A5078357918- Malaria Research and Control
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Travel-related health issues
- Computational Drug Discovery Methods
- Drug-Induced Hepatotoxicity and Protection
- Parasites and Host Interactions
- COVID-19 epidemiological studies
- Drug Transport and Resistance Mechanisms
- Insect Pest Control Strategies
- Vector-borne infectious diseases
- HIV/AIDS drug development and treatment
- Viral Infections and Outbreaks Research
- Research on Leishmaniasis Studies
- Antibiotic Resistance in Bacteria
- Hemoglobinopathies and Related Disorders
- Trypanosoma species research and implications
- Insect Resistance and Genetics
- Neonatal Health and Biochemistry
- Traditional and Medicinal Uses of Annonaceae
- Zoonotic diseases and public health
- Insect symbiosis and bacterial influences
- Dengue and Mosquito Control Research
- Vibrio bacteria research studies
- Blood disorders and treatments
Institut de Médecine Tropicale du Service de Santé des Armées
2016-2025
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2013-2025
Méditerranée Infection Foundation
2018-2025
Aix-Marseille Université
2016-2025
Assistance Publique Hôpitaux de Marseille
2021-2025
Institut de Recherche pour le Développement
2015-2024
Biomedical Research Institute
2023
University of Djibouti
2023
Service de Santé des Armées
2003-2023
Hôpital d'Instruction des Armées Bégin
2023
Abstract Background An accurate diagnosis is essential for the rapid and appropriate treatment of malaria. The accuracy histidine-rich protein 2 (PfHRP2)-based diagnostic test (RDT) Palutop+4® was assessed here. One possible factor contributing to failure detect malaria by this diversity parasite PfHRP2 antigens. Methods detection with RDT carried out. pfhrp2 pfhrp3 genes were amplified sequenced from 136 isolates Plasmodium falciparum that collected in Dakar, Senegal 2009 2011. DNA...
Abstract Anopheles stephensi mosquitoes share urban breeding sites with Aedes aegypti and Culex quinquefasciatus in the Republic of Djibouti. We present evidence that A. might be responsible for an increase malaria incidence this country. also document resistance Plasmodium falciparum to dihydroartemisinin/piperaquine.
Duffy blood group polymorphisms are important in areas where Plasmodium vivax is present because this surface antigen thought to act as a key receptor for parasite. In the study, genotyping was performed febrile uninfected and P. vivax-infected patients living city of Nouakchott, Mauritania. identified by real-time PCR. The genotypes were determined standard PCR followed sequencing promoter region exon 2 gene 277 individuals. Fisher's exact test order assess significance variables. Moorish...
Background Dengue fever epidemic dynamics are driven by complex interactions between hosts, vectors and viruses. Associations climate dengue have been studied around the world, but results shown that impact of can vary widely from one study site to another. In French Guiana, climate-based models not available assist in developing an early warning system. This aims evaluate potential using oceanic atmospheric conditions help predict outbreaks Guiana. Methodology/Principal Findings Lagged...
Aedes aegypti mosquitoes are established throughout Nouakchott, Mauritania, where its insecticide resistance status is unknown and dengue has become endemo-epidemic since 2014. Eggs were collected using ovitraps at 12 sites in five districts of August 2024. Adult females larvae the F1 generation used for bioassays. Permethrin, deltamethrin, bendiocarb, malathion evaluated discriminating concentrations. Larval assays carried out seven concentrations with Bacillus thuriengensis var israelensis...
Abstract Background The emergence of Plasmodium falciparum resistance to most anti-malarial compounds has highlighted the urgency develop new drugs and clarify mechanisms currently used. Among them, doxycycline is used alone for malaria chemoprophylaxis or in combination with quinine artemisinin derivatives treatment. molecular action P. have not yet been clearly defined, particularly at protein level. Methods A proteomic approach was analyse expression changes schizont stage malarial...
ABSTRACT Polymorphisms in the Plasmodium falciparum crt (Pf ), Pf mdr1 , and mrp genes were not significantly associated with quinine (QN) 50% inhibitory concentrations (IC 50 s) 23 strains of . An increased number DNNND repeats nhe-1 microsatellite ms4760 was an IC QN ( P = 0.0007). Strains only one repeat more susceptible to (mean 154 nM). two had intermediate susceptibility 548 three reduced 764 Increased numbers NHNDNHNNDDD a decreased 0.0020). profile 7 for (ms4760-7) The determination...
In December 2010, a Plasmodium vivax malaria outbreak occurred among French forces involved in mission to control illegal gold mining Guiana. The findings of epidemiological and entomological investigations conducted after this are presented here. Data related cases reported the armed surveillance system were collected during epidemic period from 2010 April 2011. A retrospective cohort study was identify presumed contamination sites. Anopheles mosquitoes sampled at identified sites using...
On 16 September 2016, the World Health Organization confirmed a Rift Valley fever (RVF) outbreak in Niger. Epidemiological surveillance was reinforced among French Armed Forces deployed Niger and bordering countries: Chad, Mali Burkina Faso. 26 October, probable case of RVF reported service member sampled 3 weeks earlier. At time result reported, patient on vacation Martinique. An epidemiological investigation conducted to confirm this identify other cases. Finally, not confirmed, but three...
Abstract Background As a result of the widespread resistance to chloroquine and sulphadoxine-pyrimethamine, artemisinin-based combination therapy (ACT) (including artemether-lumefantrine artesunate-amodiaquine) has been recommended as first-line anti-malarial regimen in Senegal since 2006. Intermittent preventive treatments with drugs based on sulphadoxine-pyrimethamine are also given children or pregnant women once per month during transmission season. Since 2006, there have very few...
The in vitro activity of ferroquine (FQ) (SR97193), a 4-aminoquinoline antimalarial compound that contains ferrocenic nucleus, against 15 Plasmodium falciparum strains was assessed and compared with those chloroquine (CQ), quinine (QN), monodesethylamodiaquine (MDAQ), mefloquine (MQ). These were genotyped for polymorphisms quinoline resistance-associated genes such as Pfcrt, Pfmdr1, Pfmrp, Pfnhe-1. FQ highly active CQ-resistant parasites or reduced susceptibility to QN, MDAQ, MQ....
The geometric mean 50% inhibitory concentration (IC50) for Proveblue, a methylene blue complying with the European Pharmacopoeia, was more active on 23 P. falciparum strains than chloroquine, quinine, mefloquine, monodesethylamodiaquine, and lumefantrine. We did not find significant associations between Proveblue IC50 polymorphisms in pfcrt, pfmdr1, pfmdr2, pfmrp, pfnhe-1 genes or copy numbers of pfmdr1 pfmdr2 genes, all which are involved antimalarial resistance.
We have analyzed the profiles of 23 Plasmodium falciparum strains for their in vitro chemosusceptibilities to piperaquine (PPQ), dihydroartemisinin (DHA), chloroquine, monodesethylamodiaquine, quinine, mefloquine, lumefantrine, atovaquone, pyrimethamine, and doxycycline (DOX) association with polymorphisms genes involved quinoline resistance (Plasmodium crt [pfcrt], pfmdr1, pfmrp, pfnhe). The 50% inhibitory concentrations (IC(50)s) PPQ ranged from 29 98 nM (geometric mean = 57.8 nM, 95%...
Abstract Background Case management of imported malaria within the context pre-elimination is increasingly considered to be relevant because risk resurgence. The assessment importation would provide key data i) select countries with propitious conditions for phase and ii) predict its feasibility. Recently, a sero-prevalence study in Djibouti indicated low prevalence, which implementation pre-elimination, but on extent remain unknown. Methods Djiboutian plasmodial populations were analysed...
In French Guiana, malaria vector control and prevention relies on indoor residual spraying distribution of long lasting insecticidal nets. These measures are based solid epidemiological evidence but reveal a poor understanding the vector. The current study investigated behaviour both vectors humans in relation to ongoing strategies. 2012 2013, Anopheles mosquitoes were sampled outdoors at different seasons various time slots. collected identified screened for Plasmodium infection. Data human...
Doxycycline is used in combination with quinine for malaria treatment or alone chemoprophylaxis. However, the occurrence of after doxycycline chemoprophylaxis has been reported. Identification genetic determinants that contribute to susceptibility Plasmodium falciparum will be important detection and surveillance resistance.Sequence analysis 11 genes (pftufA, pfEF-TS, pfmdt, pftetQ, pfrps3, pfrps7, pfrps8, pfrps9, pfrps11, pfrps14, pfrps17) evaluation pfmdt pftetQ copy numbers by...
Journal Article Malaria prevalence and morbidity among children reporting at health facilities in Nouakchott, Mauritania Get access Khadijetou Mint Lekweiry, Lekweiry aLaboratoire de Biotechnologies, Faculté des Sciences et Techniques, Université BP 5026, MauritaniabLaboratoire Aliments, Environnement Santé (LAES), Cadi Ayyad, Marrakech, Morocco Search for other works by this author on: Oxford Academic PubMed Google Scholar Leonardo K. Basco, Basco cUnité Recherche en Biologie Epidémiologie...
Abstract Background In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy (ACT) as first-line treatment for uncomplicated malaria. Since introduction of ACT, there have been very few reports on level resistance P. falciparum to anti-malarial drugs. To determine whether parasite susceptibility has affected by new policies, an ex vivo and drug molecular marker study was conducted local isolates obtained from Centre de santé Elizabeth Diouf...
In 2002, the World Health Organization recommended that artemisinin-based combination therapy (ACT) be used to treat uncomplicated malaria. Dihydroartemisinin-piperaquine and artesunate-pyronaridine are two of these new combinations. The aim present work was assess distribution in vitro values pyronaridine (PND) piperaquine (PPQ) define a cut-off for reduced susceptibility anti-malarial drugs. range 50% inhibitory concentration (IC50) PND PPQ were determined 313 isolates obtained between...