of nanobodies Herein, we report construction Salmonella-specific fenobody (Fb) through fusion nanobody ferritin, resulting self-assembled 24-valent nanocage-like structure. The exhibits 35-fold increase avidity compared conventional while...
Aflatoxin B1 (AFB1) is considered a critical analyte in food safety testing due to its widespread contamination and high toxicity. In this study, ratiometric fluorescent immunosensor with synergistic catalysis by natural enzymes nanozymes (N-N-RF-immunosensor) was constructed for the rapid detection of AFB1. Copper oxide zeolitic imidazolate framework composite nanomaterials (CuO/ZIF-8 NCM) peroxidase oxidase activities were prepared. A fluorescence signal generated through cocatalytic...
The exploitation of stable, high-affinity, and low-cost nanoprobes is essential to develop immunoassays for real-time monitoring foodborne pathogens, so as safeguard human health. possible interaction the Fc fragment antibodies with spA protein on Staphylococcus aureus will result in unexpected interference. To address this consideration, we described herein first time development nanobodies that by definition are devoid fraction. These directed against Cronobacter sakazakii (C. sakazakii)...
Nanobodies have gained widespread application in immunoassays. However, their small size presents a significant challenge achieving effective immobilization and optimal sensitivity. Here, we present novel "one-for-two"-oriented platform based on an organism-bispecific nanobody (O-BsNb) scaffold, enabling highly sensitive detection of two bacterial pathogens. Through genetic engineering, bispecific (BsNb) was engineered, targeting Salmonella spp. Vibrio parahaemolyticus. The O-BsNb scaffold...
Nanobodies (Nbs) are widely used in immunoassays with the advantages of small size and high stability. Here, nanobody employed as surrogate aflatoxin antigen recognition mechanism antiaflatoxin mAb was studied by molecular modeling, which verified feasibility Nbs substitutes. On this basis, a nanobody-alkaline phosphatase fusion protein (Nb-AP) constructed, highly sensitive "on-off-on" fluorescent immunosensor (OFO-FL immunosensor) based on calcein/Ce3+ system developed for quantification....
Protein-nanoparticle interactions play a crucial role in both biomedical applications and the biosafety assessment of nanomaterials. Here, we found that nanobodies can induce citrate-capped gold nanoparticles (AuNPs) to aggregate into large clusters. Subsequently, explored mechanism behind this aggregation proposed "gold nucleation mechanism" explain phenomenon. Building on observation, developed one-step label-free colorimetric method based nanobody-induced AuNP aggregation. When bind...
Kawasaki disease (KD) is a kind of autoimmune with systemic vasculitis as the main pathological change. It critical to explore potential new therapeutic agents address KD and its complications. Ursolic acid (UA) pentacyclic triterpene (PT) carboxylic that has number important pharmacological activities, however, possible effects UA on progression mechanism are still unclear. Here we investigated KD. We revealed improved arterial injury in mice. vascular inflammation In addition, suppressed...